LOCUS       sequence-335974-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This gene was cloned into the gateway middle entry vector. It can be
            used for generating antisense riboprobe or gene expression using a 
            gateway system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Silic MR, Murata SH, Park SJ, Zhang G
  TITLE     Evolution of inwardly rectifying potassium channels and their gene 
            expression in zebrafish embryos.
  JOURNAL   Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.425.
  PUBMED    34558132
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2021 Sep 23. doi: 10.1002/dvdy.425."
COMMENT     Sequence Label: pDONR221-kcnj5
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    4..103
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tggcaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgataag
       61 caatgctttc ttataatgcc aactttgtac aagaaagctg ggtctcaggg attctcctcc
      121 aggcggtttg ccgagccgtt gttggtctcc ggcggggtgt cggcgtcgtg cttggcctgg
      181 tcctcggtgg tctggaacgg gtccgttgtg tgcgccctga ggtccgggct gagcaggggc
      241 aggtgcggga gcagggggct ctcttttaac cgggcggaga gttctttagc gctacaggtc
      301 ggggtgttgg tttcgtatat gtcgtgaaag ttgttgtagt cgacttcgta gaagcccttt
      361 tccagcgaaa gcaccggcgt gaagcggaag ccccacagca cctccgagtc caagtacgag
      421 ctgcgcgcct gacacgtcat gcctgtagct tcaaccatgc cttctaagat gaccacaatc
      481 tcgaactctt ccttctccat ctgtgcccgc gacatctccc agaaaggact cttctcgttg
      541 atctcatgtg agatgataag aggagatacc aagaataagc gatcgtctcc tgtgtcgaag
      601 ccaatgttga tgtctgtctg attgagcgga ataaactcgc cctccttggt ctgctgcgag
      661 cggatgagtt tggcccgaat tgacgcttcc acaatgtggg aattcctcaa gtcgcccacc
      721 ctgaacatc
//
