LOCUS       sequence-336068-        1442 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector to express sgRNA resistant HA tagged human GPAT4 
            with mutation in CHP1 binding site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Zhu XG, Nicholson Puthenveedu S, Shen Y, La K, Ozlu C, Wang T, 
            Klompstra D, Gultekin Y, Chi J, Fidelin J, Peng T, Molina H, Hang 
            HC, Min W, Birsoy K
  TITLE     CHP1 Regulates Compartmentalized Glycerolipid Synthesis by 
            Activating GPAT4.
  JOURNAL   Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi: 
            10.1016/j.molcel.2019.01.037. Epub 2019 Mar 4.
  PUBMED    30846317
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.01.037"; journalName: "Mol Cell"; date: 
            "2019-04-4- 4"; volume: "74"; issue: "1"; pages: "45-58.e7"
COMMENT     Sequence Label: pMXS-IRES-Blast HA-GPAT4-Delta-Ns5
FEATURES             Location/Qualifiers
     source          1..1442
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1395..1421
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gccggatcta gctagttaat taagccacca tgttcctgtt gctgcctttt gatagcctga
       61 ttgtcaacct tctgggcatc tccctgactg tcctcttcac cctccttctc gttttcatca
      121 tagtgccagc catttttgga gtctcctttg gtatccgcaa actctacatg aaaagtctgt
      181 taaaaatctt tgcgtgggct accttgagaa tggagcgagg agccaaggag aagaaccacc
      241 agctttacaa gccctacacc aacggaatca ttgcaaagga tcccacttca ctagaagaag
      301 agatcaaaga gattcgtcga agtggtagta gtaaggctct ggacaacact ccagagttcg
      361 agctccggaa aggaatggag accattatgg atgatgaggt gacaaagaga ttctcagcag
      421 aagaactgga gtcctggaac ctgctgagca gaaccaatta taacttccag tacatcagcc
      481 ttcggctcac ggtcctgtgg gggttaggag tgctgattcg gtactgcttt ctgctgccgc
      541 tcaggatagc actggctttc acagggatta gccttcttgt tgttggaacc acggttgttg
      601 gatacttgcc aaatgggagg tttaaggagt tcatgagtaa acatgttcac ttaatgtgtt
      661 accggatctg cgtgcgagcg ctgacagcca tcatcaccta ccatgacagg gaaaacagac
      721 caagaaatgg tggcatctgt gtggccaatc atacctcacc gatcgatgtg atcatcttgg
      781 ccagcgatgg ctattatgcc atggtgggtc aagtgcacgg gggactcatg ggtgtgattc
      841 agagagcaat ggttaaagca tgtccccatg tctggtttga gcgctcggaa gtgaaggatc
      901 gccacctggt ggctaagaga ctgactgaac atgtgcaaga taaaagcaag ctgcctatcc
      961 tcatcttccc agaaggaacc tgcatcaata atacatcggt gatgatgttc aaaaagggaa
     1021 gttttgaaat tggagccaca gtttaccctg ttgctatcaa gtatgaccct caatttggcg
     1081 atgccttctg gaacagcagc aaatacggga tggtgacgta cctgctgcga atgatgacca
     1141 gctgggccat tgtctgcagc gtgtggtacc tgcctcccat gactagagag gcagatgaag
     1201 atgctgtcca gtttgcgaat agggtgaaat ctgccattgc caggcaggga ggacttgtgg
     1261 acctgctgtg ggatgggggc ctgaagaggg agaaggtgaa ggacacgttc aaggaggagc
     1321 agcagaagct gtacagcaag atgatcgtgg ggaaccacaa ggacaggagc cgctccggag
     1381 gttcaggtgg atcataccca tacgatgttc cagattacgc ttgagctacg taaattccgc
     1441 cc
//
