LOCUS       sequence-33607-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, 
            Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, 
            Unger-Wallace E, Sander JD, Muller-Lerch F, Fu F, Pearlberg J, 
            Goebel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate
            AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK
  TITLE     Rapid ""open-source"" engineering of customized zinc-finger 
            nucleases for highly efficient gene modification.
  JOURNAL   Mol Cell. 2008 Jul 25. 31(2):294-301.
  PUBMED    18657511
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Jul 25. 31(2):294-301."
COMMENT     Sequence Label: pitrm1_R (OZ516)
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..70
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     rep_origin      472..830
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(559..578)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     769..790
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 acattgtgca aaggccctga gaaaaatcca agctttggcg gccgctgact acaaagacga
       61 tgatgacaaa tctagacccg gggagcgccc cttccagtgt cgcatttgca tgcggaactt
      121 ttcgaagcac agcaacctta cccgccatac ccgtactcat accggtgaaa aaccgtttca
      181 gtgtcggatc tgtatgcgaa atttctccca ggggagcaac ttgatcaggc atctacgtac
      241 gcacaccggc gagaagccat tccaatgccg aatatgcatg cgcaacttca gtcagcggaa
      301 caacctgggc cggcacctaa aaacccacct gaggggatcc tctgcacagt agtaactaga
      361 gactagaaaa aggccgacaa gtcccgctcc gctgaagatc ctggcgtaat agcgaagagg
      421 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
      481 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
      541 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
      601 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
      661 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
      721 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
      781 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta t
//
