LOCUS       sequence-336084-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 Y113N mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_Y113N_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..46)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(200..788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(280..299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(959..1750,1751..1819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1582..1601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1820..1924)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1999..2018)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2190..2569
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2571..2769
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2724..2744
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2787..2967
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3014..3139
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3632..3865
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4050..4094
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4392..4509
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4645..5823
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4876..5814
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5771..5791
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     5840..5859
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5840..5858
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5922..6944
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5922..6944
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6954..7010
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(8564..8597)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8622..8802
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8828..8845)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8834..9058
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9104..9532
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9191..9210)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9401..9422
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9541..9561)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9546..9875
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9726..9861
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9923..9970
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9989..10363
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10493..10614
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10530..10549)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10584..10603
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10663..10679)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10663..10679)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10676..10698)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10687..10703
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10711..10717,10718..10735,10736..10741))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10756..10777
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
       61 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
      901 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
      961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     1801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     1861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     1921 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     1981 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     2041 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     2101 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     2161 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
     2221 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     2281 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     2341 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     2401 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     2461 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     2521 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     2581 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     2641 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     2701 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     2761 tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
     2821 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     2881 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     2941 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     3001 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     3061 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     3121 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     3181 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     3241 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     3301 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     3361 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     3421 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     3481 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     3541 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     3601 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     3661 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     3721 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     3781 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     3841 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     3901 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     3961 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     4021 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     4081 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     4141 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     4201 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     4261 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     4321 ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta
     4381 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     4441 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     4501 caaaattttc gggtttatta cagggacagc agagatccag tttggttaat taatcgataa
     4561 gctttgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta atggaccttc
     4621 taggtcttga aaggagtggg aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg
     4681 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
     4741 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     4801 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     4861 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
     4921 tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc
     4981 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg
     5041 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
     5101 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
     5161 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac
     5221 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
     5281 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
     5341 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
     5401 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
     5461 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
     5521 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
     5581 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt
     5641 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
     5701 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     5761 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg
     5821 tgaggaatta gcttggtact aatacgactc actataggga gacccaagct ggctaggtaa
     5881 gcttgatatc gaattcctgc agcccggggg atcccgccac catgaaaaag cctgaactca
     5941 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     6001 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     6061 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     6121 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     6181 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     6241 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     6301 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     6361 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     6421 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     6481 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     6541 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     6601 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     6661 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     6721 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     6781 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     6841 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     6901 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaaggatcc ggcgcaacaa
     6961 acttctctct gctgaaacaa gccggagatg tcgaagagaa tcctggaccg atgcgtatcc
     7021 ccgtagatgc cagcacgagc cgccgcttca cgccgccttc caccgcgctg agcccaggca
     7081 agatgagcga ggcgttgccg ctgggcgccc cggacgccgg cgctgccctg gccggcaagc
     7141 tgaggagcgg cgaccgcagc atggtggagg tgctggccga ccacccgggc gagctggtgc
     7201 gcaccgacag ccccaacttc ctctgctccg tgctgcctac gcactggcgc tgcaacaaga
     7261 ccctgcccat cgctttcaag gtggtggccc taggggatgt tccagatggc actctggtca
     7321 ctgtgatggc tggcaatgat gaaaacaact cggctgagct gagaaatgct accgcagcca
     7381 tgaagaacca ggttgcaaga tttaatgacc tcaggtttgt cggtcgaagt ggaagaggga
     7441 aaagcttcac tctgaccatc actgtcttca caaacccacc gcaagtcgcc acctaccaca
     7501 gagccatcaa aatcacagtg gatgggcccc gagaacctcg aagacatcgg cagaaactag
     7561 atgatcagac caagcccggg agcttgtcct tttccgagcg gctcagtgaa ctggagcagc
     7621 tgcggcgcac agccatgagg gtcagcccac accacccagc ccccacgccc aaccctcgtg
     7681 cctccctgaa ccactccact gcctttaacc ctcagcctca gagtcagatg caggatacaa
     7741 ggcagatcca accatcccca ccgtggtcct acgatcagtc ctaccaatac ctgggatcca
     7801 ttgcctctcc ttctgtgcac ccagcaacgc ccatttcacc tggacgtgcc agcggcatga
     7861 caaccctctc tgcagaactt tccagtcgac tctcaacggc acccgacctg acagcgttca
     7921 gcgacccgcg ccagttcccc gcgctgccct ccatctccga cccccgcatg cactatccag
     7981 gcgccttcac ctactccccg acgccggtca cctcgggcat cggcatcggc atgtcggcca
     8041 tgggctcggc cacgcgctac cacacctacc tgccgccgcc ctaccccggc tcgtcgcaag
     8101 cgcagggagg cccgttccaa gccagctcgc cctcctacca cctgtactac ggcgcctcgg
     8161 ccggctccta ccagttctcc atggtgggcg gcgagcgctc gccgccgcgc atcctgccgc
     8221 cctgcaccaa cgcctccacc ggctccgcgc tgctcaaccc cagcctcccg aaccagagcg
     8281 acgtggtgga ggccgagggc agccacagca actcccccac caacatggcg ccctccgcgc
     8341 gcctggagga ggccgtgtgg aggccctact gactagctaa gttagaacta tttcctggct
     8401 gttacgcgtg attgacctgg ctactgacgg gcttgttaaa cagtgaaccg cccgccccac
     8461 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcgaacaa aagaaaaggg
     8521 gggactggaa gggctaattc actcccaacg aagacaagat atcataactt cgtatagcat
     8581 acattatacg aagttatcgc ctagctggtc cggactgtac tgggtctctc tggttagacc
     8641 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8701 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8761 gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc
     8821 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     8881 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     8941 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     9001 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     9061 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     9121 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     9181 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     9241 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     9301 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     9361 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     9421 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     9481 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     9541 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     9601 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     9661 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     9721 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     9781 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     9841 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     9901 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
     9961 ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct
    10021 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
    10081 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
    10141 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
    10201 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
    10261 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
    10321 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
    10381 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
    10441 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
    10501 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    10561 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10621 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
    10681 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    10741 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    10801 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    10861 aggcg
//
