LOCUS       sequence-336390-       10951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of syt1 in cultured neurons by lentiviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10951)
  AUTHORS   Bradberry MM, Courtney NA, Dominguez MJ, Lofquist SM, Knox AT, 
            Sutton RB, Chapman ER
  TITLE     Molecular Basis for Synaptotagmin-1-Associated Neurodevelopmental 
            Disorder.
  JOURNAL   Neuron. 2020 Jul 8;107(1):52-64.e7. doi: 
            10.1016/j.neuron.2020.04.003. Epub 2020 May 1.
  PUBMED    32362337
REFERENCE   2  (bases 1 to 10951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2020.04.003"; journalName: "Neuron"; date: 
            "2020-07-8- 8"; volume: "107"; issue: "1"; pages: "52-64.e7"
COMMENT     Sequence Label: pFSynW Syt1 D365E IRES mRuby3
FEATURES             Location/Qualifiers
     source          1..10951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3048
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3065..3074
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4351..4922
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4372..4924
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4516..4533)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4743..4762
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    5643..6231
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5696..5716)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6114..6125)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6234..6250)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6235..6251)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6756..6936
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6962..6979)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6968..7192
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7238..7666
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7325..7344)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7535..7556
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7675..7695)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7680..8009
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7860..7995
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7922..7941
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8057..8104
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8123..8497
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8627..8748
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8664..8683)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8718..8737
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8797..8813)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8797..8813)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8810..8832)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8821..8837
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8845..8851,8852..8869,8870..8875))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8890..8911
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9028..9045)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9199..9787)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9279..9298)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9958..10749,10750..10818))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10581..10600
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10819..10923)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aagtgcaagt ggttttagga ccaggatgag gcggggtggg
     2641 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2701 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2761 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2821 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgtaaac tccccttccc
     2881 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2941 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     3001 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcaggt acggctagcg
     3061 gatcgccacc atggtgagtg ccagtcatcc tgaggccctg gccgcccctg tcaccactgt
     3121 tgcgaccctt gtcccacaca atgccactga gccagccagt cctggggaag ggaaggaaga
     3181 tgccttttcc aagctgaagc agaagtttat gaatgagctg cataaaattc cattgccacc
     3241 gtgggcctta atagccatag ccatagttgc ggtcctttta gtcgtaacct gctgcttttg
     3301 tgtctgtaag aaatgtttgt tcaaaaagaa aaacaagaag aaggggaagg aaaagggagg
     3361 aaagaacgcc attaacatga aagacgtgaa agacttaggg aagaccatga aggatcaggc
     3421 ccttaaggat gacgatgctg aaaccggact gactgatgga gaagaaaagg aagagcccaa
     3481 ggaagaggag aaactgggaa agctccaata ttcactggac tatgacttcc agaataacca
     3541 gctgttggtg ggaatcatcc aggctgctga actgcccgcc ctggacatgg ggggtacatc
     3601 cgatccatac gtcaaagtct tcctgctgcc tgacaaaaag aagaaatttg agactaaagt
     3661 ccaccggaaa accctcaatc cagtcttcaa tgaacaattt actttcaagg taccctactc
     3721 ggaattaggt ggcaaaaccc tggtgatggc tgtgtatgac tttgatcgct tctccaagca
     3781 cgacatcatc ggagagttca aagttcctat gaacaccgtg gattttggcc atgtgaccga
     3841 ggagtggcgc gatctccaga gcgctgagaa agaagagcaa gagaaactgg gtgacatctg
     3901 cttctccctc cgctacgtcc ctactgccgg caaactgact gttgtcattc tggaagccaa
     3961 gaacctgaag aagatggatg tgggtggctt atctgatccc tacgtgaaga ttcacctgat
     4021 gcagaacggt aagaggctga agaagaaaaa gacgacgatt aagaagaaca cactcaaccc
     4081 ctactacaac gagtccttca gctttgaagt tccgttcgag caaatccaga aagtgcaagt
     4141 ggtggtaact gttttggact atgagaagat tggcaagaac gacgccatcg gcaaagtctt
     4201 cgttggttac aacagcactg gggcggagct gcgacactgg tcagacatgc tggccaaccc
     4261 ccggcgaccc atcgcacagt ggcacactct gcaggtagag gaggaggtcg atgccatgct
     4321 ggctgtcaag aagtaaggcg gccgcaattc cccctctccc tcccccccct aacgttactg
     4381 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     4441 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     4501 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     4561 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     4621 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     4681 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     4741 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     4801 gtatgggatc tgatctgggg cctcggtaca catgctttac atgtgtttag tcgaggttaa
     4861 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     4921 aatatggtgt ctaagggcga agagctgatc aaggaaaata tgcgtatgaa ggtggtcatg
     4981 gaaggttcgg tcaacggcca ccaattcaaa tgcacaggtg aaggagaagg cagaccgtac
     5041 gagggagtgc aaaccatgag gatcaaagtc atcgagggag gacccctgcc atttgccttt
     5101 gacattcttg ccacgtcgtt catgtatggc agccgtacct ttatcaagta cccggccgac
     5161 atccctgatt tctttaaaca gtcctttcct gagggtttta cttgggaaag agttacgaga
     5221 tacgaagatg gtggagtcgt caccgtcacg caggacacca gccttgagga tggcgagctc
     5281 gtctacaacg tcaaggtcag aggggtaaac tttccctcca atggtcccgt gatgcagaag
     5341 aagaccaagg gttgggagcc taatacagag atgatgtatc cagcagatgg tggtctgaga
     5401 ggatacactg acatcgcact gaaagttgat ggtggtggcc atctgcactg caacttcgtg
     5461 acaacttaca ggtcaaaaaa gaccgtcggg aacatcaaga tgcccggtgt ccatgccgtt
     5521 gatcaccgcc tggaaaggat cgaggagagt gacaatgaaa cctacgtagt gcaaagagaa
     5581 gtggcagttg ccaaatacag caaccttggt ggtggctaaa attcgatatc aagcttatcg
     5641 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     5701 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     5761 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     5821 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     5881 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     5941 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6001 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     6061 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     6121 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     6181 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     6241 gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     6301 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     6361 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6421 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     6481 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     6541 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     6601 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     6661 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     6721 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     6781 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6841 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6901 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     6961 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     7021 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     7081 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     7141 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     7201 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     7261 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     7321 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7381 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7441 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7501 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7561 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7621 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     7681 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7741 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7801 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     7861 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7921 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     7981 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     8041 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     8101 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     8161 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     8221 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     8281 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     8341 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     8401 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     8461 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     8521 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     8581 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     8641 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8701 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     8761 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     8821 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8881 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8941 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9001 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9061 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9121 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9181 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9241 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9301 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9361 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9421 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9481 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9541 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9601 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9661 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9721 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9781 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9841 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9901 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9961 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10021 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10081 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10141 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10201 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10261 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10321 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10381 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10441 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10501 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10561 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10621 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10681 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10741 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10801 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10861 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10921 gcgcacattt ccccgaaaag tgccacctga c
//
