LOCUS       sequence-336455-        9012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Alpha2 Basic cloning vector GB2.0 compatible P[acman]-derived 
            backbone. Allows for copy induction from low to high using AutoFOS 
            or similar. Requires EPI300 bacterial strain (DH10B Derived) or 
            similar. uses blue-white screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 9012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: P[acman]-Alpha2
FEATURES             Location/Qualifiers
     source          1..9012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(411..1382)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(1382..2557)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    2883..3133
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(3136..3891)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      3982..4201
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      4288..4923
                     /label=oriV
                     /note="incP origin of replication"
     primer_bind     complement(6149..6168)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6160..6975
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             6160..6975
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6233..6252)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     protein_bind    7106..7175
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    join(7390..7420,7421..7622)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        7658..7705
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7759..7777
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     7759..7776
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    7784..7840
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7842..7861)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7843..7861)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7868..7885)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7868..7884)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7877..7899)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8093..8112)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(8145..8699,8700..8730)
                     /label=P element 5' end
ORIGIN
        1 caagtgggac cgtggtccca gaccgataat cagaccgaca acacgagtgg gatcgtggtc
       61 ccagactaat aatcagaccg acgatacgag tgggaccgtg gtcccagact aataatcaga
      121 ccgacgatac gagtgggacc gtggtcccag tctgattatc agaccgacga tacgagtggg
      181 accgtggtcc cagactaata atcagaccga cgatacgagt gggaccgtgg tcccagacta
      241 ataatcagac cgacgatacg agtgggaccg tggtcccagt ctgattatca gaccgacgat
      301 acaagtggaa cagtgggccc agagagaata ttcaggccag ttatgctttc tggcctgtaa
      361 caaaggacat taagtaaaga cagataaacg tagactaaaa cgtggtcgca tcagggtgct
      421 ggcttttcaa gttccttaag aatggcctca attttctcta tacactcagt tggaacacgc
      481 gacctgtcca ggttaagcac cattttatcg cccttataca atactgtcgc tccaggagca
      541 aactgatgtc gtgagcttaa actagttctt gatgcagatg acgttttaag cacagaagtt
      601 aaaagagtga taacttcttc agcttcaaat atcaccccag cttttttctg ctcatgaagg
      661 ttagatgcct gctgcttaag taattcctct ttatctgtaa aggctttttg aagtgcatca
      721 cctgaccggg cagatagttc accggggtga gaaaaaagag caacaactga tttaggcaat
      781 ttggcggtgt tgatacagcg ggtaataatc ttacgtgaaa tattttccgc atcagccagc
      841 gcagaaatat ttccagcaaa ttcattctgc aatcggcttg cataacgctg accacgttca
      901 taagcacttg ttgggcgata atcgttaccc aatctggata atgcagccat ctgctcatca
      961 tccagctcgc caaccagaac acgataatca ctttcggtaa gtgcagcagc tttacgacgg
     1021 cgactcccat cggcaatttc tatgacacca gatactcttc gaccgaacgc cggtgtctgt
     1081 tgaccagtca gtagaaaaga agggatgaga tcatccagtg cgtcctcagt aagcagctcc
     1141 tggtcacgtt cattacctga ccatacccga gaggtctttt caacactatc accccggagc
     1201 acttcaagag taaacttcac atcccgacca catacaggca aagtaatggc attaccgcga
     1261 gccattactc ctacgcgcgc aattaacgaa tccaccatcg gggcagctgg tgtcgataac
     1321 gaagtatctt caaccggttg agtattgagc gtatgttttg gaataacagg cgcacgcttc
     1381 attatctaat ctcccagcgt ggtttaatca gacgatcgaa aatttcattg cagacaggtt
     1441 cccaaataga aagagcattt ctccaggcac cagttgaaga gcgttgatca atggcctgtt
     1501 caaaaacagt tctcatccgg atctgacctt taccaacttc atccgtttca cgtacaacat
     1561 tttttagaac catgcttccc caggcatccc gaatttgctc ctccatccac ggggactgag
     1621 agccattact attgctgtat ttggtaagca aaatacgtac atcaggctcg aaccctttaa
     1681 gatcaacgtt cttgagcaga tcacgaagca tatcgaaaaa ctgcagtgcg gaggtgtagt
     1741 caaacaactc agcaggcgtg ggaacaatca gcacatcagc agcacatacg acattaatcg
     1801 tgccgatacc caggttaggc gcgctgtcaa taactatgac atcatagtca tgagcaacag
     1861 tttcaatggc cagtcggagc atcaggtgtg gatcggtggg cagtttacct tcatcaaatt
     1921 tgcccattaa ctcagtttca atacggtgca gagccagaca ggaaggaata atgtcaagcc
     1981 ccggccagca agtgggcttt attgcataag tgacatcgtc cttttcccca agatagaaag
     2041 gcaggagagt atcttctgca tgaatatgaa gatctggtac ccatccgtga tacattgagg
     2101 ctgttccctg ggggtcgtta ccttccacga gcaaaacacg tagccccttc agagccagat
     2161 cctgagcaag atgaacagaa actgaggttt tgtaaacgcc acctttatgg gcagcaaccc
     2221 cgatcaccgg tggaaataca tcttcagcac gtcgcaatcg cgtaccaaac acatcacgca
     2281 tatgattaat ttgttcaatt gtataaccaa cacgttgctc aacccgtcct cgaatttcca
     2341 tatccgggtg cggtagtcgc cctgctttct cggcatctct gatagcctga gaagaaaccc
     2401 caactaaatc cgctgcttca cctattctcc agcgccgggt tattttcctc gcttccgggc
     2461 tgtcatcatt aaactgtgca atggcgatag ccttcgtcat ttcatgacca gcgtttatgc
     2521 actggttaag tgtttccatg agtttcattc tgaacatcct ttaatcattg ctttgcgttt
     2581 ttttattaaa tcttgcaatt tactgcaaag caacaacaaa atcgcaaagt catcaaaaaa
     2641 ccgcaaagtt gtttaaaata agagcaacac tacaaaagga gataagaaga gcacatacct
     2701 cagtcactta ttatcactag cgctcgccgc agccgtgtaa ccgagcatag cgagcgaact
     2761 ggcgaggaag caaagaagaa ctgttctgtc agatagctct tacgctcagc gcaagaagaa
     2821 atatccaccg tgggaaaaac tccaggtaga ggtacacacg cggatagcca attcagagta
     2881 ataaactgtg ataatcaacc ctcatcaatg atgacgaact aacccccgat atcaggtcac
     2941 atgacgaagg gaaagagaag gaaatcaact gtgacaaact gccctcaaat ttggcttcct
     3001 taaaaattac agttcaaaaa gtatgagaaa atccatgcag gctgaaggaa acagcaaaac
     3061 tgtgacaaat taccctcagt aggtcagaac aaatgtgacg aaccaccctc aaatctgtga
     3121 cagataaccc tcagactatc ctgtcgtcat ggaagtgata tcgcggaagg aaaatacgat
     3181 atgagtcgtc tggcggcctt tctttttctc aatgtatgag aggcgcattg gagttctgct
     3241 gttgatctca ttaacacaga cctgcaggaa gcggcggcgg aagtcaggca tacgctggta
     3301 actttgaggc agctggtaac gctctatgat ccagtcgatt ttcagagaca cgatgcctga
     3361 gccatccggc ttacgatact gacacaggga ttcgtataaa cgcatggcat acggattggt
     3421 gatttctttt gtttcactaa gccgaaactg cgtaaaccgg ttctgtaacc cgataaagaa
     3481 gggaatgaga tatgggttga tatgtacact gtaaagccct ctggatggac tgtgcgcacg
     3541 tttgataaac caaggaaaag attcatagcc tttttcatcg ccggcatcct cttcagggcg
     3601 ataaaaaacc acttccttcc ccgcgaaact cttcaatgcc tgccgtatat ccttactggc
     3661 ttccgcagag gtcaatccga atatttcagc atatttagca acatggatct cgcagatacc
     3721 gtcatgttcc tgtagggtgc catcagattt tctgatctgg tcaacgaaca gatacagcat
     3781 acgtttttga tcccgggaga gactatatgc cgcctcagtg aggtcgtttg actggacgat
     3841 tcgcgggcta tttttacgtt tcttgtgatt gataaccgct gtttccgcca tgacagatcc
     3901 atgtgaagtg tgacaagttt ttagattgtc acactaaata aaaaagagtc aataagcagg
     3961 gataactttg tgaaaaaaca gcttcttctg agggcaattt gtcacagggt taagggcaat
     4021 ttgtcacaga caggactgtc atttgagggt gatttgtcac actgaaaggg caatttgtca
     4081 caacaccttc tctagaacca gcatggataa aggcctacaa ggcgctctaa aaaagaagat
     4141 ctaaaaacta taaaaaaaat aattataaaa atatccccgt ggataagtgg ataaccccaa
     4201 gggaagtttt ttcaggcatc gtgtgtaagc agaatatata agtgctgttc cctggtgctt
     4261 cctcgctcac tcgaaattcc cctttccgac gctcaccggg ctggttgccc tcgccgctgg
     4321 gctggcggcc gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag
     4381 acaccgcggc cggccgccgg cgttgtggat acctcgcgga aaacttggcc ctcactgaca
     4441 gatgaggggc ggacgttgac acttgagggg ccgactcacc cggcgcggcg ttgacagatg
     4501 aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc agcctcgcaa atctgcgaaa
     4561 acgcctgatt ttacgcgagt ttcccacaga tgatgtggac aagcctgggg ataagtgccc
     4621 tgcggtattg acacttgagg ggcgcgacta ctgacagatg aggggcgcga tccttgacac
     4681 ttgaggggca gagtgctgac agatgagggg cgcacctatt gacatttgag gggctgtcca
     4741 caggcagaaa atccagcatt tgcaagggtt tccgcccgtt tttcggccac cgctaacctg
     4801 tcttttaacc tgcttttaaa ccaatattta taaaccttgt ttttaaccag ggctgcgccc
     4861 tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc
     4921 gctaacgcgg gcctcccatc cccccagggg ctgcgcccct cggccgcgaa cggcctcacc
     4981 ccaaaaatgg caggggatct cgagggcttc gccctgtcgc tcgactgcgg cgagcactac
     5041 tggctgtaaa aggacagacc acatcatggt tctgtgttca ttaggttgtt ctgtccattg
     5101 ctgacataat ccgctccact tcaacgtaac accgcacgaa gatttctatt gttcctgaag
     5161 gcatattcaa atcgttttcg ttaccgcttg caggcatcat gacagaacac tacttcctat
     5221 aaacgctaca caggctcctg agattaataa tgcggatctc tacgataatg ggagattttc
     5281 ccgactgttt cgttcgcttc tcagtggata acagccagct tctctgttta acagacaaaa
     5341 acagcatatc cactcagttc cacatttcca tataaaggcc aaggcattta ttctcaggat
     5401 aattgtttca gcatcgcaac cgcatcagac tccggcatcg caaactgcac ccggtgccgg
     5461 gcagccacat ccagcgcaaa aaccttcgtg tagacttccg ttgaactgat ggacttatgt
     5521 cccatcaggc tttgcagaac tttcagcggt ataccggcat acagcatgtg catcgcatag
     5581 gaatggcgga acgtatgtgg tgtgaccgga acagagaacg tcacaccgtc agcagcagcg
     5641 gcggcaaccg cctccccaat ccaggtcctg accgttctgt ccgtcacttc ccagatccgc
     5701 gctttctctg tccttcctgt gcgacggtta cgccgctcca tgagcttatc gcgaataaat
     5761 acctgtgacg gaagatcact tcgcagaata aataaatcct ggtgtccctg ttgataccgg
     5821 gaagccctgg gccaactttt ggcgaaaatg agagtgagct gttggatggg gatcgcctga
     5881 atcgccccat catccagcca gaaagtgagg gagccacggt tgatgagagc tttgttgtag
     5941 gtggaccagt tggtgatttt gaacttttgc tttgccacgg aacggtctgc gttgtcggga
     6001 agatgcgtga tctgatcctt caactcagca aaagttcgat ttattcaaca aagccacgtt
     6061 gtgtctcaaa atctctggtg ttacattgca caagataaaa atatatcatc atgaacaata
     6121 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     6181 acgtcttgct caaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     6241 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct accgattgta tgggaagccc
     6301 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     6361 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     6421 atccgtactc ctgatgatgc atggttactc accactgcga tcccagggaa aacagcattc
     6481 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     6541 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     6601 cgactcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     6661 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     6721 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     6781 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     6841 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     6901 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     6961 gatgagtttt tctaatcact agaccaatgt tacacatata tactttagat tgatttaaaa
     7021 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgtgttga
     7081 attggtcgac gatgtaggtc actgtctcga agccgcggtg cgggtgccag ggcgtgccct
     7141 tgggctcccc gggcgcgtac tccacctcac ccatctggtc catcatgatg aacgggtcga
     7201 ggtggcggta gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg ccctcgaaac
     7261 cgctgggcgc ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg ggtgcggata
     7321 cgcggggcag cgtcagcggg ttctcgacgg tcacggcggg catgtcgatc acgatgagaa
     7381 tggccagacc atgatgaaat aacataaggt ggtcccgtcg gcaagagaca tccacttaac
     7441 gtatgcttgc aataagtgcg agtgaaagga atagtattct gagtgtcgta ttgagtctga
     7501 gtgagacagc gatatgattg ttgattaacc cttagcatgt ccgtggggtt tgaattaact
     7561 cataatatta attagacgaa attattttta aagttttatt tttaataatt tgcgagtacg
     7621 cataacaagc ttcgtctcag tcaggagaga gacccctgtt gacaattaat catcggcata
     7681 gtatatcggc atagtataat acgacaaggt gaggaactaa acccaggagg cagatcatga
     7741 ccatgattac gccaagctat ttaggtgaca ctatagaata ctcaagcttg catgcctgca
     7801 ggtcgactct agaggatccc cgggtaccga gctcgaattc gccctatagt gagtcgtatt
     7861 acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     7921 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     7981 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgacgcctg atgcggtatt
     8041 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     8101 gctctgatgc cgcatagggt ctctcgcttg agacgaagct tgacgctgct gctctaaacg
     8161 acgcatttcg tactccaaag tacgaatttt ttccctcaag ctcttatttt cattaaacaa
     8221 tgaacaggac ctaacgcaca gtcacgttat tgtttacata aatgattttt tttactattc
     8281 aaacttactc tgtttgtgta ctcccactgg tatagccttc ttttatcttt tctggttcag
     8341 gctctatcac tttactaggt acggcatctg cgttgagtcg cctcctttta aatgtctgac
     8401 cttttgcagg tgcagccttc cactgcgaat cattaaagtg ggtatcacaa atttgggagt
     8461 tttcaccaag gctgcaccca aggctctgct cccacaattt tctcttaata gcacacttcg
     8521 gcacgtgaat taattttact ccagtcacag ctttgcagca aaatttgcaa tatttcattt
     8581 ttttttattc cacgtaaggg ttaatgtttt caaaaaaaaa ttcgtccgca cacaaccttt
     8641 cctctcaaca agcaaacgtg cactgaattt aagtgtatac ttcggtaagc ttcggctatc
     8701 gacgggacca ccttatgtta tttcatcatg ggccagaccc acgtagtcca gactagtgga
     8761 tctcgacagc gacacacttg catcggatgc agcccggtta acgtgccggc acggcctggg
     8821 taaccaggta ttttgtccac ataaccgtgc gcaaaatgtt gtggataagc aggacacagc
     8881 agcaatccac agcaggcata caaccgcaca ccgaggttac tccgttctac aggttacgac
     8941 gacatgtcaa tacttgccct tgacaggcat tgatggaatc gtagtctcac gctgatagtc
     9001 tgatcgacaa ta
//
