LOCUS       sequence-33656-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-T2
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(89..112)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(287..306)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     556..574
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      704..713
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccgctgccgg tgctgccggt gccatgcccc aggaacaggt ggtggcggcc ctcggagcgc
       61 tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccag cttggtgtcc
      121 acgtagtagt agccgggcag ttgcacgggc ttcttggcca tgtagatggt cttgaactcc
      181 accaggtagt ggccgccgtc cttcagcttc agggcctggt ggatctcgcc cttcagcacg
      241 ccgtcgcggg ggtacaggcg ctcggtggag gcctcccagc ccatggtctt cttctgcatt
      301 acggggccgt cgggggggaa gttggtgccg cgcatcttca ccttgtagat cagcgtgccg
      361 tcctgcaggg aggagtcctg ggtcacggtc accagaccgc cgtcctcgaa gttcatcacg
      421 cgctcccact tgaagccctc ggggaaggac agcttcttgt aatcggggat gtcggcgggg
      481 tgcttcacgt acgccttgga gccgtacatg aactgggggg acaggatgtc ccaggcgaag
      541 ggcagggggc cgcccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg
      601 ccctcgccct cgccctcgat ctcgaactcg tggccgttca tggagccctc catgcgcacc
      661 ttgaagcgca tgaactcttt gatgacctcc tcgcccttgc tcaccatggt ggcgggatcc
      721 gggcgactca gtcaatcgga
//
