LOCUS       sequence-33695-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Zhao B, Li L, Tumaneng K, Wang CY, Guan KL
  TITLE     A coordinated phosphorylation by Lats and CK1 regulates YAP 
            stability through SCF(beta-TRCP).
  JOURNAL   Genes Dev. 2010 Jan 1. 24(1):72-85.
  PUBMED    20048001
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 Jan 1. 24(1):72-85."
COMMENT     Sequence Label: pCMV-Flag YAP S381A
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..59
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             93..116
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctcgtttagt gaaccgtcag aattgatcta ccatggacta caaagacgat gacgacaagc
       61 ttgcggccgc gaattcaagc ttagccacca tggactacaa agacgatgac gataaagcaa
      121 ggctcgaatc ggtacctaag gatcccgggc agcagccgcc gcctcaaccg gccccccagg
      181 gccaagggca gccgccttcg cagcccccgc aggggcaggg cccgccgtcc ggacccgggc
      241 aaccggcacc cgcggcgacc caggcggcgc cgcaggcacc ccccgccggg catcagatcg
      301 tgcacgtccg cggggactcg gagaccgacc tggaggcgct cttcaacgcc gtcatgaacc
      361 ccaagacggc caacgtgccc cagaccgtgc ccatgaggct ccggaagctg cccgactcct
      421 tcttcaagcc gccggagccc aaatcccact cccgacaggc cagtactgat gcaggcactg
      481 caggagccct gactccacag catgttcgag ctcattcctc tccagcttct ctgcagttgg
      541 gagctgtttc tcctgggaca ctgaccccca ctggagtagt ctctggccca gcagctacac
      601 ccacagctca gcatcttcga cagtcttctt ttgagatacc tgatgatgta cctctgccag
      661 caggttggga gatggcaaag acatcttctg gtcagagata cttcttaaat cacatcg
//
