LOCUS       sequence-33713-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B
  TITLE     Chaperone-based procedure to increase yields of soluble recombinant 
            proteins produced in E. coli.
  JOURNAL   BMC Biotechnol. 2007 Jun 12;7:32. doi: 10.1186/1472-6750-7-32.
  PUBMED    17565681
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-06-12"; volume: "7:32. doi"; pages: " 
            10.1186/1472-6750-7-32"
COMMENT     Sequence Label: pBB542
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     115..132
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(167..253)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(269..363)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc ttccaggaaa
       61 tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga gcgtagcgag
      121 tcagtgagcg aggaagcgga atatatccct aggtctaggg cggcggattt gtcctactca
      181 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct
      241 ttcgttttat ttgatgcctc tagcttggat tctcaccaat aaaaaacgcc cggcggcaac
      301 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
      361 gtccaagctc agctaattaa gcttacatca tgccgcccat gccacccatg ccgcccatac
      421 cgccagcagc gcctaagtca gctgcatcgt ttttcggcag gtcggtaacc atgcattcgg
      481 tggtgatcat caggccagcc acagaagctg cgtactgcag agcagaacga gttactttgg
      541 ttggatccag gatacccatg tcgatcatgt tgccgtattc ttcggttgct gcgttgtaac
      601 cgtagttgcc gtcgccgcct ttaacggtgt tagcaacaac agacggttct tcgccgcagt
      661 tcaatacgat ctgacgcagc ggagcttcca ttgcacgcag tgcaactttg atacccacgt
      721 tctggtcttc gttctgacca cgca
//
