LOCUS       sequence-337144-        4438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4438)
  TITLE     Xenopus Expression Plasmids
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: XE57 Delta N-cadherin
FEATURES             Location/Qualifiers
     source          1..4438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2092..2111)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2093..2111)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2198..2215)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2369..2957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2449..2468)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3128..3919,3920..3988))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3751..3770
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3989..4093)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4161..4179
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4217..4239)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4339..4358
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atttaggtga cactatagaa ctcgagcagt ccaagcctcc gccgacccca cagcatcacc
       61 atgtgccgga aacagccctt cctgctaccg actctactcg gcatcctagc ggccctgatg
      121 ctgcagcagg gaccagttga agcattcggg ggatcccttg attatggcat ctataatatt
      181 ccaattcaca tcacagattc gggaaatcct gccatgtcaa acacttcgta cctgcgtgtg
      241 aaggtctgct cttgtgagca tggatactgc agcactacag ctcccattat tggaactggc
      301 ttgggcacgg gagctattat tgcaattctt ctttgcatta tcattttact cactttagtg
      361 ttgatgtttg ttgtatggat gaagcgccgg gacaaggagc ggcaagcaaa gcagctactt
      421 attgatccag aagatgatgt cagagataac attctgaagt atgatgaaga aggtggtgga
      481 gaagaagatc aggactacga tttaagccag ttgcagcagc ctgacaccat ggaaccagac
      541 accattaaac ctgttggaat acgccgtatg gacgaaagac caatccatgc tgaatctcag
      601 tatcctatcc gctcagctgc tccccatcct ggtgatatag gagacttcat aaatgagggg
      661 ttgaaagcag cggacaatga cccaacagct ccaccttatg attccttgtt agtgtttgat
      721 tatgaaggca gcggctccac tgcggggtcg ctaagttcac tgaactcgtc cagtagtggc
      781 ggagagcaag actacgacta cttaaacgac tggggccctc gattcaagaa actcgccgat
      841 atgtatggtg gaagcgacga ctgaactttt gtttttggga caagaacaaa cattcgagct
      901 gatattccca aaaagcttaa ggaagcttgg ctttaacttt gtagtctact agcacaagtg
      961 cttgtttgaa ggctttggca taggctgcaa accaatttgg gctcagtggg aatatcagta
     1021 atccaatact gtttggagaa aaaaaaatga ttgagctcag atacacttga attttacagt
     1081 acagaagcac tgggatttta tgtgcctttt tgtacctttt tcagatcgga tttagtttta
     1141 tgttcaacgc tttaatggta ctgatttctg aaagggtgaa aagacaaggt acgttgcggg
     1201 tggggtgaca aaagtaaatc aagatatcct tcatcatgcc ttttctttac actgcattgc
     1261 tttttgttaa tatctgaaac agagaacaaa gttcattaga gtaactatta cattagttac
     1321 aatgaaacca gtagggtgac atgttcagtg cattattact agttttagat gttagggttt
     1381 tttttcattc aaacgctccg atttttgaag ttgattgcag ataaagcgag ttgtcatatc
     1441 actggtttaa tcacaatatt ttttttgttt ttcttttctg cacaagctag ggaaatgata
     1501 ggcacatttt ggtcttactg tacacttttt tcatttgggt acttttttca cttcattgta
     1561 aaaatgatat gtgtacataa tgttttatca gcatagtcta taaagaagtt gaactgcttc
     1621 aaacacgtgt atgtattatt tggactatgg attcaggttt tgcatgttta tatctttttc
     1681 tttattgata aagtatttac aaaacaggtt gaaacacaat tacgttcaat caaactgtag
     1741 attcagtaca caattttttt ttattggttt tgggggggcg ggtaataaaa tgttcttagc
     1801 acaaatgttt tacataattt gtaccaaata aaatacagat aggggtagcc tgacactggt
     1861 ggcactacta atacgagggg tgggaagtat ttagcctctt taggctaaag gacatctcac
     1921 tagttgcacc caagtaagat atgataacag ctttgactct gtattgtgta ccagactata
     1981 cattatgcac ctctgacccc aacattctga ataaaaatgc taatttttga aaaaaaaaaa
     2041 aaaaaaaaaa aaaaaaacgg aattcatcga tgatatcaga tctgccggtc tccctatagt
     2101 gagtcgtatt aatttcgata agccaggtta acctgcatta atgaatcggc caacgcgcgg
     2161 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2221 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2281 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2341 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2401 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2461 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2521 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2581 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2641 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2701 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2761 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2821 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2881 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2941 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3001 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3061 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3121 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3181 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3241 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3301 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3361 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3421 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3481 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3541 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3601 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3661 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3721 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3781 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3841 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3901 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3961 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4021 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4081 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     4141 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     4201 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     4261 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     4321 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatggaca
     4381 tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata cacatacg
//
