LOCUS       sequence-33723-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Zhao Y, Terry D, Shi L, Weinstein H, Blanchard SC, Javitch JA
  TITLE     Single-molecule dynamics of gating in a neurotransmitter transporter
            homologue.
  JOURNAL   Nature. 2010 May 13. 465(7295):188-93.
  PUBMED    20463731
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 May 13. 465(7295):188-93."
COMMENT     Sequence Label: pETO18G
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..55)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(59..76)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gggctttgtt agcagccgga tcctcgagtc aatgatggtg atgatggtga tgatgaccgc
       61 tgccgcgcgg caccagggtg cccgcgcttt catggttgcg gcggcgttcc gccagaaaca
      121 ccagaaaggt cagaaacaga aacaggccaa taatataaaa gcgggtaatc cacacggtcc
      181 aatgggtttc ttccataatt ttcggaatat attcccgggc ccacaccacc agcagcaccg
      241 ccagaaacgc cggggtaata tagcgcatca cataataata aatgcgcggc actttaataa
      301 tgccgccgcg gttaatctcc tcccacgctt tatccgcgcc aaaaatccaa aaaaaaataa
      361 tcagttcggt caggccaaaa aacaccacgc caatggtacc cgcccaaaaa tccatttcat
      421 ccaggctttt gttcagaaac atcaccagat gcgcgctaaa aaacacaatc gccgcggtcc
      481 acagcaccgc atgtttgcgg ctcagcttaa gttcatcttc cagaaacgca atcatcggct
      541 gcataatcgc aatgctgctg gtcaggcccg caaaaaacag cagaaaaaac cacagaaagc
      601 ccagaaaggt gccgcccgcg gtctggctaa aaatcgccgg cagggtaata aagcctaggt
      661 taaacgcgcc cgctttcgca atcgccaccg cgttcgccac gccaaaaaac gccaccgccg
      721 ccggaatgct aatgctgccg cccagaatca cttccgcttt ttcgttcang gtcgccgcgg
      781 tcaggccgct cagcacaata tcctgatctt tacgtacata gctcgcatag gtaataatcg
      841 cgccaaagcc caggctcagg gtaaaaaaaa tctggcccac cgccgcaatc cacacgcccg
      901 gatctttcag tttttcaaaa tccggggtcc acagaaagtt c
//
