LOCUS       sequence-337254-       10334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting of Vp64-dCas9 activation system to hTERT promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10334)
  AUTHORS   Penev A, Bazley A, Shen M, Boeke JD, Savage SA, Sfeir A
  TITLE     Alternative splicing is a developmental switch for hTERT expression.
  JOURNAL   Mol Cell. 2021 Apr 6. pii: S1097-2765(21)00228-8. doi: 
            10.1016/j.molcel.2021.03.033.
  PUBMED    33852895
REFERENCE   2  (bases 1 to 10334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Apr 6. pii: S1097-2765(21)00228-8. doi: 
            10.1016/j.molcel.2021.03.033."
COMMENT     Sequence Label: pLenti pTERT sgRNA2(MS2) mCherry
FEATURES             Location/Qualifiers
     source          1..10334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(80..100)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        85..414
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      265..400
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     327..346
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        462..509
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             528..902
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1032..1153
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1069..1088)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1123..1142
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1202..1218)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1202..1218)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1215..1237)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1226..1242
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1250..1256,1257..1274,1275..1280))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1295..1316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1433..1450)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1604..2192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1684..1703)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2363..3154,3155..3223))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2986..3005
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3224..3328)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3403..3422)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3594..3973
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3975..4173
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4128..4148
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4191..4371
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4418..4543
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5036..5269
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5454..5498
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5796..5913
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5963..6211
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5963..5983
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6134..6153
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        6250..6268
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        6320..6338
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        6462..7640
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6693..7631
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7588..7608
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7647..8357
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7792..7810)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8060..8079
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    8382..8970
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8435..8455)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8853..8864)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8973..8989)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8974..8990)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9495..9675
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9701..9718)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9707..9931
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9977..10334,1..71)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10064..10083)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10274..10295
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
       61 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
      121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
      181 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      241 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
      301 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
      361 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
      421 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
      481 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
      541 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
      601 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
      661 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
      721 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
      781 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
      841 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
      901 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
      961 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     1021 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     1081 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     1141 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     1201 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     1261 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     1321 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1381 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1441 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1501 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1561 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1621 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1681 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1741 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1801 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1861 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1921 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1981 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2041 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2101 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2161 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2221 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2281 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2341 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2401 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2461 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2521 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2581 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2641 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2701 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2761 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2821 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2881 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2941 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3001 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3061 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3121 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3181 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3241 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3301 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     3361 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     3421 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     3481 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     3541 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     3601 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     3661 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     3721 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     3781 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     3841 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     3901 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     3961 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     4021 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     4081 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     4141 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
     4201 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4261 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4321 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     4381 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     4441 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     4501 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     4561 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     4621 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     4681 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     4741 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     4801 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     4861 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     4921 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     4981 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     5041 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     5101 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     5161 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     5221 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     5281 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     5341 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     5401 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     5461 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     5521 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     5581 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     5641 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     5701 gtggagagag agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc
     5761 caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     5821 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     5881 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     5941 ccagtttggt taattagcta gcgagggcct atttcccatg attccttcat atttgcatat
     6001 acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt
     6061 agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt
     6121 atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
     6181 tttatatatc ttgtggaaag gacgaaacac cgccaggacc gcgcttccca cggttttaga
     6241 gctaggccaa catgaggatc acccatgtct gcagggccta gcaagttaaa ataaggctag
     6301 tccgttatca acttggccaa catgaggatc acccatgtct gcagggccaa gtggcaccga
     6361 gtcggtgctt tttttggatc ctgcaaagat ggataaagtt ttaaacagag aggaatcttt
     6421 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
     6481 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     6541 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     6601 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     6661 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     6721 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     6781 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     6841 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     6901 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     6961 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     7021 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     7081 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     7141 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     7201 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     7261 ggccgcttcc cggccctgct gcagggagct caaaatgaag gacgcggcgc tcgggagagc
     7321 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     7381 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     7441 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     7501 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     7561 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     7621 ttccatttca ggtgtcgtga tgtaccatgg tgagcaaggg cgaggaggat aacatggcca
     7681 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     7741 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     7801 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     7861 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     7921 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     7981 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     8041 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     8101 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     8161 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     8221 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     8281 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     8341 acgagctgta caagtgagaa ttcgatatca agcttatcgg taatcaacct ctggattaca
     8401 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     8461 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     8521 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     8581 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     8641 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     8701 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     8761 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     8821 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     8881 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     8941 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa
     9001 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     9061 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     9121 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     9181 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     9241 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
     9301 ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag
     9361 gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     9421 aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc
     9481 atccggactg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     9541 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     9601 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     9661 ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     9721 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     9781 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     9841 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     9901 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     9961 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    10021 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    10081 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    10141 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    10201 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    10261 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    10321 tattcttttg attt
//
