LOCUS       sequence-33738-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Blumenthal A, Trujillo C, Ehrt S, Schnappinger D
  TITLE     Simultaneous analysis of multiple Mycobacterium tuberculosis 
            knockdown mutants in vitro and in vivo.
  JOURNAL   PLoS One. 2010 Dec 22;5(12):e15667. doi: 
            10.1371/journal.pone.0015667.
  PUBMED    21203517
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-12-22"; volume: "5(12):e15667. doi"; pages: " 
            10.1371/journal.pone.0015667"
COMMENT     Sequence Label: pGMCSq17-10M-0X
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(24..48)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cttcgacgtt gtaaggttaa gccagcctgc ttttttgtac aaacttgtca gatctcgtaa
       61 tattggatcg tcgcaccggg ttaagcctgg agtgcggtgg tgcctggtcg gcattttcgc
      121 agtcgagggc tctcgtgtag cctgggcgag ttgccgacgc aggcgaccct cctgccacgg
      181 atccggtacc atcaggagga atcacttcgc aatgtcccgc ctggacaagt ccaaggtcat
      241 caactccgcc ctggagctgc tgaacgaggt cggcatcgag ggcctgacca cccgcaagct
      301 ggcccagaag ctgggcgtcg agcagccgac cctgtactgg cacgtcaaga acaagcgggc
      361 cctgctcgac gccctggcca tcgagatgtt ggaccgccac cacacccact tctgcccgct
      421 ggagggcgag agctggcagg acttcctgcg caacaacgcc aagagcttcc gctgcgcctt
      481 gctgagccac cgcgacggcg ccaaggtgca cctgggcacc cggccgaccg agaagcagta
      541 cgagaccctg gagaaccagt tggccttcct gtgccagcag ggcttctcgc tggagaacgc
      601 gttgtacgcc ctgtcggccg tgggccactt caccctgggc tgcgtgttgg aggaccagga
      661 gcaccaggtc gccaaggagg agcgcgagac cccgaccacc gacagcatgc cgccgttgct
      721 gcgccaggcc atcgagctgt tcgaccacca gggcgccgag ccggccttct tgttcggcct
      781 ggagctgatc atctgcggct tggagaagca gctgaagtgc gagagcggct cctaatgacc
      841 atggtgacct catgactc
//
