LOCUS       sequence-337389-        8626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   You J, Croyle JL, Nishimura A, Ozato K, Howley PM
  TITLE     Interaction of the bovine papillomavirus E2 protein with Brd4 
            tethers the viral DNA to host mitotic chromosomes.
  JOURNAL   Cell. 2004 Apr 30. 117(3):349-60.
  PUBMED    15109495
REFERENCE   2  (bases 1 to 8626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2004 
            Apr 30. 117(3):349-60."
COMMENT     Sequence Label: p5067 pOZN E2TR
FEATURES             Location/Qualifiers
     source          1..8626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..892
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             957..1373
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1303..1325
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1341..1357
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1383..1757
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1695..1711
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             1784..1807
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1820..1846
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             1820..1846
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2680..3229
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2824..2841)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3051..3070
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             4320..4789
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5485..5502)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5485..5501)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5494..5516)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5710..5729)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5829..5851
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5889..5907)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5975..6079
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6080..6148,6149..6940)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6298..6317)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7111..7699
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7600..7619
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7853..7870
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7987..8008
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8023..8028,8029..8046,8047..8053)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8061..8077
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8066..8088
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8085..8101
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8085..8101
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
      721 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
      781 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
      841 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
      901 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggcc
      961 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1021 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1081 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1141 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1201 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1261 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1321 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     1381 ggccatatga gatcctatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
     1441 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     1501 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     1561 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     1621 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     1681 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     1741 gaccatcctc tagactgcca gatcttccgc tcgatgaacc atggactaca aggacgacga
     1801 tgacaagctc gatggaggat acccctacga cgtgcccgac tacgccggag gactcgagat
     1861 ggccggtgct ggacgcattt actattctcg ctttggtgac gaggcagcca gatttagtac
     1921 aacagggcat tactctgtaa gagatcagga cagagtgtat gctggtgtct catccacctc
     1981 ttctgatttt agagatcgcc cagacggagt ctgggtcgca tccgaaggac ctgaaggaga
     2041 ccctgcagga aaagaagccg agccagccca gcctgtctct tctttgctcg gctcccccgc
     2101 ctgcggtccc atcagagcag gcctcggttg ggtacgggac ggtcctcgct cgcaccccta
     2161 caattttcct gcaggctcgg ggggctctat tctccgctct tcctccaccc cggtgcaggg
     2221 cacggtaccg gtggacttgg catcaaggca ggaagaagag gagcagtcgc ccgactccac
     2281 agaggaagaa ccagtgactc tcccaaggcg caccaccaat gatggattcc acctgttaaa
     2341 ggcaggaggg tcatgctttg ctctaatttc aggaactgct aaccaggtaa agtgctatcg
     2401 ctttcgggtg aaaaagaacc atagacatcg ctacgagaac tgcaccacca cctggttcac
     2461 agttgctgac aacggtgctg aaagacaagg acaagcacaa atactgatca cctttggatc
     2521 gccaagtcaa aggcaagact ttctgaaaca tgtaccacta cctcctggaa tgaacatttc
     2581 cggctttaca gccagcttgg acttctgagc ggccgcatcg agagatccgg gagacacaac
     2641 ggtttccctc tagcgggatc aattccgccc ccccccctaa cgttactggc cgaagccgct
     2701 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     2761 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     2821 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     2881 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     2941 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     3001 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     3061 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     3121 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     3181 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgataatac catggattca
     3241 tacctgctga tgtggggact gctcacgttc atcatggtgc ctggctgcca ggcagagctc
     3301 tgtgacgatg acccgccaga gatcccacac gccacattca aagccatggc ctacaaggaa
     3361 ggaaccatgt tgaactgtga atgcaagaga ggtttccgca gaataaaaag cgggtcactc
     3421 tatatgctct gtacaggaaa ctctagccac tcgtcctggg acaaccaatg tcaatgcaca
     3481 agctctgcca ctcggaacac aacgaaacaa gtgacacctc aacctgaaga acagaaagaa
     3541 aggaaaacca cagaaatgca aagtccaatg cagccagtgg accaagcgag ccttccaggt
     3601 cactgcaggg aacctccacc atgggaaaat gaagccacag agagaattta tcatttcgtg
     3661 gtggggcaga tggtttatta tcagtgcgtc cagggataca gggctctaca cagaggtcct
     3721 gctgagagcg tctgcaaaat gacccacggg aagacaaggt ggacccagcc ccagctcata
     3781 tgcacaggtg aaatggagac cagtcagttt ccaggtgaag agaagcctca ggcaagcccc
     3841 gaaggccgtc ctgagagtga gacttcctgc ctcgtcacaa caacagattt tcaaatacag
     3901 acagaaatgg ctgcaaccat ggagacgtcc atatttacaa cagagtacca ggtagcagtg
     3961 gccggctgtg ttttcctgct gatcagcgtc ctcctcctga gtgggctcac ctggcagcgg
     4021 agacagagga agagtagaag aacaatctag aggatccgga ttagtccaat ttgttaaaga
     4081 caggatatca gtggtccagg ctctagtttt gactcaacaa tatcaccagc tgaagcctat
     4141 agagtacgag ccatagataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     4201 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
     4261 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc
     4321 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     4381 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     4441 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga
     4501 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
     4561 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
     4621 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt
     4681 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
     4741 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg
     4801 tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca tagtacttaa
     4861 agttacattg gcttccttga aataaacatg gagtattcag aatgtgtcat aaatatttct
     4921 aattttaaga tagtatctcc attggctttc tactttttct tttatttttt tttgtcctct
     4981 gtcttccatt tgttgttgtt gttgtttgtt tgtttgtttg ttggttggtt ggttaatttt
     5041 tttttaaaga tcctacacta tagttcaagc tagactatta gctactctgt aacccagggt
     5101 gaccttgaag tcatgggtag cctgctgttt tagccttccc acatctaaga ttacaggtat
     5161 gagctatcat ttttggtata ttgattgatt gattgattga tgtgtgtgtg tgtgattgtg
     5221 tttgtgtgtg tgattgtgta tatgtgtgta tggttgtgtg tgattgtgtg tatgtatgtt
     5281 tgtgtgtgat tgtgtgtgtg tgattgtgca tgtgtgtgtg tgtgattgtg tttatgtgta
     5341 tgattgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg ttgtgtatat
     5401 atatttatgg tagtgagagg caacgctccg gctcaggtgt caggttggtt tttgagacag
     5461 agtctttcac ttagcttgga attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     5521 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     5581 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     5641 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     5701 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     5761 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     5821 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     5881 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     5941 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     6001 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     6061 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     6121 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     6181 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     6241 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     6301 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     6361 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     6421 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6481 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6541 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     6601 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     6661 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6721 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6781 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6841 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6901 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6961 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     7021 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7081 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7141 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7201 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     7261 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7321 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7381 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7441 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7501 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7561 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7621 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7681 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7741 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     7801 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     7861 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     7921 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     7981 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     8041 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     8101 ccatgattac gccaagcttt gctcttagga gtttcctaat acatcccaaa ctcaaatata
     8161 taaagcattt gacttgttct atgccctagg gggcgggggg aagctaagcc agcttttttt
     8221 aacatttaaa atgttaattc cattttaaat gcacagatgt ttttatttca taagggtttc
     8281 aatgtgcatg aatgctgcaa tattcctgtt accaaagcta gtataaataa aaatagataa
     8341 acgtggaaat tacttagagt ttctgtcatt aacgtttcct tcctcagttg acaacataaa
     8401 tgcgctgctg agaagccagt ttgcatctgt caggatcaat ttcccattat gccagtcata
     8461 ttaattacta gtcaattagt tgatttttat ttttgacata tacatgtgaa agaccccacc
     8521 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa
     8581 ctgagaatag aaaagttcag atcaaggtca ggaacagatg gaacag
//
