LOCUS       sequence-337527-        5569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express ExbD V47A, L49A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Kopp DR, Postle K
  TITLE     The Intrinsically Disordered Region of ExbD Is Required for Signal 
            Transduction.
  JOURNAL   J Bacteriol. 2020 Mar 11;202(7). pii: JB.00687-19. doi: 
            10.1128/JB.00687-19. Print 2020 Mar 11.
  PUBMED    31932309
REFERENCE   2  (bases 1 to 5569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol."; date: "2020-03-11"; volume: "202(7). pii: JB.00687-19. 
            doi"; pages: " 10.1128/JB.00687-19. Print 2020 Mar 11"
COMMENT     Sequence Label: pKP2201
FEATURES             Location/Qualifiers
     source          1..5569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1678..1697)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1835..1857)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2023..2040)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2194..2782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2274..2293)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      complement(2893..3348)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3030..3051)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3242..3261
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(join(3390..4181,4182..4250))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4013..4032
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4251..4342)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(4361..4388)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4480..4566)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4719..4736
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 atggcacatc caccacggct taatgacgac aaaccggtta tctggacggt atctgtaacg
       61 cgcctgttcg agctgtttcg cgatatcagc ctcgagtttg atcacctggc gaacattacc
      121 cctatccagc ttggctttga aaaagcggtg acctacatcc acaagaaact ggcaaacgaa
      181 cgctgtgacg ccatcatcgc cgctggatct aacggcgcgt acctgaaaag ccgcctgtca
      241 gtgccagtta ttttgattaa accgagcggc tacgatgtgt tacaggcact ggcaaaagcc
      301 ggaaaactca cctcttctat cggcgttgtc acttatcagg aaactattcc ggcactggtg
      361 gcgtttcaaa aaacctttaa tttgcgcctc gatcaacgta gctacattac cgaagaagac
      421 gcacgcgggc agattaacga gctaaaagct aacggcaccg aagcggtggt cggcgcgggg
      481 ctgattaccg atctggcaga agaagccgga atgaccggaa tttttatcta ttccgccgcc
      541 accgtgcgcc aggcgttcag cgatgcgctg gatatgacgc gcatgtcgtt acgccataac
      601 actcacgatg ccacccgcaa cgccctgaga actcgttacg tgctgggcga tatgctcggt
      661 caatcaccac agatggaaca agtacggcag actattttgc tgtatgcccg ctccagtgca
      721 gcggtgttga ttgaggggga aacggggacg ggcaaagagc tggcggccca ggcgattcat
      781 cgggaatatt ttgcccgcca cgatgcgcga cagggcaaaa agtcgcatcc gtttgttgca
      841 gtcaactgcg gggcgattgc cgaatcgctg ctggaagcag aactgtttgg ctatgaggaa
      901 ggggcgttta ccggctcgcg acgcggcggt cgcgccgggc tgtttgaaat cgcccacgga
      961 ggtacgctgt ttctcgatga gattggcgaa atgccgctgc cgttgcagac ccggctgctg
     1021 cgggtgctgg aagaaaaaga ggtcacccgc gtcggcgggc atcagcctgt tccggtggat
     1081 gtgcgggtca ttagcgccac tcactgcaat ctggaagaag atatgcggca agggcagttt
     1141 cgccgtgacc tgttttatcg gctgagtatt ttgcgtctgc aattgccacc actgcgcgag
     1201 cgggtggcgg atattctgcc actggcggaa agctttttga aagtgtctct ggcggcgctc
     1261 tccaccccgt tttctgccgc attacgccag ggattacagg caagcgaaac cgtgctggtg
     1321 cactacgact ggccgggcaa tattcgtgaa ctgcgcaata tgatggagcg actggcgcta
     1381 tttttaagtg tggaaccgac gccggattta acgccgcaat ttttgcagct gctactgccg
     1441 gaactggcgc gcgagtcggc gaaaactccc gctccacgct tactgacacc acaacaggca
     1501 ctggagaaat ttaatggcga taaaacagca gcggcgaatt atttaggcat cagccggacg
     1561 acgttctggc ggcggctgaa aagctgaatc gatgataagc tgtcaaacat gagcagatcc
     1621 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
     1681 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
     1741 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
     1801 tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     1861 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     1921 cgggtgttgg cgggtgtcgg ggcgcagcca tgaccctatg cggtgtgaaa taccgcacag
     1981 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     2041 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2101 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2161 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2221 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2281 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2341 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2401 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2461 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2521 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2581 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     2641 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2701 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2761 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2821 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2881 ctagatcctt ttaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt
     2941 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     3001 aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     3061 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     3121 aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     3181 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     3241 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     3301 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtaa atcaatctaa
     3361 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3421 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3481 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3541 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3601 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3661 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3721 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3781 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3841 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3901 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3961 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
     4021 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4081 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4141 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4201 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4261 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4321 tgtatttaga aaaataaaca aaagagtttg tagaaacgca aaaaggccat ccgtcaggat
     4381 ggccttctgc ttaatttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
     4441 gggccgttgc ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
     4501 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
     4561 tatttgatgc ctggcagttc cctactctcg catggggaga ccccacacta ccatcggcgc
     4621 tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg ctactgccgc
     4681 caggcaaatt ctgttttatc agaccgcttc tgcgttctga tttaatctgt atcaggctga
     4741 aaatcttctc tcatccgcca aaacagccaa gcttgcatgc ctgcaggtcg actctagagg
     4801 atccccgggt accatggtta cttcgctttg gcggtttctt cgccgaccag acctatcttc
     4861 aggtaacccg cctgatgcag cgtatccatt accttcatca acgtctcgta atcgacggtt
     4921 ttgtccgctc ggaagaagat ggtggtgtct ttcttgcctt cggttaacgc attcaacgcc
     4981 gtaatcattg tttcatcggt gaccggatcg ttaccgataa acatcgagtt gtctgccttc
     5041 accgacagat aaaccggttt ttccgggcgc ggctgcggcg tgctggtaga agcaggtgcg
     5101 tttgccttca catctaccgt cgctaacggt gccgccacca taaagataat cagcagaacc
     5161 aacatcacgt cgataaacgg cgtcacgttg atatcatgca tttcgccgtt atcgtcgagg
     5221 ttttcgttaa gatgcattgc catcggatat tatcctgcgc gtaatttttg tgcgacacga
     5281 accggatgcg cagcggcgct ggcttccaga tccaggtcca tggtgaattc ctcctgctag
     5341 cttgttatca acttgttatt tgcgttgata aagacaaagc aaggggtgtg ccacaattgc
     5401 cttaataaaa ttaaatgttt aatttcaatt tgttagagat aaagtcatga actaatccgc
     5461 aaatatgcgt ttcagttaac gtttcaggca atgtttcacc gcgtttcatt gcaacaatta
     5521 tgaaacaaga ctaaacccaa tattcggttt cttaactttg cggtgcgct
//
