LOCUS       sequence-33754-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Shechner DM, Grant RA, Bagby SC, Koldobskaya Y, Piccirilli JA, 
            Bartel DP
  TITLE     Crystal structure of the catalytic core of an RNA-polymerase 
            ribozyme.
  JOURNAL   Science. 2009 Nov 27. 326(5957):1271-5.
  PUBMED    19965478
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2009 Nov 27. 326(5957):1271-5."
COMMENT     Sequence Label: p307HU
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(202..219)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(202..218)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(211..233)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(427..446)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     546..568
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(606..624)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        692..796
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tctgcccgaa gggcttgaga acatacccat tgcactccgg gtatgcagag gtggcagcct
       61 ccggtgggtt aaaacccaac gttctcaaca atagtgaggc cggcatggtc ccagcctcct
      121 cgctggcgcc ggctgggcaa cattccgagg ggaccgtccc ctcggtaatg gcgaatggga
      181 cccacaaatg aagagggatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      241 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      301 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
      361 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
      421 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
      481 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
      541 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
      601 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      661 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      721 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      781 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      841 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      901 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      961 tgagagtttt cgcccc
//
