LOCUS       sequence-33803-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Johnson BS, Snead D, Lee JJ, McCaffery JM, Shorter J, Gitler AD
  TITLE     TDP-43 is intrinsically aggregation-prone, and amyotrophic lateral 
            sclerosis-linked mutations accelerate aggregation and increase 
            toxicity.
  JOURNAL   J Biol Chem. 2009 Jul 24. 284(30):20329-39.
  PUBMED    19465477
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2009 Jul 24. 284(30):20329-39."
COMMENT     Sequence Label: pDuet TDP-43 M337V
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..57)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    166..190
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(190..209)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(191..209)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctgcgctgta gacgagtcca tgtgctggcg ttcaaatttc gcagcagcgg tttctttacc
       61 agactcgagg gtaccgacgt cagcgatcgc gtggccggcc gatatccaat tgagatctgc
      121 catatgtata tctccttctt atacttaact aatatactaa gatggggaat tgttatccgc
      181 tcacaattcc cctatagtga gtcgtattaa tttcgattat gcggccgtgt acaatacgat
      241 tactttctgt tcgacttaag cattatgcgg ccgcaagctt ctacattccc cagccagaag
      301 acttagaatc catgcttgag ccaaagcctc cattaaaacc actgcccgac cctgcattgg
      361 atgctgatcc ccaaccaatt gctgcaccag aattagagcc actataagag ttatttccag
      421 aaccgaaggc ctggtttggc tccctctgca tgttgccttg gttttggtta ttacccgatg
      481 ggcctgactg gttctgctgg ctggctaaca tgcccaccat accccaactg ctctgtagtg
      541 ctgcctgggc ggcagccatc atggctggat taatgctgaa cgcaccaaag ttcatcccac
      601 cacccatatt actaccttga ttgtttccca aaccagctcc accccctctg ctattaccaa
      661 atccaccctg attcccaaag ccacctggat taccaccaaa tcttccactt ctttctaact
      721 gtctattgct attgtgctta ggttcggcat tggatatatg aacgctgatt cctttaatga
      781 tcaagtcctc tccacaaaga gactgcgcaa tctgatcatc tgcaaatgta acaaaggcaa
      841 aggccctgaa tggcttgggg atgaagacat ccatcacatc cccgtactg
//
