LOCUS       sequence-338389-        2343 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses D. melanogaster Drp1 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Kamerkar SC, Kraus F, Sharpe AJ, Pucadyil TJ, Ryan MT
  TITLE     Dynamin-related protein 1 has membrane constricting and severing 
            abilities sufficient for mitochondrial and peroxisomal fission.
  JOURNAL   Nat Commun. 2018 Dec 7;9(1):5239. doi: 10.1038/s41467-018-07543-w.
  PUBMED    30531964
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-7"; volume: "9(1):5239. doi"; pages: " 
            10.1038/s41467-018-07543-w"
COMMENT     Sequence Label: 6xHis(TEV)-dDrp1-StrepII
FEATURES             Location/Qualifiers
     source          1..2343
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             65..85
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2297..2320
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca
       61 tcacgaaaat ctgtatttcc aaggctcgat ggaggcacta attccggtca taaacaagct
      121 gcaggacgtc ttcaacaccg tgggctccga ctccatccaa ttgccccaaa ttgttgttct
      181 aggcagtcag agctccggca agagttcggt gatcgagagc gttgtgggac gatcctttct
      241 gccccgtgga actggtattg tgacccgtcg cccactggtg ctccagctga tctacagccc
      301 actcgatgat cgcgagaatc gctcggcgga aaatggtacg tccaatgccg aggagtgggg
      361 ccgcttcctg cacaccaaga agtgcttcac ggactttgac gagatccgca aggagatcga
      421 aaacgaaacg gagcgggcgg cgggcagcaa caagggcatc tgcccggagc ccatcaacct
      481 gaagatattc tcgacgcacg tggtcaacct aacgctagtg gatctgcccg gcatcaccaa
      541 ggtcccggtg ggcgatcagc cggaggatat cgaggcgcag atcaaagaat tggtcctcaa
      601 gtacattgag aacccgaact ccatcatttt ggctgtgacg gccgccaaca cggacatggc
      661 caccagtgag gcactcaagc tggccaagga cgtggacccc gatggccggc gcactctggc
      721 cgtggtcacg aaactggatc tcatggatgc cggcaccgat gccattgaca tcctgtgcgg
      781 ccgggtcatt cccgttaagc tgggcatcat cggcgtgatg aatcgctcgc agaaggacat
      841 catggaccaa aagcacatcg acgatcagat gaaggacgag gccgccttcc tgcagcgcaa
      901 gtatcccact ctggccaccc ggaacggcac gccctacttg gccaagaccc tcaaccggct
      961 cctgatgcac cacatccggg actgcctgcc agatcttaag actcgcgtca acatcatggc
     1021 cacgcaattc caatcgctgc taaactccta tggcgaggat gtctccgaca agagccagac
     1081 cctgctgcag atcatcacga agttctccag cgcctactgc tgcacaatcg agggcacggc
     1141 caggaatatc gagaccacgg aactgtgcgg cggtgcccgg atgggctaca ttttccacga
     1201 gaccttcggc cgcaccctgg actccattca cccgctggcg ggtctgagca agatggacat
     1261 actgacagcc atccggaatg ccactggtcc acgcccggct ttgttcgttc cggaggtctc
     1321 gttcgaactg ctagtgaagc gccagattcg ccgcctggag gaaccctcct tgcgctgcgt
     1381 ggaactcatt cacgaggaga tgcagcgcat tgttcagcat tgtggcaacg aagtgcagca
     1441 ggagatgctg cgcttcccca aactgcacga gaagattgtg gacgtggtca cccagctgct
     1501 gcgtcgccgt ctgccgcaca ccaacgtgat ggtggagaac attgtggcca tcgagctggc
     1561 ctacatcaac accaagcacc ccgacttcca caaggatgcc gctttggtgc ccagtctgct
     1621 gaaaacggac agcgatccct actcacagat aaatctgggc cagcggcggg ccaatacacc
     1681 gcgcaaccac atgtcgcctc agatttcgtc gcacagtgct ggttcacagc agccgcagca
     1741 gcagcagccg ccgcagccga gttcctcaca gcagcaatat agccaagtgc atgagcagaa
     1801 tcacgtcgcc gagaactcga cgccttcgat ggcgagcaca tggctgtcga acattttacc
     1861 gccggcacca acgcgtccgg acagcattga gaactcgacc aacaacactc cggttcacaa
     1921 taacatcgtg agtccagtga agccggtcaa tctgctgccc gatgtgccgg ccaaccacaa
     1981 tccacgtcgc ctcactgaca aggagcagaa ggattgcgat gtcatcgaac acctgatcaa
     2041 atcgtatttc tacattgtgc gcaaatccat acaagactcg gttccaaagg ccatcatgca
     2101 tttcttggtt aactacgtta aggacaactt gcagagcgaa ttggttacac atctgtataa
     2161 gtcggataag gctgaaaccc tgctcaacga gtccgatcac attgcagtgc gccgaaaaga
     2221 ggcggccgat atgctgaagg cactgacgcg ggcgaatcac atcatcagcg aaatccgcga
     2281 aactcacatg tggctatggt ctcatcctca gttcgaaaaa taagatccgg ctgctaacaa
     2341 agc
//
