LOCUS       sequence-338717-        4028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Streptomyces codon-optimised beta-D-glucuronidase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Moore SJ, Lai HE, Chee SM, Toh M, Coode S, Chengan K, Capel P, Corre
            C, de Los Santos EL, Freemont PS
  TITLE     A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2021 Feb 19;10(2):402-411. doi: 
            10.1021/acssynbio.0c00581. Epub 2021 Jan 26.
  PUBMED    33497199
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00581"; journalName: "ACS Synth Biol"; date: 
            "2021-02-19- 19"; volume: "10"; issue: "2"; pages: "402-411"
COMMENT     Sequence Label: pTU1-B-SP44s-GUS
FEATURES             Location/Qualifiers
     source          1..4028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1976..1993)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2147..2735)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2227..2246)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2906..3697,3698..3766))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3529..3548
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3767..3871)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3939..3957
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3961..4004)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    4007..4026
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcatgc cctatttgac aacatgctgt gcggtgttgt aaagtgtact ttaactttaa
       61 gaaggagata tacatatgct gcgcccggtc gaaaccccga cgcgcgagat caagaagctc
      121 gacggcctct gggccttctc gctcgaccgc gagaactgcg gcatcgacca gcggtggtgg
      181 gagagcgccc tccaggagtc ccgcgcgatc gccgtgccgg gctccttcaa cgaccagttc
      241 gccgacgccg acatccgcaa ctacgccggc aacgtgtggt accagcgcga ggtgttcatc
      301 ccgaagggct gggccggcca gcgcatcgtc ctccgcttcg acgcggtcac ccactacggc
      361 aaggtctggg tcaacaacca ggaggtcatg gagcaccagg gcggctacac cccgttcgag
      421 gccgacgtca ccccgtacgt gatcgccggc aagtccgtcc gcatcaccgt ctgcgtgaac
      481 aacgagctga actggcagac gatcccgccg ggcatggtca tcaccgacga gaacggcaag
      541 aagaagcagt cctacttcca cgacttcttc aactacgcgg gcatccaccg ctccgtcatg
      601 ctctacacca cgccgaacac ctgggtcgac gacatcacgg tcgtcaccca cgtcgcccag
      661 gactgcaacc acgcctccgt cgactggcag gtcgtcgcca acggcgacgt cagcgtcgag
      721 ctgcgggacg ccgaccagca ggtcgtggcg accggccagg gcacctcggg caccctccag
      781 gtcgtcaacc cgcacctctg gcagcccggc gagggctacc tctacgagct gtgcgtcacc
      841 gccaagtcgc agaccgagtg cgacatctac ccgctccgcg tcggcatccg gtccgtcgcc
      901 gtcaagggcg agcagttcct catcaaccac aagccgttct acttcaccgg cttcggccgc
      961 cacgaggacg cggacctccg cggcaagggc ttcgacaacg tcctcatggt ccacgaccac
     1021 gcgctcatgg actggatcgg cgccaactcc taccgcacct cgcactaccc gtacgccgag
     1081 gagatgctcg actgggccga cgagcacggc atcgtcgtca tcgacgaaac cgccgccgtc
     1141 ggcttcaacc tctcgctcgg catcggcttc gaggcgggca acaagccgaa ggagctgtac
     1201 tccgaggagg cggtcaacgg cgaaacccag caggcccacc tccaggccat caaggagctg
     1261 atcgcccgcg acaagaacca cccgtccgtc gtcatgtggt cgatcgcgaa cgagccggac
     1321 acgcgcccgc agggcgcccg cgagtacttc gccccgctcg ccgaggcgac ccgcaagctg
     1381 gacccgacgc ggccgatcac gtgcgtcaac gtcatgttct gcgacgccca caccgacacc
     1441 atctccgacc tcttcgacgt cctctgcctc aaccgctact acggctggta cgtccagtcc
     1501 ggcgacctcg aaaccgcgga gaaggtcctc gagaaggagc tgctggcctg gcaggagaag
     1561 ctgcaccagc cgatcatcat caccgagtac ggcgtcgaca ccctcgcggg cctccactcc
     1621 atgtacaccg acatgtggtc cgaggagtac cagtgcgcct ggctcgacat gtaccaccgc
     1681 gtgttcgacc gcgtcagcgc cgtcgtcggc gagcaggtct ggaacttcgc cgacttcgcc
     1741 acctcgcagg gcatcctgcg cgtcggcggc aacaagaagg gcatcttcac ccgcgaccgg
     1801 aagccgaagt ccgccgcctt cctcctccag aagcgctgga ccggcatgaa cttcggcgag
     1861 aagccgcagc agggcggcaa gcagtgagga tcctcgaaaa aaaaaaaaag gcctcccaaa
     1921 tcggggggcc ttttttattg ataacaaaat gttccggaga gacgctgcag gcttcctcgc
     1981 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2041 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2101 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc
     2161 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2221 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2281 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2341 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2401 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2461 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2521 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2581 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2641 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2701 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2761 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2821 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     2881 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     2941 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3001 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagag ccacgctcac
     3061 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3121 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3181 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3241 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3301 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3361 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3421 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     3481 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     3541 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3601 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3661 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     3721 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     3781 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     3841 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     3901 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
     3961 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
     4021 ttctagaa
//
