LOCUS       sequence-338842-        6607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAPTURE method BAC origin of replication receiver plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Enghiad B, Huang C, Guo F, Jiang G, Wang B, Tabatabaei SK, Martin 
            TA, Zhao H
  TITLE     Cas12a-assisted precise targeted cloning using in vivo Cre-lox 
            recombination.
  JOURNAL   Nat Commun. 2021 Feb 19;12(1):1171. doi: 10.1038/s41467-021-21275-4.
  PUBMED    33608525
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-02-19"; volume: "12(1):1171. doi"; pages: " 
            10.1038/s41467-021-21275-4"
COMMENT     Sequence Label: pBE48
FEATURES             Location/Qualifiers
     source          1..6607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(74..865,866..934))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     697..716
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(935..1039)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    complement(1062..1095)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      2012..2231
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             2322..3077
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    3080..3330
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             3656..4831
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             4831..5802
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    5875..6348
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
ORIGIN
        1 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
       61 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      121 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      181 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
      241 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
      301 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
      361 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
      421 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
      481 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
      541 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
      601 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
      661 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
      721 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
      781 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
      841 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
      901 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
      961 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1021 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc aataacttcg tatagcatac
     1081 attatacgaa gttatttcgc caaaagttgg cccagggctt cccggtatca acagggacac
     1141 caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg cgataagctc
     1201 atggagcggc gtaaccgtcg cacaggaagg acagagaaag cgcggatctg ggaagtgacg
     1261 gacagaacgg tcaggacctg gattggggag gcggttgccg ccgctgctgc tgacggtgtg
     1321 acgttctctg ttccggtcac accacatacg ttccgccatt cctatgcgat gcacatgctg
     1381 tatgccggta taccgctgaa agttctgcaa agcctgatgg gacataagtc catcagttca
     1441 acggaagtct acacgaaggt ttttgcgctg gatgtggctg cccggcaccg ggtgcagttt
     1501 gcgatgccgg agtctgatgc ggttgcgatg ctgaaacaat tatcctgaga ataaatgcct
     1561 tggcctttat atggaaatgt ggaactgagt ggatatgctg tttttgtctg ttaaacagag
     1621 aagctggctg ttatccactg agaagcgaac gaaacagtcg ggaaaatctc ccattatcgt
     1681 agagatccgc attattaatc tcaggagcct gtgtagcgtt tataggaagt agtgttctgt
     1741 catgatgcct gcaagcggta acgaaaacga tttgaatatg ccttcaggaa caatagaaat
     1801 cttcgtgcgg tgttacgttg aagtggagcg gattatgtca gcaatggaca gaacaaccta
     1861 atgaacacag aaccatgatg tggtctgtcc ttttacagcc agtagtgctc gccgcagtcg
     1921 agcgacaggg cgaagccctc gagtgagcga ggaagcacca gggaacagca cttatatatt
     1981 ctgcttacac acgatgcctg aaaaaacttc ccttggggtt atccacttat ccacggggat
     2041 atttttataa ttattttttt tatagttttt agatcttctt ttttagagcg ccttgtaggc
     2101 ctttatccat gctggttcta gagaaggtgt tgtgacaaat tgccctttca gtgtgacaaa
     2161 tcaccctcaa atgacagtcc tgtctgtgac aaattgccct taaccctgtg acaaattgcc
     2221 ctcagaagaa gctgtttttt cacaaagtta tccctgctta ttgactcttt tttatttagt
     2281 gtgacaatct aaaaacttgt cacacttcac atggatctgt catggcggaa acagcggtta
     2341 tcaatcacaa gaaacgtaaa aatagcccgc gaatcgtcca gtcaaacgac ctcactgagg
     2401 cggcatatag tctctcccgg gatcaaaaac gtatgctgta tctgttcgtt gaccagatca
     2461 gaaaatctga tggcacccta caggaacatg acggtatctg cgagatccat gttgctaaat
     2521 atgctgaaat attcggattg acctctgcgg aagccagtaa ggatatacgg caggcattga
     2581 agagtttcgc ggggaaggaa gtggtttttt atcgccctga agaggatgcc ggcgatgaaa
     2641 aaggctatga atcttttcct tggtttatca aacgtgcgca cagtccatcc agagggcttt
     2701 acagtgtaca tatcaaccca tatctcattc ccttctttat cgggttacag aaccggttta
     2761 cgcagtttcg gcttagtgaa acaaaagaaa tcaccaatcc gtatgccatg cgtttatacg
     2821 aatccctgtg tcagtatcgt aagccggatg gctcaggcat cgtctctctg aaaatcgact
     2881 ggatcataga gcgttaccag ctgcctcaaa gttaccagcg tatgcctgac ttccgccgcc
     2941 gcttcctgca ggtctgtgtt aatgagatca acagcagaac tccaatgcgc ctctcataca
     3001 ttgagaaaaa gaaaggccgc cagacgactc atatcgtatt ttccttccgc gatatcactt
     3061 ccatgacgac aggatagtct gagggttatc tgtcacagat ttgagggtgg ttcgtcacat
     3121 ttgttctgac ctactgaggg taatttgtca cagttttgct gtttccttca gcctgcatgg
     3181 attttctcat actttttgaa ctgtaatttt taaggaagcc aaatttgagg gcagtttgtc
     3241 acagttgatt tccttctctt tcccttcgtc atgtgacctg atatcggggg ttagttcgtc
     3301 atcattgatg agggttgatt atcacagttt attactctga attggctatc cgcgtgtgta
     3361 cctctacctg gagtttttcc cacggtggat atttcttctt gcgctgagcg taagagctat
     3421 ctgacagaac agttcttctt tgcttcctcg ccagttcgct cgctatgctc ggttacacgg
     3481 ctgcggcgag cgctagtgat aataagtgac tgaggtatgt gctcttctta tctccttttg
     3541 tagtgttgct cttattttaa acaactttgc ggttttttga tgactttgcg attttgttgt
     3601 tgctttgcag taaattgcaa gatttaataa aaaaacgcaa agcaatgatt aaaggatgtt
     3661 cagaatgaaa ctcatggaaa cacttaacca gtgcataaac gctggtcatg aaatgacgaa
     3721 ggctatcgcc attgcacagt ttaatgatga cagcccggaa gcgaggaaaa taacccggcg
     3781 ctggagaata ggtgaagcag cggatttagt tggggtttct tctcaggcta tcagagatgc
     3841 cgagaaagca gggcgactac cgcacccgga tatggaaatt cgaggacggg ttgagcaacg
     3901 tgttggttat acaattgaac aaattaatca tatgcgtgat gtgtttggta cgcgattgcg
     3961 acgtgctgaa gacgtatttc caccggtgat cggggttgct gcccataaag gtggcgttta
     4021 caaaacctca gtttctgttc atcttgctca ggatctggct ctgaaggggc tacgtgtttt
     4081 gctcgtggaa ggtaacgacc cccagggaac agcctcaatg tatcacggat gggtaccaga
     4141 tcttcatatt catgcagaag acactctcct gcctttctat cttggggaaa aggacgatgt
     4201 cacttatgca ataaagccca cttgctggcc ggggcttgac attattcctt cctgtctggc
     4261 tctgcaccgt attgaaactg agttaatggg caaatttgat gaaggtaaac tgcccaccga
     4321 tccacacctg atgctccgac tggccattga aactgttgct catgactatg atgtcatagt
     4381 tattgacagc gcgcctaacc tgggtatcgg cacgattaat gtcgtatgtg ctgctgatgt
     4441 gctgattgtt cccacgcctg ctgagttgtt tgactacacc tccgcactgc agtttttcga
     4501 tatgcttcgt gatctgctca agaacgttga tcttaaaggg ttcgagcctg atgtacgtat
     4561 tttgcttacc aaatacagca atagtaatgg ctctcagtcc ccgtggatgg aggagcaaat
     4621 tcgggatgcc tggggaagca tggttctaaa aaatgttgta cgtgaaacgg atgaagttgg
     4681 taaaggtcag atccggatga gaactgtttt tgaacaggcc attgatcaac gctcttcaac
     4741 tggtgcctgg agaaatgctc tttctatttg ggaacctgtc tgcaatgaaa ttttcgatcg
     4801 tctgattaaa ccacgctggg agattagata atgaagcgtg cgcctgttat tccaaaacat
     4861 acgctcaata ctcaaccggt tgaagatact tcgttatcga caccagctgc cccgatggtg
     4921 gattcgttaa ttgcgcgcgt aggagtaatg gctcgcggta atgccattac tttgcctgta
     4981 tgtggtcggg atgtgaagtt tactcttgaa gtgctccggg gtgatagtgt tgagaagacc
     5041 tctcgggtat ggtcaggtaa tgaacgtgac caggagctgc ttactgagga cgcactggat
     5101 gatctcatcc cttcttttct actgactggt caacagacac cggcgttcgg tcgaagagta
     5161 tctggtgtca tagaaattgc cgatgggagt cgccgtcgta aagctgctgc acttaccgaa
     5221 agtgattatc gtgttctggt tggcgagctg gatgatgagc agatggctgc attatccaga
     5281 ttgggtaacg attatcgccc aacaagtgct tatgaacgtg gtcagcgtta tgcaagccga
     5341 ttgcagaatg aatttgctgg aaatatttct gcgctggctg atgcggaaaa tatttcacgt
     5401 aagattatta cccgctgtat caacaccgcc aaattgccta aatcagttgt tgctcttttt
     5461 tctcaccccg gtgaactatc tgcccggtca ggtgatgcac ttcaaaaagc ctttacagat
     5521 aaagaggaat tacttaagca gcaggcatct aaccttcatg agcagaaaaa agctggggtg
     5581 atatttgaag ctgaagaagt tatcactctt ttaacttctg tgcttaaaac gtcatctgca
     5641 tcaagaacta gtttaagctc acgacatcag tttgctcctg gagcgacagt attgtataag
     5701 ggcgataaaa tggtgcttaa cctggacagg tctcgtgttc caactgagtg tatagagaaa
     5761 attgaggcca ttcttaagga acttgaaaag ccagcaccct gatgcgacca cgttttagtc
     5821 tacgtttatc tgtctttact taatgtcctt tgttacaggc cagaaagcat aactggcctg
     5881 aatattctct ctgggcccac tgttccactt gtatcgtcgg tctgataatc agactgggac
     5941 cacggtccca ctcgtatcgt cggtctgatt attagtctgg gaccacggtc ccactcgtat
     6001 cgtcggtctg attattagtc tgggaccacg gtcccactcg tatcgtcggt ctgataatca
     6061 gactgggacc acggtcccac tcgtatcgtc ggtctgatta ttagtctggg accatggtcc
     6121 cactcgtatc gtcggtctga ttattagtct gggaccacgg tcccactcgt atcgtcggtc
     6181 tgattattag tctggaacca cggtcccact cgtatcgtcg gtctgattat tagtctggga
     6241 ccacggtccc actcgtatcg tcggtctgat tattagtctg ggaccacgat cccactcgtg
     6301 ttgtcggtct gattatcggt ctgggaccac ggtcccactt gtattgtcga tcagactatc
     6361 agcgtgagac tacgattcca tcaatgcctg tcaagggcaa gtattgacat gtcgtcgtaa
     6421 cctgtagaac ggagtaacct cggtgtgcgg ttgtatgcct gctgtggatt gctgctgtgt
     6481 cctgcttatc cacaacattt tgcgcacggt tatgtggaca aaatacctgg ttacccaggc
     6541 cgtgccggca cgttaaccgg gctgcatccg atgcaagtgt gtcgctgtcg acgagctcgc
     6601 gagctcg
//
