LOCUS       sequence-338890-        9294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of a truncated version of hNFAT (aa 2-409) 
            fused to the DNA-binding domain from TALEN1257, the VP64 activation 
            domain and the membrane anchor peptide KRɸ..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Mesko M, Lebar T, Dekleva P, Jerala R, Bencina M
  TITLE     Engineering and Rewiring of a Calcium-Dependent Signaling Pathway.
  JOURNAL   ACS Synth Biol. 2020 Aug 21;9(8):2055-2065. doi: 
            10.1021/acssynbio.0c00133. Epub 2020 Jul 20.
  PUBMED    32643923
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00133"; journalName: "ACS Synth Biol"; date: 
            "2020-08-21- 21"; volume: "9"; issue: "8"; pages: "2055-2065"
COMMENT     Sequence Label: hNFAT2-409:TALE:VP64:KRɸ
FEATURES             Location/Qualifiers
     source          1..9294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        107..486
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        487..690
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     641..661
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     735..754
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        735..753
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      816..825
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             825..854
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4458..4607
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        complement(4719..4737)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4720..4737)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4757..4774)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4763..4987
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5033..5461
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5120..5139)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5330..5351
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5470..5490)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5475..5804
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5655..5790
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5717..5736
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5871..6665
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5925..5944)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6535..6554
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6839..6960
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6876..6895)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6930..6949
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7009..7025)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7009..7025)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7022..7044)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7033..7049
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7057..7063,7064..7081,7082..7087))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7102..7123
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7240..7257)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7411..7999)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7491..7510)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8170..8961,8962..9030))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8793..8812
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9031..9135)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9210..9229)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
       61 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
      121 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
      181 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      241 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      301 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      361 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      421 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      481 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      541 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      601 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      661 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac
      721 tggcttatcg aaattaatac gactcactat agggagaccc aagcttggta ccgagctcgg
      781 atccactagt aacggccgcc agtgtgctgg aattcgccac catggagcag aagctgatca
      841 gcgaggagga cctgcccagc accagcttcc ccgtgcccag caagttcccc ctgggccccg
      901 ccgccgccgt gttcggcagg ggcgagaccc tgggccccgc ccccagggcc ggcggcacca
      961 tgaagagcgc cgaggaggag cactacggct acgccagcag caacgtgagc cccgccctgc
     1021 ccctgcccac cgcccacagc accctgcccg ccccctgcca caacctccag accagcaccc
     1081 ccggcatcat cccccccgcc gaccacccca gcggctacgg cgccgccctg gacggcggcc
     1141 ccgccggcta cttcctgagc agcggccaca ccaggcccga cggcgccccc gccctggaga
     1201 gccccaggat cgagatcacc agctgcctgg gcctgtacca caacaacaac cagttcttcc
     1261 acgacgtgga ggtggaggac gtgctgccca gcagcaagag gagccccagc accgccaccc
     1321 tgagcctgcc cagcctggag gcctacaggg accccagctg cctgagcccc gccagcagcc
     1381 tgagcagcag gagctgcaac agcgaggcca gcagctacga gagcaactac agctacccct
     1441 acgccagccc ccagaccagc ccctggcaga gcccctgcgt gagccccaag accaccgacc
     1501 ccgaggaggg cttccccagg ggcctgggcg cctgcaccct gctgggcagc cccaggcaca
     1561 gccccagcac cagccccagg gccagcgtga ccgaggagag ctggctgggc gccaggagca
     1621 gcaggcccgc cagcccctgc aacaagagga agtacagcct gaacggcagg cagcccccct
     1681 acagccccca ccacagcccc acccccagcc cccacggcag ccccagggtg agcgtgaccg
     1741 acgacagctg gctgggcaac accacccagt acaccagcag cgccatcgtg gccgccatca
     1801 acgccctgac caccgacagc agcctggacc tgggcgacgg cgtgcccgtg aagagcagga
     1861 agaccaccct ggagcagccc cccagcgtgg ccctgaaggt ggagcccgtg ggcgaggacc
     1921 tgggcagccc cccccccccc gccgacttcg cccccgagga ctacagcagc ttccagcaca
     1981 tcaggaaggg cggcttctgc gaccagtacc tggccgtgcc ccagcacccc taccagtggg
     2041 ccaagcccaa gcccctgagc cccaccagct acatgagcgg cggctctggc ggcggctccg
     2101 gaggctctgt ggacttgagg acactcggtt attcgcaaca gcaacaggag aaaatcaagc
     2161 ctaaggtcag gagcaccgtc gcgcaacacc acgaggcgct tgtggggcat ggcttcactc
     2221 atgcgcatat tgtcgcgctt tcacagcacc ctgcggcgct tgggacggtg gctgtcaaat
     2281 accaagatat gattgcggcc ctgcccgaag ccacgcacga ggcaattgta ggggtcggta
     2341 aacagtggtc gggagcgcga gcacttgagg cgctgctgac tgtggcgggt gagcttaggg
     2401 ggcctccgct ccagctcgac accgggcagc tgctgaagat cgcgaagaga gggggagtaa
     2461 cagcggtaga ggcagtgcac gcctggcgca atgcgctcac cggggccccc ttgaacctga
     2521 ccccagacca ggtagtcgca atcgcgtcaa acggaggggg aaagcaagcc ctggaaaccg
     2581 tgcaaaggtt gttgccggtc ctttgtcaag accacggcct tacaccggag caagtcgtgg
     2641 ccattgcaag caatgggggt ggcaaacagg ctcttgagac ggttcagaga cttctcccag
     2701 ttctctgtca agcccacggg ctgactcccg atcaagttgt agcgattgcg tcgaacattg
     2761 gagggaaaca agcattggag actgtccaac ggctccttcc cgtgttgtgt caagcccacg
     2821 gtttgacgcc tgcacaagtg gtcgccatcg ccagccatga tggcggtaag caggcgctgg
     2881 aaacagtaca gcgcctgctg cctgtactgt gccaggatca tgtactgacc ccagaccagg
     2941 tagtcgcaat cgcgtcaaac ggagggggaa agcaagccct ggaaaccgtg caaaggttgt
     3001 tgccggtcct ttgtcaagac cacggcctta caccggagca agtcgtggcc attgcaaata
     3061 ataacggtgg caaacaggct cttgagacgg ttcagagact tctcccagtt ctctgtcaag
     3121 cccacgggct gactcccgat caagttgtag cgattgcgtc gcatgacgga gggaaacaag
     3181 cattggagac tgtccaacgg ctccttcccg tgttgtgtca agcccacggt ttgacgcctg
     3241 cacaagtggt cgccatcgcc tcgaatggcg gcggtaagca ggcgctggaa acagtacagc
     3301 gcctgctgcc tgtactgtgc caggatcatg gactgacccc agaccaggta gtcgcaatcg
     3361 cgaacaataa tgggggaaag caagccctgg aaaccgtgca aaggttgttg ccggtccttt
     3421 gtcaagacca cggccttaca ccggagcaag tcgtggccat tgcatcccac gacggtggca
     3481 aacaggctct tgagacggtt cagagacttc tcccagttct ctgtcaagcc cacgggctga
     3541 ctcccgatca agttgtagcg attgcgtcca acggtggagg gaaacaagca ttggagactg
     3601 tccaacggct ccttcccgtg ttgtgtcaag cccacggttt gacgcctgca caagtggtcg
     3661 ccatcgccag ccatgatggc ggtaagcagg cgctggaaac agtacagcgc ctgctgcctg
     3721 tactgtgcca ggatcatgga ctgaccccag accaggtagt cgcaatcgcg tcacatgacg
     3781 ggggaaagca agccctggaa accgtgcaaa ggttgttgcc ggtcctttgt caagaccacg
     3841 gccttacacc ggagcaagtc gtggccattg catcccacga cggtggcaaa caggctcttg
     3901 agacggttca gagacttctc ccagttctct gtcaagccca cgggctgact cccgatcaag
     3961 ttgtagcgat tgcgaataac aatggaggga aacaagcatt ggagactgtc caacggctcc
     4021 ttcccgtgtt gtgtcaagcc cacggtttga cgcctgcaca agtggtcgcc atcgccagcc
     4081 atgatggcgg taagcaggcg ctggaaacag tacagcgcct gctgcctgta ctgtgccagg
     4141 atcatggact gacacccgaa caggtggtcg ccattgcttc taatggggga ggacggccag
     4201 ccttggagtc catcgtagcc caattgtcca ggcccgatcc cgcgttggct gcgttaacga
     4261 atgaccatct ggtggcgttg gcatgtcttg gtggacgacc cgcgctcgat gcagtcaaaa
     4321 agggtctgcc tcatgctccc gcattgatca aaagaaccaa ccggcggatt cccgagagaa
     4381 cttcccatcg agtcgcggga tccggcggct ctggcggcgg ctccggaggc tctgaggcca
     4441 gcggttcggg acgggctgac gcattggacg attttgatct ggatatgctg ggaagtgacg
     4501 ccctcgatga ttttgacctt gacatgcttg gttcggatgc ccttgatgac tttgacctcg
     4561 acatgctcgg cagtgacgcc cttgatgatt tcgacctgga catgctgatt aacactagtg
     4621 gcggctctgg cggcggctcc ggaggctctg gcaagaagtt ctggaagagg ctgaggaagt
     4681 tcctgaggaa gctgaagagc taatctagag ggccctattc tatagtgtca cctaaatgct
     4741 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     4801 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     4861 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     4921 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     4981 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     5041 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5101 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5161 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5221 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5281 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5341 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5401 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5461 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     5521 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     5581 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     5641 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     5701 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     5761 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     5821 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     5881 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     5941 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     6001 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     6061 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     6121 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     6181 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     6241 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     6301 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     6361 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     6421 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     6481 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     6541 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     6601 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     6661 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     6721 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     6781 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     6841 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6901 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6961 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     7021 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7081 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7141 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7201 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7261 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7321 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7381 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7441 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7501 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7561 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7621 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7681 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7741 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7801 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7861 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7921 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7981 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8041 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8101 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8161 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8221 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8281 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8341 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8401 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8461 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     8521 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8581 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8641 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8701 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8761 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8821 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8881 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8941 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9001 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9061 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9121 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     9181 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     9241 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaa
//
