LOCUS       sequence-338914-       15357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian fluorescent (EGFP-PEST) reporter plasmid for 
            PDGFRA-D842V+R914W mutant signaling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15357)
  AUTHORS   Yu Y, Alvarado R, Petty LE, Bohlender RJ, Shaw DM, Below JE, Bejar 
            N, Ruiz OE, Tandon B, Eisenhoffer GT, Kiss DL, Huff CD, Letra A, 
            Hecht JT
  TITLE     Polygenic risk impacts PDGFRA mutation penetrance in non-syndromic 
            cleft lip and palate.
  JOURNAL   Hum Mol Genet. 2022 Jul 21;31(14):2348-2357. doi: 
            10.1093/hmg/ddac037.
  PUBMED    35147171
REFERENCE   2  (bases 1 to 15357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Hum Mol Genet"; date: "2022-07-21- 21"; volume: "31"; issue: "14"; 
            pages: "2348-2357"
COMMENT     Sequence Label: pCEP4-myc-PDGFRA-D842V+R914W_EGFP-PEST_reporter
FEATURES             Location/Qualifiers
     source          1..15357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             687..716
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    3964..4098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4241..4263)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4853..6642
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(9161..9179)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9247..9351
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9352..9420,9421..10212)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9570..9589)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10383..10971
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    11733..11781
                     /note="synthetic polyadenylation signal"
     misc_feature    11795..11886
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     11835..11854
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        12065..12096
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             join(12137..12139,12140..12142,12143..12853)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(12137..12139,12140..12142,12143..12853)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(12182..12203)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(12443..12462)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12790..12811
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    13040..13161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        13389..13534
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             13577..14614
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(14612..14631)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    14656..14704
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgccaccat gaagaccatc atcgccctga
      661 gctacatctt ctgcctggtg ttcgccgagc agaaattgat cagcgaggag gacctgggca
      721 gccctggagg cgcctctggc cagctttcat taccctctat ccttccaaat gaaaatgaaa
      781 aggttgtgca gctgaattca tccttttctc tgagatgctt tggggagagt gaagtgagct
      841 ggcagtaccc catgtctgaa gaagagagct ccgatgtgga aatcagaaat gaagaaaaca
      901 acagcggcct ttttgtgacg gtcttggaag tgagcagtgc ctcggcggcc cacacagggt
      961 tgtacacttg ctattacaac cacactcaga cagaagagaa tgagcttgaa ggcaggcaca
     1021 tttacatcta tgtgccagac ccagatgtag cctttgtacc tctaggaatg acggattatt
     1081 tagtcatcgt ggaggatgat gattctgcca ttataccttg tcgcacaact gatcccgaga
     1141 ctcctgtaac cttacacaac agtgaggggg tggtacctgc ctcctatgac agcagacagg
     1201 gctttaatgg gaccttcact gtagggccct atatctgtga ggccaccgtc aaaggaaaga
     1261 agttccagac catcccattt aatgtttatg ctttaaaagc aacatcagag ctggatctag
     1321 aaatggaagc tcttaaaacc gtgtataagt caggggaaac gattgtggtc acctgtgctg
     1381 tttttaacaa tgaggtggtt gaccttcaat ggacttaccc tggagaagtg aaaggcaaag
     1441 gcatcacaat gctggaagaa atcaaagtcc catccatcaa attggtgtac actttgacgg
     1501 tccccgaggc cacggtgaaa gacagtggag attacgaatg tgctgcccgc caggctacca
     1561 gggaggtcaa agaaatgaag aaagtcacta tttctgtcca tgagaaaggt ttcattgaaa
     1621 tcaaacccac cttcagccag ttggaagctg tcaacctgca tgaagtcaaa cattttgttg
     1681 tagaggtgcg ggcctaccca cctcccagga tatcctggct gaaaaacaat ctgactctga
     1741 ttgaaaatct cactgagatc accactgatg tggaaaagat tcaggaaata aggtatcgaa
     1801 gcaaattaaa gctgatccgt gctaaggaag aagacagtgg ccattatact attgtagctc
     1861 aaaatgaaga tgctgtgaag agctatactt ttgaactgtt aactcaagtt ccttcatcca
     1921 ttctggactt ggtcgatgat caccatggct caactggggg acagaccgta cggtgcacag
     1981 ctgaaggcac gccgcttcct gatattgagt ggatgatatg caaagatatt aagaaatgta
     2041 ataatgaaac ttcctggact attttggcca acaatgtctc aaacatcatc acggagatcc
     2101 actcccgaga caggagtacc gtggagggcc gtgtgacttt cgccaaagtg gaggagacca
     2161 tcgccgtgcg atgcctggct aagaatctcc ttggagctga gaaccgagag ctgaagctgg
     2221 tggctcccac cctgcgttct gaactcacgg tggctgctgc agtcctggtg ctgttggtga
     2281 ttgtgatcat ctcacttatt gtcctggttg tcatttggaa acagaaaccg aggtatgaaa
     2341 ttcgctggag ggtcattgaa tcaatcagcc cggatggaca tgaatatatt tatgtggacc
     2401 cgatgcagct gccttatgac tcaagatggg agtttccaag agatggacta gtgcttggtc
     2461 gggtcttggg gtctggagcg tttgggaagg tggttgaagg aacagcctat ggattaagcc
     2521 ggtcccaacc tgtcatgaaa gttgcagtga agatgctaaa acccacggcc agatccagtg
     2581 aaaaacaagc tctcatgtct gaactgaaga taatgactca cctggggcca catttgaaca
     2641 ttgtaaactt gctgggagcc tgcaccaagt caggccccat ttacatcatc acagagtatt
     2701 gcttctatgg agatttggtc aactatttgc ataagaatag ggatagcttc ctgagccacc
     2761 acccagagaa gccaaagaaa gagctggata tctttggatt gaaccctgct gatgaaagca
     2821 cacggagcta tgttatttta tcttttgaaa acaatggtga ctacatggac atgaagcagg
     2881 ctgatactac acagtatgtc cccatgctag aaaggaaaga ggtttctaaa tattccgaca
     2941 tccagagatc actctatgat cgtccagcct catataagaa gaaatctatg ttagactcag
     3001 aagtcaaaaa cctcctttca gatgataact cagaaggcct tactttattg gatttgttga
     3061 gcttcaccta tcaagttgcc cgaggaatgg agtttttggc ttcaaaaaat tgtgtccacc
     3121 gtgatctggc tgctcgcaac gtcctcctgg cacaaggaaa aattgtgaag atctgtgact
     3181 ttggcctggc cagagtcatc atgcatgatt cgaactatgt gtcgaaaggc agtacctttc
     3241 tgcccgtgaa gtggatggct cctgagagca tctttgacaa cctctacacc acactgagtg
     3301 atgtctggtc ttatggcatt ctgctctggg agatcttttc ccttggtggc accccttacc
     3361 ccggcatgat ggtggattct actttctaca ataagatcaa gagtgggtac tggatggcca
     3421 agcctgacca cgctaccagt gaagtctacg agatcatggt gaaatgctgg aacagtgagc
     3481 cggagaagag accctccttt taccacctga gtgagattgt ggagaatctg ctgcctggac
     3541 aatataaaaa gagttatgaa aaaattcacc tggacttcct gaagagtgac catcctgctg
     3601 tggcacgcat gcgtgtggac tcagacaatg catacattgg tgtcacctac aaaaacgagg
     3661 aagacaagct gaaggactgg gagggtggtc tggatgagca gagactgagc gctgacagtg
     3721 gctacatcat tcctctgcct gacattgacc ctgtccctga ggaggaggac ctgggcaaga
     3781 ggaacagaca cagctcgcag acctctgaag agagtgccat tgagacgggt tccagcagtt
     3841 ccaccttcat caagagagag gacgagacca ttgaagacat cgacatgatg gacgacatcg
     3901 gcatagactc ttcagacctg gtggaagaca gcttcctgta atgactcgag gccggcaagg
     3961 ccggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     4021 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     4081 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     4141 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     4201 gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     4261 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     4321 cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgactcta gaggatcgat
     4381 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcgggactac
     4441 gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa
     4501 cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc
     4561 tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt
     4621 tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa
     4681 ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa
     4741 gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga
     4801 ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta
     4861 gcatatgctt cccgggtagt agtatatact atccagacta accctaattc aatagcatat
     4921 gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca
     4981 gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg
     5041 agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga
     5101 actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc
     5161 tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc
     5221 ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac
     5281 cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt
     5341 aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga
     5401 atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg
     5461 tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg
     5521 gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa
     5581 ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt
     5641 tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg
     5701 taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca
     5761 cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc
     5821 caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc
     5881 gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa
     5941 tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     6001 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     6061 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
     6121 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
     6181 tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta
     6241 tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc
     6301 tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     6361 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     6421 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     6481 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct catgcatata
     6541 cagtcagcat atgataccca gtagtagagt gggagtgcta tcctttgcat atgccgccac
     6601 ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc tgctggttgc tcccattctt
     6661 aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc tgattgctca ccaggtaaat
     6721 gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg agctgagtga cgtgacaaca
     6781 tgggtatgcc caattgcccc atgttgggag gacgaaaatg gtgacaagac agatggccag
     6841 aaatacacca acagcacgca tgatgtctac tggggattta ttctttagtg cgggggaata
     6901 cacggctttt aatacgattg agggcgtctc ctaacaagtt acatcactcc tgcccttcct
     6961 caccctcatc tccatcacct ccttcatctc cgtcatctcc gtcatcaccc tccgcggcag
     7021 ccccttccac cataggtgga aaccagggag gcaaatctac tccatcgtca aagctgcaca
     7081 cagtcaccct gatattgcag gtaggagcgg gctttgtcat aacaaggtcc ttaatcgcat
     7141 ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac catgaaataa cagacaatgg
     7201 actcccttag cgggccaggt tgtgggccgg gtccaggggc cattccaaag gggagacgac
     7261 tcaatggtgt aagacgacat tgtggaatag caagggcagt tcctcgcctt aggttgtaaa
     7321 gggaggtctt actacctcca tatacgaaca caccggcgac ccaagttcct tcgtcggtag
     7381 tcctttctac gtgactccta gccaggagag ctcttaaacc ttctgcaatg ttctcaaatt
     7441 tcgggttgga acctccttga ccacgatgct ttccaaacca ccctcctttt ttgcgcctgc
     7501 ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc tcctgggtca tctgcggggc
     7561 cctgctctat cgctcccggg ggcacgtcag gctcaccatc tgggccacct tcttggtggt
     7621 attcaaaata atcggcttcc cctacagggt ggaaaaatgg ccttctacct ggagggggcc
     7681 tgcgcggtgg agacccggat gatgatgact gactactggg actcctgggc ctcttttctc
     7741 cacgtccacg acctctcccc ctggctcttt cacgacttcc ccccctggct ctttcacgtc
     7801 ctctaccccg gcggcctcca ctacctcctc gaccccggcc tccactacct cctcgacccc
     7861 ggcctccact gcctcctcga ccccggcctc cacctcctgc tcctgcccct cctgctcctg
     7921 cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg
     7981 ctcctgctcc tgttcccccg tgggtccctt ttccgccact gccacctggc cgttttttgg
     8041 gtctcccgcc cccctctcct cttccttttc ccgttcctcc tgcycctscc cctcctbccc
     8101 ctcctgctcc cgcccctcct gccccycctg ctccctcctg cccctcctgc tcctgcccct
     8161 cctgcccctc ctgctcctgc tcctgttcta cacccgcgcc bctcaccccm cccccccccc
     8221 cccccccccc sccccctccc ccccctcccc cccccccccc tccccccccc ccccccccgc
     8281 ccccctcccc tacctcctcg accccggcct ccactacctc ctcgaccccg gcctccactg
     8341 cctcctcgac cccggcctcc acctcctgct cctgcccctc ctgctcctgc ccctcctcct
     8401 gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgctcct
     8461 gttccaccgt gggtcccttt gcagccaatg caacttggac gtttttgggg tctccggaca
     8521 ccatctctat gtcttggccc tgatcctgag ccgcccgggg ctcctggtct tccgcctcct
     8581 cgtcctcgtc ctcttccccg tcctcgtcca tggttatcac cccctcttct ttgaggtcca
     8641 ctgccgccgg agccttctgg tccagatgtg tctcccttct ctcctaggcc atttccaggt
     8701 cctgtacctg gcccctcgtc agacatgatt cacactaaaa gagatcaata gacatcttta
     8761 ttagacgacg ctcagtgaat acagggagtg cagactcctg ccccctccaa cagccccccc
     8821 accctcatcc ccttcatggt cgctgtcaga cagatccagg tctgaaaatt ccccatcctc
     8881 cgaaccatcc tcgtcctcat caccaattac tcgcagcccg gaaaactccc gctgaacatc
     8941 ctcaagattt gcgtcctgag cctcaagcca ggcctcaaat tcctcgtccc cctttttgct
     9001 ggacggtagg gatggggatt ctcgggaccc ctcctcttcc tcttcaaggt caccagacag
     9061 agatgctact ggggcaacgg aagaaaagct gggtgcggcc tgtgaggatc agcttatcga
     9121 tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt
     9181 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     9241 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     9301 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     9361 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     9421 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     9481 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     9541 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     9601 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     9661 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     9721 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     9781 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     9841 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     9901 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     9961 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
    10021 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
    10081 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
    10141 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    10201 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    10261 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    10321 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    10381 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    10441 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    10501 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
    10561 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10621 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10681 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    10741 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    10801 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    10861 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    10921 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    10981 aacgcggcct ttttacggtt cctggccttt tgctggcctt gaagctgtcc ctgatggtcg
    11041 tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc tccgcaagaa
    11101 ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ctgcttcatc
    11161 cccgtggccc gttgctcgcg tttgctgtcc agggggaaac gcctggtatc tttatagtcc
    11221 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    11281 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    11341 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
    11401 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
    11461 cgcaaggatc ttgccgctat tgagatccag ttcgatatag cccactcttg cacccagttg
    11521 atcttcagca tcttttactt tcaccagcgt ttcggggtgt gcaaaaacag gcaagcaaaa
    11581 tgccgcaaag aagggaatga gtgcgacacg aaaatgttgg atgctcatac tcgtcctttt
    11641 tcaatattat tgaagcattt atcagggtta ctagtacgtc tctcaaggat aagtaagtaa
    11701 tattaaggta cgggaggtat tggacaggcc gcaataaaat atctttattt tcattacatc
    11761 tgtgtgttgg ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa
    11821 cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat
    11881 ttctctggcc taactggccg gtacctgagc tcactagcag gatgtccata ttaggacatc
    11941 taggatgtcc atattaggac atctaggatg tccatattag gacatctagg atgtccatat
    12001 taggacatct aggatgtcca tattaggaca tctagatctg gcctcggcgg ccaagcttag
    12061 acactagagg gtatataatg gaagctcgac ttccagcttg gcaatccggt actgttggta
    12121 aagccaccgg cgcgccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
    12181 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
    12241 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
    12301 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
    12361 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
    12421 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
    12481 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
    12541 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
    12601 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
    12661 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
    12721 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
    12781 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
    12841 cgagctgtac aagagtcatg gttttccccc ggaagtcgaa gagcaggacg acgggacgct
    12901 gccaatgtca tgcgcacaag agtctggtat ggatcgccac ccggctgcgt gtgccagcgc
    12961 gagaatcaat gtataatgag gcgcgcccta aggccgcgac tctagagtcg gggcggccgg
    13021 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
    13081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
    13141 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
    13201 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
    13261 aagcgaacgc cagcaagacg tagcccagcg ytagcgaggt gccgccctgc ttcatccccg
    13321 tggcccgttg ctcgcgtttg ctggcggtgt ccccggaaga aatatatttg catgtcttta
    13381 gttctatgat gacacaaacc ccgcccagcg tcttgtcatt ggcgaattcg aacacgcaga
    13441 tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat taaggtgacg cgtgtggcct
    13501 cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt caacagcgtg ccgcagatcc
    13561 cggggggcaa tgagatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct
    13621 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
    13681 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
    13741 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
    13801 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
    13861 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
    13921 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
    13981 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
    14041 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
    14101 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
    14161 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
    14221 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
    14281 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
    14341 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
    14401 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
    14461 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
    14521 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
    14581 tcgtccggat cgggagatgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
    14641 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt
    14701 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
    14761 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
    14821 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc cactggcccc
    14881 gtgggttagg gacggggtcc cccatgggga atggtttatg gttcgtgggg gttattattt
    14941 tgggcgttgc gtggggtcag gtccacgact ggactgagca gacagaccca tggtttttgg
    15001 atggcctggg catggaccgc atgtactggc gcgacacgaa caccgggcgt ctgtggctgc
    15061 caaacacccc cgacccccaa aaaccaccgc gcggatttct ggcgtgccaa gctagtcgac
    15121 caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc
    15181 aggcgcagaa ctggtaggta tggaagatct atacattgaa tcaatattgg caattagcca
    15241 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
    15301 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgtt
//
