LOCUS       sequence-338927-        2811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA donor for transposition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Zhang Y, Yang J, Yang S, Zhang J, Chen J, Tao R, Jiang Y, Yang J, 
            Yang S
  TITLE     Programming Cells by Multicopy Chromosomal Integration Using 
            CRISPR-Associated Transposases.
  JOURNAL   CRISPR J. 2021 Jun;4(3):350-359. doi: 10.1089/crispr.2021.0018.
  PUBMED    34152213
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2021"; journalName: "CRISPR J"; date: "2021-06"; 
            volume: "4"; issue: "3"; pages: "350-359"
COMMENT     Sequence Label: pRE57-Ter
FEATURES             Location/Qualifiers
     source          1..2811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             176..198
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      224..255
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     terminator      368..416
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     terminator      478..526
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     primer_bind     complement(595..617)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      complement(1056..1644)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1136..1155)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1815..2606,2607..2675))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2438..2457
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2676..2780)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccaatgatgc gaaaaaaccc cgccgaagcg gggttttttg cgaaaaaaat agggataaca
       61 gggtaatgtt taaactgttg atacaaccat aaaatgataa ttacacccat aaattgataa
      121 ttatcacacc cacgggaatt ccatggtcga catgcataag cttcctagga ataattttgt
      181 ttaactttaa gaaggagata tacatatggc tagctaagca gaaagtcaaa agcctccgac
      241 cggaggcttt tgactattgc tcaacaggta aggcgcgagg ttacgcgtct cgagttaatt
      301 aaactagtga gctcggtacc cggggatcct ctagaggtcg aaattcacct cgaaagcaag
      361 ctgataaacc gatacaatta aaggctcctt ttggagcctt tttttttgga gattttcaac
      421 gtgaaaaaat tattattcgc aattccaagc taattcacct cgaaagcaag ctgataaacc
      481 gatacaatta aaggctcctt ttggagcctt tttttttgga gattttcaac gtgaaaaaat
      541 tattattcgc aattccaagc tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
      601 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
      661 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
      721 acgtagcgat agcggagtgt atgggctcga tcccctcgga tccaattggt ccttactgca
      781 gtagttttgc tgaaatactc gattcacaaa aatatcaact tatggttgtt ttgtgagata
      841 tcaatatatg gttgttttgt ggttaagttg ctgattataa ataattatta aatatcactt
      901 tatggttgca tcaacagtta acattaccct gttatcccta aaaaaaaaac cccgcccctg
      961 acagggcggg gttttttttt taatatcgga aagaacatgt gagcaaaagg ccagcaaaag
     1021 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     1081 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1141 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1201 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1261 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1321 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1381 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1441 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     1501 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1561 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1621 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1681 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1741 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1801 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1861 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1921 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1981 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     2041 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     2101 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     2161 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     2221 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     2281 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     2341 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     2401 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     2461 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     2521 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     2581 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     2641 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     2701 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2761 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
