LOCUS       sequence-33923-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  TITLE     Victoria Prince lab plasmids
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBS ZfHoxb5a
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..26
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10..26
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(647..663)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(648..664)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(688..707)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(689..707)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(717..734)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(717..733)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(726..748)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ggcggccgct ctagaactag tggatccgac caagatctac aaatcaagcc ctctaaaaca
       61 acctaaatga gctcttactt tgtaaactcg ttctcagggc gctacccaaa tggccctgac
      121 tatcagttac taaattatgg aactagcagc agcgctatga acgcttcgta cagggactcc
      181 ggcaccatgc attcaggctc ttacggctac aactacaatg gaatggacct aagcgtcaat
      241 cgttcaacca gcactggcca ctttggggcg gttggggaca actcccgggt cttccagtct
      301 ccagctccgg agacgcgatt ccggcagcca tcaagctgct cgctcgcgtc tccggagccg
      361 ctgccttgtt ccaacagcga aagccttgga cccaaaggct cttcgccccc ttccgaccaa
      421 agcaccacca ctgcgggtaa taatctcaac agcaacacac attttacaga aatcgacgag
      481 gccagtgctt catcggagac cgaggaggcg tctcacagag cgaacaactc tgcaccccgg
      541 acacaacaga agcaagagac cacggcaacc tccactacct cggcgacgtc cgatggccaa
      601 gctccccaaa tattcccttg gatgaggaat tcgatatcaa gcttatcgat accgtcgacc
      661 tcgagggggg gcccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
      721 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      781 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      841 tcccaacagt tgcgcagcct gaatggcgaa tggaaattgt aagcgttaat attttgttaa
      901 aattcgcgtt aaatttttgt t
//
