LOCUS       sequence-339301-        2607 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning plasmid for creating BcLOV4 optogenetic tool fusions. 
            Expresses 
            [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a 
            pcDNA3.1 backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Berlew EE, Yamada K, Kuznetsov IA, Rand EA, Ochs CC, Jaber Z, 
            Gardner KH, Chow BY
  TITLE     Designing Single-Component Optogenetic Membrane Recruitment Systems:
            The Rho-Family GTPase Signaling Toolbox.
  JOURNAL   ACS Synth Biol. 2022 Jan 21;11(1):515-521. doi: 
            10.1021/acssynbio.1c00604. Epub 2022 Jan 3.
  PUBMED    34978789
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00604"; journalName: "ACS Synth Biol"; date: 
            "2022-01-21- 21"; volume: "11"; issue: "1"; pages: "515-521"
COMMENT     Sequence Label: BcLOV4 fusion tools screening plasmid #2
FEATURES             Location/Qualifiers
     source          1..2607
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..708
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(146..164)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     414..433
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2539..2604
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
       61 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      121 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      181 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      241 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
      301 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      361 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
      421 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
      481 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
      541 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
      601 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
      661 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagct cgagggtggt
      721 ggatcaggtg gaggatctat ggccacagac gcaatcgacc attactacca gaacagagac
      781 ggcccccctt ccaggagctt tagcgacgaa tacgactcat acaataagac agcccctctg
      841 ccacctctga gcgcctgccc agagcgcgcc cagaccccta acagcaagag aatccccatc
      901 acacccaatt ccgccaagag cacctccgag tctcctcgga gaatcacatt ctctcgcgac
      961 gcaggcagct ccaccaaggg cagcacccag acaagcccat ccaaccaggg ctccgtgtct
     1021 agccggaatg gctctaacgg aaatggcctg ggcctgtcct ctgccggcaa cttcgccgat
     1081 ttctttagct ccgaggtgtt ttatatcgtg ctgcacaatc ccaccacagc ccacagattc
     1141 ctgaagtttt gccagagcag ggcctgttcc gagaacctgg agtttctgca gaagatcgac
     1201 gagtacaata ggctgatcga tgaggccaca cgcctgctgt ctaccatcta cagcacatac
     1261 gtgagcagcg aggcccctaa gcagatcaac atctctaata gccacgccag gcgcctgagc
     1321 cacgacatca agggagcaac ccacaacgtg ctgcccggcc tggaggatat cttcaatggc
     1381 tcccaggagc agatcgagaa gctgctggcc tctgacctgt atcctagatt tgtgaagcac
     1441 caggtgaccg catccgccac aatggccctg gcaaacgaca aggagaggta cccaggcctg
     1501 ggcgattgct tctgtctgac agacccacgg agagccgata atcccatcgt gttcgcaagc
     1561 gacggattcg tgagcgtgac cggctattcc cgctctgata tcatcccaag gaactgccgc
     1621 ttcctgcagg gcagctttac cgaccggcag gccacaaaga ggctgcgcac ctccatcgag
     1681 aatcgcgagg agacagtgga gctgctgctg aactatcgga agaatggcga tcccttttgg
     1741 aacctgctgt acgtgtcccc tctgctggac ggcaatggcg atgtgagatt ctttctgggc
     1801 ggccagatca actgttctac cacaatccac agccggaccg acgtgctgag aatcctgagc
     1861 ctgaacgacg aggatacagg ctccttcatc gagggctcta ataagacccc cagcgtgcgg
     1921 tccaaggagt ctaacaatgc ctccaactat ggcaagacat ccttctttaa gtcttttaag
     1981 aagtacaagg ccaataccgt ggagatcaga gacgaggccg gcatggaggg cgagctgatc
     2041 gataggatcg gcaagctgca gttcaagaca caggtggagg agttttacac cgcctattct
     2101 aagtacctgg tgctgagcta tcagccacag tcccagaacc tgatcgtgaa gtacttcagc
     2161 cccggcatcg tggatatgct gaacctgaat atgcctaatg gcctggtggc cccaatcgtg
     2221 gacaaggata tcttcaaggt gctgacagag cactcccctt cctctgtgcc acggaccttt
     2281 aagaacgtgg tgagggaagg catcaaagca ggcagggccg tgtctgtgga gacaggcctg
     2341 ctgacaggca tcgaggagat caagaagtct cagaacatct tcagcggcac cacagccaat
     2401 cgggagagag gctggaagaa ggccgaggag aagtacgtgt gccactttac cccctgtaag
     2461 gatgagatgg gcagggtcgg ctgggtggtg ctgactgtcg ctcctaaact ggaaagattt
     2521 gaattcggtg gtggatctga ctacaaagat catgacgggg attataagga ccacgacatc
     2581 gattacaagg atgacgatga caagtaa
//
