LOCUS       sequence-339484-        3580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression plasmid for recombinant His-Myc tag human PLD4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Gavin AL, Huang D, Blane TR, Thinnes TC, Murakami Y, Fukui R, Miyake
            K, Nemazee D
  TITLE     Cleavage of DNA and RNA by PLD3 and PLD4 limits autoinflammatory 
            triggering by multiple sensors.
  JOURNAL   Nat Commun. 2021 Oct 7;12(1):5874. doi: 10.1038/s41467-021-26150-w.
  PUBMED    34620855
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-10-7"; volume: "12(1):5874. doi"; pages: " 
            10.1038/s41467-021-26150-w"
COMMENT     Sequence Label: SuExp His-Myc-hPLD4
FEATURES             Location/Qualifiers
     source          1..3580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          314..410
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             426..443
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             471..500
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             501..530
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="ASMTGGQQMG"
     CDS             537..548
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    1934..2068
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1958..1977)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2012..2031
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(2111..2699)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2191..2210)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2724..3098)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(3099..3162)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        3201..3580
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgtcga
      241 gccgagtggt tgtgcctcca tagaagccac catgacccgc ctgaccgtgc tggccctgct
      301 ggccggcctg ctggtaagtt tagtcttttt gtcttttatt tcaggtcccg gatccggtgg
      361 tggtgcaaat caaagaactg ctcctcagtg gatgttgcct ttacttctag gccagcagcc
      421 gcgcccatca tcatcatcat catggtccgc tagttgacgt cgccagcaat gaacaaaaac
      481 tcatctcaga agaggatctg gctagtatga ctggtggaca gcaaatgggt cgggatatcg
      541 aaggtcgtgg gctagctcaa gtgccccgtc ctcccacctg gggccaggtg cagcccaagg
      601 acgtgcccag gtcctgggag catggctcca gcccagcttg ggagcccctg gaagcagagg
      661 ccaggcagca gagggactcc tgccagcttg tccttgtgga aagcatcccc caggacctgc
      721 catctgcagc cggcagcccc tctgcccagc ctctgggcca ggcctggctg cagctgctgg
      781 acactgccca ggagagcgtc cacgtggctt catactactg gtccctcaca gggcctgaca
      841 tcggggtcaa cgactcgtct tcccagctgg gagaggctct tctgcagaag ctgcagcagc
      901 tgctgggcag gaacatttcc ctggctgtgg ccaccagcag cccgacactg gccaggacat
      961 ccaccgacct gcaggttctg gctgcccgag gtgcccatgt acgacaggtg cccatggggc
     1021 ggctcaccag gggtgttttg cactccaaat tctgggttgt ggatggacgg cacatataca
     1081 tgggcagtgc caacatggac tggcggtctc tgacgcaggt gaaggagctt ggcgctgtca
     1141 tctataactg cagccacctg gcccaagacc tggagaagac cttccagacc tactgggtac
     1201 tgggggtgcc caaggctgtc ctccccaaaa cctggcctca gaacttctca tctcacttca
     1261 accgtttcca gcccttccac ggcctctttg atggggtgcc caccactgcc tacttctcag
     1321 cgtcgccacc agcactctgt ccccagggcc gcacccggga cctggaggcg ctgctggcgg
     1381 tgatggggag cgcccaggag ttcatctatg cctccgtgat ggagtatttc cccaccacgc
     1441 gcttcagcca ccccccgagg tactggccgg tgctggacaa cgcgctgcgg gcggcagcct
     1501 tcggcaaggg cgtgcgcgtg cgcctgctgg tcggctgcgg actcaacacg gaccccacca
     1561 tgttccccta cctgcggtcc ctgcaggcgc tcagcaaccc cgcggccaac gtctctgtgg
     1621 acgtgaaagt cttcatcgtg ccggtgggga accattccaa catcccattc agcagggtga
     1681 accacagcaa gttcatggtc acggagaagg cagcctacat aggcacctcc aactggtcgg
     1741 aggattactt cagcagcacg gcgggggtgg gcttggtggt cacccagagc cctggcgcgc
     1801 agcccgcggg ggccacggtg caggagcagc tgcggcagct ctttgagcgg gactggagtt
     1861 cgcgctacgc cgtcggcctg gacggacagg ctccgggcca ggactgcgtt tggcagggct
     1921 aaatctagaa gctgatccag acatgataag atacattgat gagtttggac aaaccacaac
     1981 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     2041 aaccattata agctgcaata aacaagttgg atatcctcga gactagtggg cccgtttaaa
     2101 cacatgtgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2161 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2221 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2281 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2341 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2401 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2461 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2521 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     2581 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2641 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2701 aagatccttt gatcttttct acgtcagtcc tgctcctcgg ccacgaagtg cacgcagttg
     2761 ccggccgggt cgcgcagggc gaactcccgc ccccacggct gctcgccgat ctcggtcatg
     2821 gccggcccgg aggcgtcccg gaagttcgtg gacacgacct ccgaccactc ggcgtacagc
     2881 tcgtccaggc cgcgcaccca cacccaggcc agggtgttgt ccggcaccac ctggtcctgg
     2941 accgcgctga tgaacagggt cacgtcgtcc cggaccacac cggcgaagtc gtcctccacg
     3001 aagtcccggg agaacccgag ccggtcggtc cagaactcga ccgctccggc gacgtcgcgc
     3061 gcggtgagca ccggaacggc actggtcaac ttggccatac tcttcctttt tcaatattat
     3121 tgaagcattt atcagggtta ttgtctcatg agcggataca taacgcgtcg cgaggccata
     3181 tgggttaacc catggccgtt gacattgatt attgactagt tattaatagt aatcaattac
     3241 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     3301 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     3361 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     3421 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     3481 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     3541 ttggcagtac atctacgtat tagtcatcgc tattaccatg
//
