LOCUS       sequence-339555-        6330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CCR6 receiver construct in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Cho KF, Gillespie SM, Kalogriopoulos NA, Quezada MA, Jacko M, Monje 
            M, Ting AY
  TITLE     A light-gated transcriptional recorder for detecting cell-cell 
            contacts.
  JOURNAL   Elife. 2022 Mar 21;11:e70881. doi: 10.7554/eLife.70881.
  PUBMED    35311648
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-03-21"; volume: "11:e70881. doi"; pages: " 
            10.7554/eLife.70881"
COMMENT     Sequence Label: pAAV syn CCR6-eLOV-TEVcs-tTA
FEATURES             Location/Qualifiers
     source          1..6330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        181..628
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             2253..2273
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             2286..2309
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(2310..2930,2931..3317)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(2383..2402)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(3351..3368)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3357..3564
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3593..3733
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3593..3722
                     /label=AAV2 ITR
     rep_origin      3808..4263
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3895..3914)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4105..4126
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4280..4299)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4399..4421
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4459..4477)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4545..4649
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4650..4718,4719..5510)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4868..4887)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5681..6269
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6170..6189
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaca gtgtctagac tgcagagggc cctgcgtatg
      181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      601 aggagtcgtg tcgtgcctga gagcgcagtc gagaaggatc cgccaccatg agcggagaga
      661 gcatgaactt cagtgatgtg ttcgattcca gtgaggacta cttcgtgtcc gtcaatacat
      721 cctactacag cgtagattct gagatgctgc tttgctccct gcaggaggtg cgccagttct
      781 cccgactctt cgttcccatc gcgtattcac tcatctgcgt ctttggcctc ctggggaata
      841 tcttggtagt catcacattt gctttttata aaaaggccag atccatgaca gacgtctacc
      901 tgcttaatat ggccattgca gacatcctgt tcgtcctgac gctcccattc tgggctgtga
      961 gtcacgctac cggggcctgg gtgttcagta atgcaacctg caagctgttg aagggaattt
     1021 atgccatcaa ctttaattgc ggtatgctgc tgctgacttg cataagtatg gacagataca
     1081 tcgccatagt acaggcaaca aaatccttca gactgaggtc tcgaacgctc cctaggtcta
     1141 aaatcatttg tcttgttgta tggggcttga gcgtgattat ttccagcagt actttcgtgt
     1201 ttaaccagaa gtacaacacg cagggcagcg atgtgtgtga gcccaagtac cagacggtat
     1261 ctgagcctat caggtggaag ctgttgatgc tgggtttgga attgctgttc ggctttttta
     1321 tcccactcat gtttatgatt ttttgttata ccttcatcgt gaagaccctg gtccaggcac
     1381 aaaactctaa gcgacacaag gccatccggg taatcatcgc tgttgtgctg gtatttctgg
     1441 cttgccagat ccctcataat atggtccttc tggtaacggc agccaacctg ggcaaaatga
     1501 atcgctcatg tcagtcagaa aagcttatcg ggtacaccaa aacggttacg gaggttctgg
     1561 cgttcctgca ttgttgcctt aaccctgtgc tgtacgcatt tataggtcag aagtttcgaa
     1621 actacttcct gaagattctc aaagacctct ggtgtgtaag aagaaagtat aaaagtagtg
     1681 gattttcatg cgcaggacgc tactccgaaa acatttccag acagaccagt gagacggccg
     1741 ataacgataa cgcctcctcc tttaccatga ctagtgagct ggccgagaag ctggccggcc
     1801 tggacatcaa cggtggagct agcggcagcc gggcgaccac cctggaacgt attgaaaaga
     1861 gctttgtgat taccgatccg cgtctgccgg ataacccgat tatttttgtg agcgatagct
     1921 ttctgcagct gaccgaatat agccgtgaag aaattctggg ccgtaactgc cgttttctgc
     1981 agggcccgga aaccgatcgt gcgaccgtgc gtaaaattcg tgatgcgatt gataaccaga
     2041 ccgaagtgac cgtgcagctg attaactata ccaaaagcgg caaaaaattt tggaacctgt
     2101 ttcatctgca gccgatgcgt gatcagaaag gcgatgtgca gtattttatt ggcgtgcagc
     2161 tggatggtac cgaacgtgtg cgtgatgcgg cggaacgtga agctgtgatg ctggtcaaga
     2221 aaaccgcgga ggaaatcgac gaagccgcta aggaaaatct ctacttccag atgggcggag
     2281 gatccgacta caaggatgac gacgataaga tgtctagatt agataaaagt aaagtgatta
     2341 acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg
     2401 cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt
     2461 tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag
     2521 aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac
     2581 taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg
     2641 aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat
     2701 tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc
     2761 atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac
     2821 gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg
     2881 aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gcgtacagcc
     2941 gcgcgcgtac gaaaaacaat tacgggtcta ccatcgaggg cctgctcgat ctcccggacg
     3001 acgacgcccc cgaagaggcg gggctggcgg ctccgcgcct gtcctttctc cccgcgggac
     3061 acacgcgcag actgtcgacg gcccccccga ccgatgtcag cctgggggac gagctccact
     3121 tagacggcga ggacgtggcg atggcgcatg ccgacgcgct agacgatttc gatctggaca
     3181 tgttggggga cggggattcc ccgggtccgg gatttacccc ccacgactcc gccccctacg
     3241 gcgctctgga tatggccgac ttcgagtttg agcagatgtt taccgatgcc cttggaattg
     3301 acgagtacgg tgggtaatag gaattcctcg agctagagct cgctgatcag cctcgactgt
     3361 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     3421 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     3481 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     3541 agacaatagc aggcatgctg gggaggtaac cacgtgcgga ccgagcggcc gcaggaaccc
     3601 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     3661 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     3721 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     3781 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     3841 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt
     3901 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     3961 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4021 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4081 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact
     4141 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa
     4201 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     4261 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     4321 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     4381 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     4441 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     4501 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4561 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4621 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4681 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4741 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4801 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4861 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4921 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4981 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5041 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5101 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5161 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5221 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5281 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5341 ataaatctgg agccggtgag cgtggaagcc gcggtatcat tgcagcactg gggccagatg
     5401 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5461 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5521 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5581 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5641 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5701 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5761 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5821 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5881 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5941 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6001 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6061 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6121 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6181 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6241 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6301 tggccttttg ctggcctttt gctcacatgt
//
