LOCUS       sequence-339575-        3495 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC25A44_STOP
FEATURES             Location/Qualifiers
     source          1..3495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1052..1151)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1168..1187)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1169..1187)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1192..1208)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1192..1208)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1310..1329)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1321..2130
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1388..1407)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2277..2865
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2766..2785
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3019..3036
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3195..3222)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3314..3400)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3449..3471
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3463..3480
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3464..3480
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga ggacaagagg
      121 aacatccaga tcatcgagtg ggagcacctg gataagaaga agttctacgt gttcggcgtg
      181 gccatgacca tgatgatccg ggtgagcgtg taccctttca ccctgatccg gacaagactg
      241 caggtgcaga agggcaagag cctgtaccac ggcaccttcg acgcctttat caagatcctg
      301 agggccgatg gcatcacagg cctgtatcgc ggcttcctgg tgaacacctt tacactgatc
      361 agcggccagt gctacgtgac cacatatgag ctgacccgga agtttgtggc cgactacagc
      421 cagtccaata cagtgaagtc cctggtggca ggaggatctg ccagcctggt ggcccagtcc
      481 atcaccgtgc caatcgatgt ggtgtctcag cacctgatga tgcagcggaa gggcgagaag
      541 atgggccggt tccaggtgag aggaaaccca gagggacagg gagtggtggc ctttggccag
      601 accaaggaca tcatcaggca gatcctgcag gcagatggac tgaggggctt ctacaggggc
      661 tatgtggcct ctctgctgac atacatccca aatagcgccg tgtggtggcc cttctaccac
      721 ttttatgccg agcagctgtc ttatctgtgc cccaaggagt gtcctcacat cgtgttccag
      781 gccgtgagcg gacctctggc agcagccacc gcctccatcc tgacaaaccc aatggacgtg
      841 atcaggaccc gcgtgcaggt ggagggcaag aattccatca tcctgacatt tcggcagctg
      901 atggcagagg agggaccatg gggactgatg aagggactgt ctgccagaat catctccgcc
      961 accccttcta caatcgtgat cgtggtgggc tatgagagcc tgaagaagct gtccctgcgg
     1021 cccgagctgg tggattccag acactggtaa gacccagctt tcttgtacaa agttggcatt
     1081 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     1141 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     1201 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     1261 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     1321 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
     1381 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     1441 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     1501 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     1561 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     1621 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     1681 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     1741 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     1801 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     1861 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     1921 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     1981 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     2041 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     2101 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     2161 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     2221 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2281 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2341 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2401 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2461 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2521 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2581 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2641 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2701 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2761 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2821 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2881 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2941 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3001 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     3061 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     3121 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
     3181 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     3241 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     3301 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     3361 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     3421 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     3481 cttaagctcg ggccc
//
