LOCUS       sequence-33964-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Egan DF, Shackelford DB, Mihaylova MM, Gelino S, Kohnz RA, Mair W, 
            Vasquez DS, Joshi A, Gwinn DM, Taylor R, Asara JM, Fitzpatrick J, 
            Dillin A, Viollet B, Kundu M, Hansen M, Shaw RJ
  TITLE     Phosphorylation of ULK1 (hATG1) by AMP-activated protein kinase 
            connects energy sensing to mitophagy.
  JOURNAL   Science. 2011 Jan 28;331(6016):456-61. doi: 10.1126/science.1196371.
            Epub 2010 Dec 23.
  PUBMED    21205641
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1196371"; journalName: "Science"; date: 
            "2011-01-28- 28"; volume: "331"; issue: "6016"; pages: "456-61"
COMMENT     Sequence Label: pLKO human ULK2 shRNA 91
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    128..245
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        294..804
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     723..741
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 ctttatatat cttgtggaaa ggacgaaaca ccgggtcagt ggtattcgca tcaaactcga
       61 gtttgatgcg aataccactg actttttaat tctcgacctc gagacaaatg gcagtattca
      121 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
      181 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
      241 attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc gagggggttg
      301 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
      361 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
      421 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
      481 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
      541 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
      601 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
      661 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
      721 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
      781 cgaatcaccg acctctctcc ccagggggat ccaccggagc ttaccatgac cgagtacagc
//
