LOCUS       sequence-33967-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Egan DF, Shackelford DB, Mihaylova MM, Gelino S, Kohnz RA, Mair W, 
            Vasquez DS, Joshi A, Gwinn DM, Taylor R, Asara JM, Fitzpatrick J, 
            Dillin A, Viollet B, Kundu M, Hansen M, Shaw RJ
  TITLE     Phosphorylation of ULK1 (hATG1) by AMP-activated protein kinase 
            connects energy sensing to mitophagy.
  JOURNAL   Science. 2011 Jan 28;331(6016):456-61. doi: 10.1126/science.1196371.
            Epub 2010 Dec 23.
  PUBMED    21205641
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1196371"; journalName: "Science"; date: 
            "2011-01-28- 28"; volume: "331"; issue: "6016"; pages: "456-61"
COMMENT     Sequence Label: pcdna3 flag - ULK2 wt
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      81..90
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             90..113
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct gccaccatgg actacaagga cgacgacgat aaggaattcg
      121 ttatcacaag tttgtacaaa aaagcaggct tcgaggtggt gggcgacttc gagtactgca
      181 agcgggacct cgtgggacac ggggccttcg ctgtggtctt ccgggggcgg caccgccaga
      241 aaactgattg ggaggtggct attaaaagta ttaataaaaa gaacttgtca aaatcacaaa
      301 ttctgcttgg aaaggaaata aaaatcttaa aggagcttca gcatgaaaac atcgtagcgc
      361 tctatgatgt tcaggaattg cccaactctg tctttctggt gatggagtat tgcaatggtg
      421 gagacctggc agattatttg caagctaaag gaactctgag tgaagatact atcagagtgt
      481 ttctccatca gattgcggca gccatgcgaa tcctgcacag caaagggata atccacaggg
      541 atctcaaacc acagaatatc ctgttgtctt atgccaatcg aaggaagtcg aatgtcagtg
      601 gtattcgtat taaaatagct gattttggtt tcgcacggta cctacatagt aacacaatgg
      661 cagcgacact gtgtggatcc ccaatgtaca tggctcccga ggttattatg tctcaacatt
      721 atgatgctaa ggcagattta tggagcatag gaacagtgat ctatcaatgc ctagttggaa
      781 aaccaccttt tcaggctaat agtcctca
//
