LOCUS       sequence-339763-        2688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression and purification, combined with SAE1 to form 
            the E1 enzyme heterodimer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Peeples W, Rosen MK
  TITLE     Mechanistic dissection of increased enzymatic rate in a 
            phase-separated compartment.
  JOURNAL   Nat Chem Biol. 2021 Jun;17(6):693-702. doi: 
            10.1038/s41589-021-00801-x. Epub 2021 May 25.
  PUBMED    34035521
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00801-x"; journalName: "Nat Chem Biol"; date: 
            "2021-06"; volume: "17"; issue: "6"; pages: "693-702"
COMMENT     Sequence Label: SAE2-mGarnet
FEATURES             Location/Qualifiers
     source          1..2688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggcgaattct ctggcggctc tggcggctcc ggtggttcca tggcactgtc gcgggggctg
       61 ccccgggagc tggctgaggc ggtggccggg ggccgggtgc tggtggtggg ggcgggcggc
      121 atcggctgcg agctcctcaa gaatctcgtg ctcaccggtt tctcccacat cgacctgatt
      181 gatctggata ctattgatgt aagcaacctc aacagacagt ttttgtttca aaagaaacat
      241 gttggaagat caaaggcaca ggttgccaag gaaagtgtac tgcagtttta cccgaaagct
      301 aatatcgttg cctaccatga cagcatcatg aaccctgact ataatgtgga atttttccga
      361 cagtttatac tggttatgaa tgctttagat aacagagctg cccgaaacca tgttaataga
      421 atgtgcctgg cagctgatgt tcctcttatt gaaagtggaa cagctgggta tcttggacaa
      481 gtaactacta tcaaaaaggg tgtgaccgag tgttatgagt gtcatcctaa gccgacccag
      541 agaacctttc ctggctgtac aattcgtaac acaccttcag aacctataca ttgcatcgtt
      601 tgggcaaagt acttgttcaa ccagttgttt ggggaagaag atgctgatca agaagtatct
      661 cctgacagag ctgaccctga agctgcctgg gaaccaacgg aagccgaagc cagagctaga
      721 gcatctaatg aagatggtga cattaaacgt atttctacta aggaatgggc taaatcaact
      781 ggatatgatc cagttaaact ttttaccaag ctttttaaag atgacatcag gtatctgttg
      841 acaatggaca aactatggcg gaaaaggaaa cctccagttc cgttggactg ggctgaagta
      901 caaagtcaag gagaagaaac gaatgcatca gatcaacaga atgaacccca gttaggcctg
      961 aaagaccagc aggttctaga tgtaaagagc tatgcacgtc ttttttcaaa gagcatcgag
     1021 actttgagag ttcatttagc agaaaagggg gatggagctg agctcatatg ggataaggat
     1081 gacccatctg caatggattt tgtcacctct gctgcaaacc tcaggatgca tattttcagt
     1141 atgaatatga agagtagatt tgatatcaaa tcaatggcag ggaacattat tcctgctatt
     1201 gctactacta atgcagtaat tgctgggttg atagtattgg aaggattgaa gattttatca
     1261 ggaaaaatag accagtgcag aacaattttt ttgaataaac aaccaaaccc aagaaagaag
     1321 cttcttgtgc cttgtgcact ggatcctccc aaccccaatt gttatgtatg tgccagcaag
     1381 ccagaggtga ctgtgcggct gaatgtccat aaagtgactg ttctcacctt acaagacaag
     1441 atagtgaaag aaaaatttgc tatggtagca ccagatgtcc aaattgaaga tgggaaagga
     1501 acaatcctaa tatcttccga agagggagag acggaagcta ataatcacaa gaagttgtca
     1561 gaatttggaa ttagaaatgg cagccggctt caagcagatg acttcctcca ggactatact
     1621 ttattgatca acatccttca tagtgaagac ctaggaaagg acgttgaatt tgaagttgtt
     1681 ggtgatgccc cggaaaaagt ggggcccaaa caagctgaag atgctgccaa aagcataacc
     1741 aatggcagtg atgatggagc tcagccctcc acctccacag ctcaagagca agatgacgtt
     1801 ctcatagttg attcggatga agaagattct tcaaataatg ccgacgtcag tgaagaagag
     1861 agaagccgca agaggaaatt agatgagaaa gagaatctca gtgcaaagag gtcacgtata
     1921 gaacagaagg aagagcttga tgatgtcata gcattagatc tcgacctgag aagccgtgct
     1981 caggcttcga actctgcagt tgacggcacc aacagcctta tcaaggagaa catgcgtatg
     2041 aaagttgtat tagaaggctc cgtcaatggg caccagttta aatgcacggg cgagggcgag
     2101 ggtaacccct atatgggaac tcagacgatg cgcattaaag ttattgaagg cggaccgtta
     2161 ccttttgctt ttgacatcct tgcaacttca ttcatgtatg gcagtaagac tttcatcaaa
     2221 tatccaaaag gaatccccga tttctttaag cagtcattcc ccgaaggctt cacgtgggag
     2281 cgtgtgactc gttacgagga tggtggggtt atcacggtaa tgcaagacac ctcacttgag
     2341 gacggctgct tggtttatca cgcgcaagtt cgcggcgtca attttccgag caacggggca
     2401 gtcatgcaaa agaaaacaaa ggggtgggaa cccaacactg agatgatgta ccccgcagat
     2461 ggggggttac gcggctataa tcacatggca ttgaaggttg atggcggggg ccacttatca
     2521 tgtagcctgg tcactacata ccgtagtaag aaaaccgtcg gaaatatcaa aatgccaggg
     2581 attcacgccg ttgaccgtcg tttagaacgc ttggaagagt cggacaatga gatgttcgtg
     2641 gtccaacgcg agcatgcagt ggctaaattt gccgggctgg gtggtgga
//
