LOCUS       sequence-339850-        2043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for glutamate, third generation, variant 857 in
            Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Aggarwal A, Liu R, Chen Y, Ralowicz AJ, Bergerson SJ, Tomaska F, 
            Mohar B, Hanson TL, Hasseman JP, Reep D, Tsegaye G, Yao P, Ji X, 
            Kloos M, Walpita D, Patel R, Mohr MA, Tillberg PW, Looger LL, Marvin
            JS, Hoppa MB, Konnerth A, Kleinfeld D, Schreiter ER, Podgorski K
  TITLE     Glutamate indicators with improved activation kinetics and 
            localization for imaging synaptic transmission.
  JOURNAL   Nat Methods. 2023 May 4. doi: 10.1038/s41592-023-01863-6.
  PUBMED    37142767
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 May 4. doi: 10.1038/s41592-023-01863-6."
COMMENT     Sequence Label: pAAV.hSyn.FLEX.iGluSnFR3.v857.SGZ.codonopt
FEATURES             Location/Qualifiers
     source          1..2043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             1639..1668
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1672..1818
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
ORIGIN
        1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
       61 gacggatccg ccgccggaag tactcttgat aagatcgcta aaaatggtgt aattgttgtc
      121 ggccaccgag actcttctgt gccattcagc tattatgatt ctcaacacaa agtggtgggc
      181 tattcacaag agtattctaa cgccattgtt gaagcagtta agaagaagtt gaataaacca
      241 gaccttcaag taaaactcat tcctataaca tcccaaaacc gcatacctct cttgcaaaac
      301 ggcacttacg atttcgagtg tgggagcact actaataatg tcgaacgcca gaaacaagcc
      361 gcattctccg acaccatctt cgtggtgggc actcgactgt tgacaaagaa aggcggtgat
      421 attaaggatt tcgcaaatct gaaggataag gcagtcgtgg tcaccagcgg gaccacctca
      481 gaagtactcc ttaataaact caatgaagaa caaaagatga atatgcgaat catctctgct
      541 aaggaccacg gtgactcttt tcgcacattg gaaagtgggc gcgccgtagc atttatgatg
      601 gacgactctc tgctcgctgg cgagcgcgcc aaagccaaaa aaccagacga ctgggaaatt
      661 gtaggtaaac ctcaaagcca agaggcatat ggctgcatgt tgagaaaaga tgacccccaa
      721 ttcaagaaac tgatggacga cactatagct caggtccaga cttcagggga agctgagaag
      781 tggtttgata aatggttcaa gaacccaatc ttggtaaatc ataccgttta tattaccgct
      841 gacaaacaaa agaatgggat aaaggcaaat ttcaagattc accataacgt agaggatgga
      901 agcgtgcagt tggcagacca ttatcaacag aataccccta ttggcgacgg acccgtgctc
      961 ctccctgaca accactactt gtcctatcaa agtgttctct caaaggatcc caatgagaag
     1021 cgagaccaca tggtacttct cgaattcgtt accgccaccg ggaattcact tggcatggat
     1081 gaattgtata aggggggtac tggcggcagc atgtccaagg gtgaggagct ttttaccgga
     1141 gtagtaccaa ttctcgttga acttgacgga gacgtcaacg ggcacaagtt tagcgtacga
     1201 ggtgaggggg agggtgatgc aaccaacggt aagcttactc tcaaattcat atgcactacc
     1261 ggaaagctgc cagtaccttg gccaacactg gtcactaccc tctcctatgg cgttcaatgt
     1321 tttgctcgat accctgacca catgaaacag catgacttct ttaaatcagc catgcctgaa
     1381 gggtatgtcc aggagcggac tatatcattt aaagacgatg gaacctataa gactcgggct
     1441 gaagtgaagc tcgagggtga cacattggtt aatcggatcg agcttaaggg catagatttt
     1501 aaagaagatg gtaatattct tggccacaaa ttggagtaca attttaataa tcctttgaac
     1561 atgaactttg agctctccga tgaaatgaag gcactcttta aagagccaaa cgacaaagct
     1621 ctgaaactgc aggtcgacga acaaaaactc atctcagaag aggatctgaa tgctgtgggc
     1681 caggacacgc aggaggtcat cgtggtgcca cactccttgc cctttaaggt ggtggtgatc
     1741 tcagccatcc tggccctggt ggtgctcacc atcatctccc ttatcatcct catcatgctt
     1801 tggcagaaga agccacgtga tagacacaag cagttgcgag ctacagcacg cgccaccgat
     1861 tatctgcaag ctgatgcaat aactagaatc cctgattata gatacagata tcaacgaagg
     1921 gatcgcgatg atgatcgaga tacagagcca gatcatgatc gggatgctga tcccgtcggg
     1981 gtgaaaggtt tcaacactct tccagataca gagatagata atccccagac gacttcagtg
     2041 tag
//
