LOCUS       sequence-339876-        8128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Hung YP, Albeck JG, Tantama M, Yellen G
  TITLE     Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded
            Fluorescent Biosensor.
  JOURNAL   Cell Metab. 2011 Oct 5;14(4):545-54.
  PUBMED    21982714
REFERENCE   2  (bases 1 to 8128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Metab."; date: "2011-10-5"; volume: "14(4)"; pages: "545-54"
COMMENT     Sequence Label: pcDNA3.1-Peredox-mCherry
FEATURES             Location/Qualifiers
     source          1..8128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..29)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        184..563
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     735..754
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        829..933
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(934..1002,1003..1794)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1152..1171)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1965..2553
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2454..2473
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2707..2724
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2841..2862
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2877..2882,2883..2900,2901..2907)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2915..2931
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2920..2942
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2939..2955
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2939..2955
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    3004..3125
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3015..3034)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3069..3088
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3299..4093)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3410..3429)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4020..4039
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4160..4489)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4174..4309
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4228..4247)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     4474..4494
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(4503..4931)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4613..4634)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4825..4844
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    4977..5201
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5190..5207
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(5240..5950)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5518..5537)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5787..5805
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(join(5951..5995,5996..6625,6626..6628,6629..7363,
                     7364..7384,7385..7996))
                     /codon_start=1
                     /product="genetically encoded fluorescent biosensor of the 
                     cytosolic NADH-NAD+ redox state (Hung et al., 2011)"
                     /label=Peredox
                     /note="consists of two pH-insensitive mutant copies of the 
                     Thermus aquaticus redox-sensing repressor Rex (T-Rex) 
                     separated by circularly permuted monomeric T-Sapphire 
                     (cpmTS), a pH-resistant GFP variant"
                     /translation="KVPEAAISRLITYLRILEELEAQGVHRTASEQLGELAQVTAFQVD
                     KDLSYFGSYGTDGVGYTVPVLKRELRHILGLNRKWGLCIVGMGRLGSALADWPGFGESF
                     ELRGFFDVDPGMVGRPVRGGVIEHVDLLPQRVPGRIEIALLTVPREAAQKAADLLVAAG
                     IKGILNFAPVVLEVPKEVAVENVDILAGLTRLSFAILNPTWSAAGGHGFTAHNVYIMAD
                     KQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSIQSKLSKDPNEK
                     RDHMVLLEFVTAAGITHGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFS
                     VSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVMVFARYPDHMKQHDFFKSA
                     MPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNTK
                     VPEAAISRLITYLRILEELEAQGVHRTASEQLGELAQVTAFQVDEDLSYFGSYGTDGVG
                     YTVPVLKRELRHILGLNRKWGLCIVGMGRLGSALADWPGFGESFELRGFFDVDPGMVGR
                     PVRGGVIEHVDLLPQRVPGRIEIALLTVPREAAQKAADLLVAAGIKGILNFAPVVLEVP
                     KEVAVENVDFLAGLTRLSFAILNPKWREEMMGSGTGGNASDGGGSGG"
     primer_bind     complement(8044..8063)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8045..8063)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(8108..8128,1..183))
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg
       61 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact
      121 tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat
      181 caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa
      241 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
      301 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc
      361 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
      421 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
      481 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
      541 ataactagtc aataatcaat gtcaacgcgt atatctggcc cgtacatcgc gaagcagcgc
      601 aaaacgccta accctaagca gattcttcat gcaattgtcg gtcaagcctt gccttgttgt
      661 agcttaaatt ttgctcgcgc actactcagc gacctccaac acacaagcag ggagcagata
      721 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata ggggatcggg
      781 agatctcccg atccgtcgac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
      841 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      901 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      961 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     1021 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     1081 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     1141 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     1201 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     1261 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1321 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1381 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     1441 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     1501 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     1561 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     1621 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     1681 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     1741 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     1801 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     1861 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1921 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1981 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2041 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2101 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2161 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2221 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2281 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2341 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2401 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2461 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2521 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2581 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     2641 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2701 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     2761 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     2821 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     2881 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     2941 ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacgg tatacagaca
     3001 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     3061 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     3121 aagttggggt gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg
     3181 gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt ggaatcgaaa
     3241 tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc
     3301 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
     3361 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
     3421 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
     3481 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
     3541 cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga
     3601 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
     3661 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
     3721 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
     3781 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
     3841 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
     3901 ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
     3961 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
     4021 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
     4081 cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatccg aaaatggata
     4141 tacaagctcc cgggagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
     4201 tctggaatag ctcagaggca gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     4261 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     4321 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     4381 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
     4441 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagaa
     4501 ttaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     4561 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     4621 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     4681 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     4741 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     4801 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     4861 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     4921 acagggcgcg tggggatacc ccctagagcc ccagctggtt ctttccgcct cagaagccat
     4981 agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc
     5041 ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc
     5101 ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg
     5161 ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg atcagcggtt
     5221 taaacttaag cttctcgact cacttgtaca gttcgtccat gccgccggtg gagtggcggc
     5281 cctcggcgcg ttcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca
     5341 acttgatgtt gacgttgtag gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg
     5401 tcttgacctc agcgtcgtag tggccgccgt ccttcagctt cagcctctgc ttgatctcgc
     5461 ccttcagggc gccgtcctcg gggtacatcc gctcggagga ggcctcccag cccatggtct
     5521 tcttctgcat tacggggccg tcggagggga agttggtgcc gcgcagcttc accttgtaga
     5581 tgaactcgcc gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga
     5641 agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcaag tagtcgggga
     5701 tgtcggcggg gtgcttcacg taggccttgg agccgtacat gaactgaggg gacaggatgt
     5761 cccaggcgaa gggcaggggg ccacccttgg tcaccttcag cttggcggtc tgggtgccct
     5821 cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct
     5881 ccatgtgcac cttgaagcgc atgaactcct tgatgatggc catgttatcc tcctcgccct
     5941 tgctcaccat accaccagac ccaccaccgt cagaggcgtt gcccccagtt ccggaaccca
     6001 tcatttcttc gcgccactta ggattcagga ttgcgaagct caggcgggtc aggccggcca
     6061 ggaaatcaac gttttcaaca gcaacttcct taggaacctc cagaacaaca ggggcgaagt
     6121 tcagaattcc cttgatacca gcggcgacta gtaagtcggc tgccttctgt gcagcttcgc
     6181 gaggaacggt cagcagggcg atctcgatgc gacccgggac gcgctgtggc aggaggtcaa
     6241 catgctcgat aacgccaccg cgaacagggc gcccaaccat gcctggatca acgtcgaaga
     6301 agccgcgcag ttcgaaagat tcaccaaagc ccggccagtc tgccagtgca ctaccgaggc
     6361 ggcccatgcc aacgatgcat aagccccact tgcggttcag gcccaggatg tggcgcagct
     6421 cgcgcttcag gacagggact gtgtagccaa caccgtcggt gccgtaggaa ccgaaatagg
     6481 acagatcttc gtcaacctgg aatgctgtaa cctgtgccag ttcgcccagc tgctcgcttg
     6541 cagtgcggtg aacgccttgg gcttcgagct cctccaggat gcggaggtaa gtgatgaggc
     6601 ggctgatggc ggcttcaggt accttggtgt tatattcaag cttatggccc aggatgttgc
     6661 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt aaccagagtg tcgccctcga
     6721 acttaacctc agcgcgtgtc ttatagttgc cgtcgtcctt gaaaaagatg gtgcgttcct
     6781 gaacgtagcc ctcaggcatg gcgctcttga agaagtcgtg ctgcttcatg tggtcaggat
     6841 atctggcgaa aaccataaca ccataggaga aggtagtaac cagggtaggc caaggaacag
     6901 gcagcttgcc agtggtgcag atgaacttca gagtcagctt gccgtaggta gcgtctcctt
     6961 cgccttcccc gctaacgctg aatttgtggc cgttaacgtc gccgtccagt tcaaccagga
     7021 taggaacaac accagtgaac agttcctcgc ctttggaaac catagatccc ccggtgccgc
     7081 ccttgtacag ttcgtccatg ccgtgggtga taccagcggc ggtaacaaat tccagcagaa
     7141 ccatgtggtc gcgcttctcg tttgggtctt tggacagttt agactggatg cttaagtagt
     7201 ggttgtcagg cagcagaaca gggccgtcgc cgattggggt gttctgctgg tagtgatcag
     7261 ccagctgaac gccgccgtct tcgatgttgt ggcggatctt gaagttggcc ttgataccgt
     7321 tcttctgttt gtcagccatg atatacacgt tgtgagcagt gaaaccatgc ccacctgctg
     7381 cgctccacgt ggggttcaga atggcaaagc tcagacgcgt cagaccggcc aggatgtcaa
     7441 cgttctcaac agccacttcc ttgggaacct ccagtacaac tggagcgaag ttcaggatgc
     7501 ccttgatgcc agcggcaacc agcaggtcgg cagccttctg tgctgcctcg cgtggaaccg
     7561 tcagcagagc gatttcgatg cgaccaggta cgcgctgggg cagcagatca acgtgttcga
     7621 taacaccacc gcgaaccggc cgaccaacca tacctgggtc aacatcgaag aaaccgcgca
     7681 gctcgaagct ctcgccaaaa ccaggccaat cagcaagagc ggatcccagg cggcccatcc
     7741 ccacgatgca caggccccat ttgcggttca gaccgaggat atggcgcagt tctctcttga
     7801 ggaccggtac agtgtagcca acgccgtcag tgccgtagga gccgaagtag gacaggtcct
     7861 tatcaacctg gaaggcggtg acctgggcca gctcgcccag ttgctcggag gcggtgcggt
     7921 gtacaccttg ggcttccagc tcttccagaa ttctcaggta agtaatcagt ctggaaatgg
     7981 ctgcttcagg aactttgcta gcaaccatgg tgggcccaat ctagagctag ccagcttggg
     8041 tctccctata gtgagtcgta ttaatttcga taagccagta agcagtgggt tctctagtta
     8101 gccagagagc tctgcttata tagacctc
//
