LOCUS       sequence-339914-        7336 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV packaging plasmid expressing Rep/Cap genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7336)
  TITLE     AAV packaging plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV2/rh10
FEATURES             Location/Qualifiers
     source          1..7336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     131..153
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     145..162
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     146..162
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     172..191
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        172..190
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     219..235
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     220..236
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4231..4242
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(4694..4714)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4694..4712)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4733..4749)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4733..4749)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4746..4768)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4757..4773
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4781..4787,4788..4805,4806..4811))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4826..4847
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4964..4981)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5135..5723)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5215..5234)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5894..6685,6686..6754))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6517..6536
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6755..6859)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(6885..7336,1..4))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7022..7043)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7234..7253
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
       61 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
      121 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
      181 tcactatagg gcgaattggg taccgggccc cccctcgatc gaggtcgacg gtatcggggg
      241 agctcgcagg gtctccattt tgaagcggga ggtttgaacg cgcagccgcc atgccggggt
      301 tttacgagat tgtgattaag gtccccagcg accttgacga gcatctgccc ggcatttctg
      361 acagctttgt gaactgggtg gccgagaagg aatgggagtt gccgccagat tctgacatgg
      421 atctgaatct gattgagcag gcacccctga ccgtggccga gaagctgcag cgcgactttc
      481 tgacggaatg gcgccgtgtg agtaaggccc cggaggctct tttctttgtg caatttgaga
      541 agggagagag ctacttccac atgcacgtgc tcgtggaaac caccggggtg aaatccatgg
      601 ttttgggacg tttcctgagt cagattcgcg aaaaactgat tcagagaatt taccgcggga
      661 tcgagccgac tttgccaaac tggttcgcgg tcacaaagac cagaaatggc gccggaggcg
      721 ggaacaaggt ggtggatgag tgctacatcc ccaattactt gctccccaaa acccagcctg
      781 agctccagtg ggcgtggact aatatggaac agtatttaag cgcctgtttg aatctcacgg
      841 agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc gcagacgcag gagcagaaca
      901 aagagaatca gaatcccaat tctgatgcgc cggtgatcag atcaaaaact tcagccaggt
      961 acatggagct ggtcgggtgg ctcgtggaca aggggattac ctcggagaag cagtggatcc
     1021 aggaggacca ggcctcatac atctccttca atgcggcctc caactcgcgg tcccaaatca
     1081 aggctgcctt ggacaatgcg ggaaagatta tgagcctgac taaaaccgcc cccgactacc
     1141 tggtgggcca gcagcccgtg gaggacattt ccagcaatcg gatttataaa attttggaac
     1201 taaacgggta cgatccccaa tatgcggctt ccgtctttct gggatgggcc acgaaaaagt
     1261 tcggcaagag gaacaccatc tggctgtttg ggcctgcaac taccgggaag accaacatcg
     1321 cggaggccat agcccacact gtgcccttct acgggtgcgt aaactggacc aatgagaact
     1381 ttcccttcaa cgactgtgtc gacaagatgg tgatctggtg ggaggagggg aagatgaccg
     1441 ccaaggtcgt ggagtcggcc aaagccattc tcggaggaag caaggtgcgc gtggaccaga
     1501 aatgcaagtc ctcggcccag atagacccga ctcccgtgat cgtcacctcc aacaccaaca
     1561 tgtgcgccgt gattgacggg aactcaacga ccttcgaaca ccagcagccg ttgcaagacc
     1621 ggatgttcaa atttgaactc acccgccgtc tggatcatga ctttgggaag gtcaccaagc
     1681 aggaagtcaa agactttttc cggtgggcaa aggatcacgt ggttgaggtg gagcatgaat
     1741 tctacgtcaa aaagggtgga gccaagaaaa gacccgcccc cagtgacgca gatataagtg
     1801 agcccaaacg ggtgcgcgag tcagttgcgc agccatcgac gtcagacgcg gaagcttcga
     1861 tcaactacgc agacaggtac caaaacaaat gttctcgtca cgtgggcatg aatctgatgc
     1921 tgtttccctg cagacaatgc gagagaatga atcagaattc aaatatctgc ttcactcacg
     1981 gacagaaaga ctgtttagag tgctttcccg tgtcagaatc tcaacccgtt tctgtcgtca
     2041 aaaaggcgta tcagaaactg tgctacattc atcatatcat gggaaaggtg ccagacgctt
     2101 gcactgcctg cgatctggtc aatgtggatt tggatgactg catctttgaa caataaatga
     2161 tttaaatcag gtatggctgc cgatggttat cttccagatt ggctcgagga caacctctct
     2221 gagggcattc gcgagtggtg ggacttgaaa cctggagccc cgaaacccaa agccaaccag
     2281 caaaagcagg acgacggccg gggtctggtg cttcctggct acaagtacct cggacccttc
     2341 aacggactcg acaaggggga gcccgtcaac gcggcggacg cagcggccct cgagcacgac
     2401 aaggcctacg accagcagct caaagcgggt gacaatccgt acctgcggta taaccacgcc
     2461 gacgccgagt ttcaggagcg tctgcaagaa gatacgtctt ttgggggcaa cctcgggcga
     2521 gcagtcttcc aggccaagaa gcgggttctc gaacctctcg gtctggttga ggaaggcgct
     2581 aagacggctc ctggaaagaa gagaccggta gagccatcac cccagcgttc tccagactcc
     2641 tctacgggca tcggcaagaa aggccagcag cccgcgaaaa agagactcaa ctttgggcag
     2701 actggcgact cagagtcagt gcccgaccct caaccaatcg gagaaccccc cgcaggcccc
     2761 tctggtctgg gatctggtac aatggctgca ggcggtggcg ctccaatggc agacaataac
     2821 gaaggcgccg acggagtggg tagttcctca ggaaattggc attgcgattc cacatggctg
     2881 ggcgacagag tcatcaccac cagcacccga acctgggccc tccccaccta caacaaccac
     2941 ctctacaagc aaatctccaa cgggacttcg ggaggaagca ccaacgacaa cacctacttc
     3001 ggctacagca ccccctgggg gtattttgac tttaacagat tccactgcca cttctcacca
     3061 cgtgactggc agcgactcat caacaacaac tggggattcc ggcccaagag actcaacttc
     3121 aagctcttca acatccaggt caaggaggtc acgcagaatg aaggcaccaa gaccatcgcc
     3181 aataacctta ccagcacgat tcaggtcttt acggactcgg aataccagct cccgtacgtc
     3241 ctcggctctg cgcaccaggg ctgcctgcct ccgttcccgg cggacgtctt catgattcct
     3301 cagtacgggt acctgactct gaacaatggc agtcaggccg tgggccgttc ctccttctac
     3361 tgcctggagt actttccttc tcaaatgctg agaacgggca acaactttga gttcagctac
     3421 cagtttgagg acgtgccttt tcacagcagc tacgcgcaca gccaaagcct ggaccggctg
     3481 atgaaccccc tcatcgacca gtacctgtac tacctgtctc ggactcagtc cacgggaggt
     3541 accgcaggaa ctcagcagtt gctattttct caggccgggc ctaataacat gtcggctcag
     3601 gccaaaaact ggctacccgg gccctgctac cggcagcaac gcgtctccac gacactgtcg
     3661 caaaataaca acagcaactt tgcctggacc ggtgccacca agtatcatct gaatggcaga
     3721 gactctctgg taaatcccgg tgtcgctatg gcaacccaca aggacgacga agagcgattt
     3781 tttccgtcca gcggagtctt aatgtttggg aaacagggag ctggaaaaga caacgtggac
     3841 tatagcagcg ttatgctaac cagtgaggaa gaaattaaaa ccaccaaccc agtggccaca
     3901 gaacagtacg gcgtggtggc cgataacctg caacagcaaa acgccgctcc tattgtaggg
     3961 gccgtcaaca gtcaaggagc cttacctggc atggtctggc agaaccggga cgtgtacctg
     4021 cagggtccta tctgggccaa gattcctcac acggacggaa actttcatcc ctcgccgctg
     4081 atgggaggct ttggactgaa acacccgcct cctcagatcc tgattaagaa tacacctgtt
     4141 cccgcggatc ctccaactac cttcagtcaa gctaagctgg cgtcgttcat cacgcagtac
     4201 agcaccggac aggtcagcgt ggaaattgaa tgggagctgc agaaagaaaa cagcaaacgc
     4261 tggaacccag agattcaata cacttccaac tactacaaat ctacaaatgt ggactttgct
     4321 gttaacacag atggcactta ttctgagcct cgccccatcg gcacccgtta cctcacccgt
     4381 aatctgtaat tgcttgttaa tcaataaacc ggttgattcg tttcagttga actttggtct
     4441 ctgcgaaggg cgaattcgtt taaacctgca ggactagagg tcctgtatta gaggtcacgt
     4501 gagtgttttg cgacattttg cgacaccatg tggtcacgct gggtatttaa gcccgagtga
     4561 gcacgcaggg tctccatttt gaagcgggag gtttgaacgc gcagccgcca agccgaattc
     4621 tgcagatatc catcacactg gcggccgctc gactagagcg gccgccaccg cggtggagct
     4681 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     4741 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     4801 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     4861 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     4921 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     4981 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     5041 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5101 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5161 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5221 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5281 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5341 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5401 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5461 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5521 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     5581 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     5641 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5701 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5761 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5821 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     5881 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5941 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     6001 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     6061 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     6121 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     6181 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     6241 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     6301 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     6361 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     6421 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     6481 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     6541 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     6601 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     6661 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     6721 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6781 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     6841 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa
     6901 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     6961 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     7021 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     7081 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     7141 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     7201 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     7261 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     7321 tgcgccgcta cagggc
//
