LOCUS       sequence-339997-        5005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 80 kD Penn State ladder protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Santilli RT, Williamson JE 3rd, Shibata Y, Sowers RP, Fleischman AN,
            Tan S
  TITLE     The Penn State Protein Ladder system for inexpensive protein 
            molecular weight markers.
  JOURNAL   Sci Rep. 2021 Aug 18;11(1):16703. doi: 10.1038/s41598-021-96051-x.
  PUBMED    34408191
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-08-18"; volume: "11(1):16703. doi"; pages: " 
            10.1038/s41598-021-96051-x"
COMMENT     Sequence Label: pPSU80
FEATURES             Location/Qualifiers
     source          1..5005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             152..175
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             185..214
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(2437..2455)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2451..2498
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2563..2654
                     /gene="bla"
                     /label=AmpR promoter
     terminator      3519..3558
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      3589..4177
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4078..4097
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4331..4348
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4363..4502
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4428..4447)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4588..4610
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4604..4795)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt
       61 tccctccggc tccggagagc tccaattgga attcgctagc tctagaaata attttgttta
      121 actttaagaa ggagatatac atatggcaag ctggtcacat ccgcagtttg aaaaaggtag
      181 cagtcatcat caccaccatc accatcatca tcacagcagc ggtagcggta gcgcagataa
      241 caaatttaac aaagaacagc agaacgcctt ctacgaaatt ctgcatctgc cgaatctgaa
      301 tgaagaacag cgtaatggtt ttatccagag cctgaaagat gatccgagcc agagcgcaaa
      361 tctgctggcc gaagcaaaaa aactgaatga tgcacaggca ccgaaaggat ctgctgataa
      421 caaatttaac aaagagcaac aaaacgcctt ttacgagatc ctgcatttac cgaacctgac
      481 cgaggaacag cgcaacggct ttattcagtc actgaaagac gacccgagtg tgagcaaaga
      541 aattctggca gaggccaaaa agctgaacga tgcccaagct ccgaaaggat ccggtagcag
      601 cggtagcagt ggttcaagcg caccggttcg tagcctgaat tgtaccctgc gtgatagcca
      661 gcagaaaagc ctggttatga gtggtccgta tgaactgaaa gcactgcatc tgcaaggtca
      721 ggatatggaa cagcaggttg tttttagcat gagctttgtt cagggtgaag aaagcaatga
      781 taaaattccg gttgcactgg gtctgaaaga gaaaaatctg tatctgagct gcgtgctgaa
      841 agatgataaa ccgaccttac agctggaaag cgttgatccg aaaaactacc cgaaaaagaa
      901 aatggaaaaa cgctttgtgt tcaacaaaat cgagatcaac aacaaacttg agtttgagag
      961 cgcacagttt ccgaattggt atattagcac cagccaggcc gaaaatatgc cggttttttt
     1021 aggtggcacc aaaggtggcc aggatattac cgattttacc atgcagtttg tgtccagcgc
     1081 cggcagaaag gccctgacta ataacaacaa tgccggtgaa gcggagcagc agccaccgaa
     1141 ggcgaagcgc gccagaatcg cttttcatct ggagctgccc aagcggagga ccgtgctcgg
     1201 caacgtgcta gtctgcggca acggcgatgt gggccaactt ggtctcggcg aggacatact
     1261 ggagcgcaag cgtctcagcc ctgtggcggg catcccggat gccgtggaca tatccgccgg
     1321 tggcatgcac aacctggtgc tgaccaagtc aggggacatt tactcattcg gttgcaatga
     1381 cgaaggtgct ctaggccgtg acaccagcga ggatggcagc gagtcaaagc cggatcttat
     1441 cgatttgccc ggcaaggctc tgtgcatttc ggcgggagac tcgcattccg cttgtctgct
     1501 ggaggatggc cgtgtcttcg cttggggctc gttccgggac tctcatggca acatgggcct
     1561 caccatcgat ggtaacaagc gtacgcccat cgatctgatg gagggcactg tctgttgtag
     1621 catcgcgtcc ggcgccgatc atttggtcat cctgaccact gccggaaagg tctttaccgt
     1681 gggctgcgct gagcagggac aactcgggcg gctgtcggag cgttccatct ccggagaggg
     1741 tcgtcgtggc aaacgcgact tgctgcggcc cacccagctc atcatcacgc gggccaaacc
     1801 ttttgaggcc atctgggcca ccaactactg cacattcatg cgcgagtcac agacacaggt
     1861 catctgggcc acagggctta acaacttcaa gcagctggcc cacgagacaa agggcaagga
     1921 gttcgcactg acgcccatca agacggaact caaagatatc cgacatattg ccgggggtca
     1981 gcaccatact gtcatcctga caacggacct taagtgttcc gtagtgggtc gtccggagta
     2041 cggtcggctt ggtctgggcg atgtcaagga tgttgtggag aagccaacga ttgtaaagaa
     2101 gctgaccgaa aagattgtgt ccgttggatg tggcgaagtc tgttcctatg ccgttacaat
     2161 tgatggcaaa ctgtattcct ggggctccgg tgtcaacaat caactaggcg ttggcgacgg
     2221 cgatgatgaa ctcgaaccga tcgtggttgt cagcaagaac acgcagggga agcacatgct
     2281 gctcgcatct ggcggtggtc agcacgcaat ctttttggtc aaagcggaca aacaggacca
     2341 gtaatgtaca agatccagat ctaagcttgg taccacgcgt gcgcgctgat ccggctgcta
     2401 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
     2461 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatcgg
     2521 gattagcccg cctaatgagc gggctttttt ttaattcccc tatttgttta tttttctaaa
     2581 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     2641 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     2701 cattttgcct tcctgttttt gctcacccag aaacgctcgt gaaagtaaaa gacgcagagg
     2761 accaattggg ggcacgagtg ggatacatag aactggactt gaatagcggt aaaatccttg
     2821 agagttttcg ccctgaagag cgttttccaa tgatgagcac tttcaaagtt ctgctatgtg
     2881 gagcagtatt atcccgtgta gatgcggggc aagagcaact cggacgacga atacactatt
     2941 cgcagaatga cttggttgaa tactccccag tgacagaaaa gcaccttacg gacggaatga
     3001 cggtaagaga attatgtagt gccgccataa cgatgagtga taacactgcg gcgaacttac
     3061 ttctgacaac catcggtgga ccgaaggaat taaccgcttt tttgcacaat atgggagacc
     3121 atgtaactcg ccttgaccgt tgggaaccag aactgaatga agccatacca aacgacgagc
     3181 gagacaccac aatgcctgcg gcaatggcaa caacattacg caaactatta actggcgaac
     3241 tacttactct ggcttcacgg caacaattaa tagactggct tgaagcggat aaagttgcag
     3301 gaccactact gcgttcggca cttcctgctg gctggtttat tgctgataaa tctggggcag
     3361 gagagcgtgg ttcacggggt atcattgccg cacttggacc agatggtaag ccttcccgta
     3421 tcgtagttat ctacacgacg ggtagtcagg caactatgga cgaacgaaat agacagattg
     3481 ctgaaatagg ggcttcactg attaagcatt ggtaaaccga tacaattaaa ggctcctttt
     3541 ggagcctttt tttttggacg gaccggtaga aaagatcaaa ggatcttctt gagatccttt
     3601 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3661 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3721 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3781 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3841 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3901 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3961 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4021 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4081 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4141 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4201 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     4261 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     4321 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
     4381 ccttacgcat ctgtgcggta tttcacaccg caatggtgca ctctcagtac aatctgctct
     4441 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
     4501 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     4561 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     4621 catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt
     4681 cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg
     4741 tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg
     4801 cctccgtgta agggggattt ctgttcatgg ggtaatgata ccgatgaaac gagagaggat
     4861 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa
     4921 acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg
     4981 cttcgttaat acagatgtag gtgtt
//
