LOCUS       sequence-340021-        2477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Part 1 of HexaPro-D614G spike to be used in the Spike 
            Display system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Javanmardi K, Chou CW, Terrace CI, Annapareddy A, Kaoud TS, Guo Q, 
            Lutgens J, Zorkic H, Horton AP, Gardner EC, Nguyen G, Boutz DR, 
            Goike J, Voss WN, Kuo HC, Dalby KN, Gollihar JD, Finkelstein IJ
  TITLE     Rapid characterization of spike variants via mammalian cell surface 
            display.
  JOURNAL   Mol Cell. 2021 Dec 16;81(24):5099-5111.e8. doi: 
            10.1016/j.molcel.2021.11.024.
  PUBMED    34919820
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.11"; journalName: "Mol Cell"; date: 
            "2021-12-16- 16"; volume: "81"; issue: "24"; pages: "5099-5111.e8"
COMMENT     Sequence Label: Spike Display_Part 1 Spacer
FEATURES             Location/Qualifiers
     source          1..2477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(641..675)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(746..1405)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1320..1339
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1587..2175
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2076..2095
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgtgacatgg ttccacgcca tccacgtgag cggcacaaac ggaaccaagc ggtttgataa
       61 ccccgtcctg ccattcaatg atggagttta cttcgccagt accgagaaga gtaacatcat
      121 ccggggctgg atcttcggca ccaccctgga tagcaaaaca cagagcctcc tgatcgtgaa
      181 caatgccacg aacgtcgtga tcaaggtgtg cgagttccag ttttgcaatg atcctttcct
      241 gggtgtgtac taccacaaga acaacaagag ctggatggaa agcgagttca gagtctacag
      301 cagcgccaac aactgcacat tcgagtacgt ctctcagcct tttctgatgg accttgaggg
      361 gaaacaaggc aacttcaaga acctgagaga attcgtgttc aagaacatcg acggctactt
      421 caaaatctac tccaagcaca cacccatcaa cctggtccgg gacctccctc agggcttcag
      481 cgccctggaa cccctggtcg acctgcccat aggcatcaac ataacgcggt tccaaaccct
      541 gctggccctg catagatcct acctgactcc tggcgacagc agcagcggat ggaccgccgg
      601 agctgcagcc tactatgtgg gtggcaggtg gaccagacca ataaaaaacg cccggcggca
      661 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
      721 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
      781 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
      841 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
      901 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
      961 gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     1021 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     1081 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     1141 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
     1201 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     1261 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     1321 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     1381 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     1441 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     1501 gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1561 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1621 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1681 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1741 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1801 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1861 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1921 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1981 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2041 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2101 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2161 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     2221 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg
     2281 cacctgccac ctgacatgtt cgtgttcctg gtgctcctgc ctctggtgag cagccagtgc
     2341 gtgaacctga ccacccgaac ccagctccca ccagcctaca ccaacagctt tacacggggc
     2401 gtgtactacc ctgacaaggt gttcagatct agcgtcctgc acagcactca ggacctcttc
     2461 ctgccgttct tcagcaa
//
