LOCUS       sequence-340135-        9080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 3xFLAG-tagged AMBRA1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Simoneschi D, Rona G, Zhou N, Jeong YT, Jiang S, Milletti G, Arbini 
            AA, O'Sullivan A, Wang AA, Nithikasem S, Keegan S, Siu Y, 
            Cianfanelli V, Maiani E, Nazio F, Cecconi F, Boccalatte F, Fenyo D, 
            Jones DR, Busino L, Pagano M
  TITLE     CRL4(AMBRA1) is a master regulator of D-type cyclins.
  JOURNAL   Nature. 2021 Apr;592(7856):789-793. doi: 10.1038/s41586-021-03445-y.
            Epub 2021 Apr 14.
  PUBMED    33854235
REFERENCE   2  (bases 1 to 9080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-021-03445-y"; journalName: "Nature"; date: 
            "2021-04"; volume: "592"; issue: "7856"; pages: "789-793"
COMMENT     Sequence Label: pcDNA3.1-AMBRA1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..9080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4543..4608
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(4633..4651)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4634..4651)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4671..4688)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4677..4901
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4947..5375
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5034..5053)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5244..5265
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5384..5404)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5389..5718
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5569..5704
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5631..5650
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5785..6579
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5839..5858)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6449..6468
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6753..6874
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6790..6809)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6844..6863
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6923..6939)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6923..6939)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6936..6958)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6947..6963
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6971..6977,6978..6995,6996..7001))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7016..7037
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7154..7171)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7325..7913)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7405..7424)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8084..8875,8876..8944))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8707..8726
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8945..9049)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgatatc
      901 gccaccatga aggttgtccc agaaaagaat gctgtccgga tactctgggg gcgagaacgg
      961 ggtgctcggg ccatgggagc tcagcggctt ctgcaggagc tggtagagga taaaacccgg
     1021 tggatgaaat gggagggcaa gagagtagaa ctgccggata gtccacgctc taccttctta
     1081 ttggccttca gcccagacag gactctctta gcctccaccc atgtgaacca taatatctat
     1141 attacggagg tgaagactgg caagtgtgtt cattccctga ttggacaccg ccgtactcca
     1201 tggtgtgtca cttttcatcc caccatctca ggccttattg cttctggctg cctagatggg
     1261 gaggttagga tttgggattt acacggtggc agtgaaagct ggttcacaga tagcaacaat
     1321 gccattgcct ccctggcttt ccaccctacg gctcagctcc tgctgattgc cactgccaat
     1381 gagatccact tctgggactg gagtcgacgg gaaccctttg ctgtggtgaa gacagctagt
     1441 gagatggaac gggtccgtct ggtgagattt gatccacttg gacactactt actcacagca
     1501 attgttaacc cctctaatca acagggtgat gacgaaccag agatccccat agatggaaca
     1561 gaattatccc actaccgtca gcgtgccctc ctgcaatcac agccagttcg ccggacgcct
     1621 ctcctccaca atttcctgca catgctgtcc tcccgctctt ctggcatcca gaccgagccc
     1681 ttccatcccc cggagcaggc ctcgtcaacg cagcaggacc agggcctcct gaaccggccg
     1741 tctgccttca gtacagtcca gagcagcact gccggcaaca cgctccgcaa cctcagtctg
     1801 ggtcctaccc gccgctcttt gggagggcct ctgtctagcc acccttctag gtatcaccga
     1861 gaaatagctc ctgggttgac aggatctgag tggacccgga cagtactcag tctgaactcc
     1921 cgctctgagg cggaatccat gcccccgccc agaaccagtg cctcttcggt gagtttgctg
     1981 tctgtgctga gacagcagga aggtggctct caggcatctg tgtacacttc agccacagaa
     2041 gggaggggtt ttccggcatc agggttggca actgagtcag atggagggaa tggctccagc
     2101 caaaacaact cgggcagcat tcgccatgag cttcagtgtg acctgagacg cttctttctg
     2161 gagtatgacc ggcttcagga gctggatcag agcctgagtg gggaagctcc ccagacccaa
     2221 caggcccagg aaatgctcaa caataacatt gaatctgaga ggccaggccc ttcccaccag
     2281 cccaccccac acagcagtga gaacaactcc aacctgtccc gtggccacct gaatcgctgt
     2341 cgtgcttgcc acaatctcct gaccttcaac aacgataccc tgcgctggga aagaaccaca
     2401 cctaactact cctctggcga ggctagttcc tcttggcagg tccccagctc ctttgagagt
     2461 gtgccatcaa gtggcagcca gttgccacct ctcgagcgga ctgagggcca aacgcccagc
     2521 tccagcaggc tggagttgag cagctctgct agtccgcagg aggagaggac tgtgggggtg
     2581 gcctttaacc aggagacagg ccactgggaa agaatttaca cccagtccag cagatctgga
     2641 actgtgtcac aggaggcctt acatcaggat atgcctgagg agagctctga ggaggattca
     2701 ctcaggagga ggctgctgga atcttccctc atttcattat cccgttatga tggagcagga
     2761 tccagagagc acccaattta cccagaccca gcgagattat ctcctgctgc atactacgcc
     2821 cagaggatga tccagtatct ctcacggaga gacagtattc gccagcgctc catgcgctac
     2881 caacagaacc gtctccgttc ttccacctcc tcctcttcct cagacaacca gggtccatca
     2941 gtagagggaa ccgacttgga atttgaggac tttgaggaca atggtgacag atccaggcac
     3001 cgagctccac gcaatgcccg gatgtctgca ccttcgcttg gacgctttgt cccaaggcgt
     3061 ttcttgctgc ctgagtactt gccttatgct gggatttttc atgaacgtgg acagcctggc
     3121 ttggctactc actcttctgt taacagggtc ctggcagggg cagtgatcgg tgatggacag
     3181 tctgctgtgg ccagtaacat tgccaatact acctaccggc tccagtggtg ggacttcact
     3241 aagtttgacc tccctgaaat cagtaatgct tccgtgaatg tgctggtgca gaactgcaag
     3301 atctacaatg atgccagctg tgacatttct gcagatggcc agctcctggc agctttcatc
     3361 cccagcagcc agaggggctt tcctgatgaa ggcatcctgg cagtgtactc cctggccccc
     3421 cataacctgg gcgaaatgct ctacaccaag cgatttggtc ccaatgccat ttcggtgagc
     3481 ctgtccccaa tgggcagata tgtaatggtg ggcttggcct cacgaaggat cctgctgcac
     3541 ccctccacag agcacatggt ggcccaggtc ttcaggctgc aacaggccca tggtggagag
     3601 acctccatga ggagagtttt caacgtcctt tatcccatgc ctgccgacca gcggagacat
     3661 gtcagtatca actctgcccg ttggctgcct gagccagggc ttggcttggc ctatggtact
     3721 aacaaaggag acctggtgat ctgccgacca gaggccttaa actctggtgt tgagtactac
     3781 tgggaccagc tgaacgagac ggtcttcact gtccattcca acagcaggag cagcgagcgg
     3841 cctggaacca gcagagccac atggaggaca gacagagaca tggggctgat gaatgccatt
     3901 gggcttcagc cccggaaccc tgccacctca gtgacatctc agggcaccga gactctggcc
     3961 cttcagctgc agaatgccga aacacagact gagagggagg tgccggagcc agggacagcc
     4021 gcctcaggtc ctggtgaagg tgagggttca gagtatggtg ccagtggaga agatgcgctc
     4081 agcaggatcc agaggctgat ggcggagggc ggcatgacag ccgtggtgca gcgggagcag
     4141 agcaccacca tggcctccat gggcggcttc ggcaacaaca tcatcgtcag ccaccgcatt
     4201 caccgcagct ctcagacggg cactgaacct ggtgccgccc acacctcctc accccagccc
     4261 tccacctctc ggggactgct cccagaggcc gggcaactgg cagagcgagg cctaagcccc
     4321 cggacagctt cctgggacca gcctggtacc cctgggcggg agccaaccca gccaaccctg
     4381 ccctcttcct cccctgtccc cattcctgtt tcccttccca gcgctgaggg accaaccgtc
     4441 cactgcgagt tgaccaataa caaccacctt ctggatggtg gcagcagcag gggggacgct
     4501 gcaggcccta ggggagaacc acggaacagg ggcggccgca tggactacaa ggaccacgac
     4561 ggcgactaca aggaccacga catcgactac aaggacgacg acgacaagta actcgagtct
     4621 agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt
     4681 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     4741 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     4801 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     4861 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     4921 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     4981 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5041 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5101 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5161 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5221 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5281 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5341 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     5401 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5461 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5521 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     5581 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5641 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5701 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     5761 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     5821 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     5881 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     5941 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     6001 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     6061 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     6121 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     6181 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     6241 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     6301 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6361 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     6421 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     6481 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     6541 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     6601 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     6661 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     6721 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     6781 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6841 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     6901 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6961 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7021 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7081 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7141 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7201 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7261 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7321 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7381 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7441 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7501 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7561 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7621 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7681 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7741 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7801 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7861 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7921 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7981 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8041 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8101 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8161 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8221 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8281 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8341 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8401 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8461 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8521 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8581 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8641 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8701 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8761 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8821 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8881 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8941 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9001 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9061 gaaaagtgcc acctgacgtc
//
