LOCUS       sequence-340290-        9416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to constitutively express TEAD-binding mutant YAP 
            under control of the EFS promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Pearson JD, Huang K, Pacal M, McCurdy SR, Lu S, Aubry A, Yu T, 
            Wadosky KM, Zhang L, Wang T, Gregorieff A, Ahmad M, Dimaras H, 
            Langille E, Cole SPC, Monnier PP, Lok BH, Tsao MS, Akeno N, Schramek
            D, Wikenheiser-Brokamp KA, Knudsen ES, Witkiewicz AK, Wrana JL, 
            Goodrich DW, Bremner R
  TITLE     Binary pan-cancer classes with distinct vulnerabilities defined by 
            pro- or anti-cancer YAP/TEAD activity.
  JOURNAL   Cancer Cell. 2021 Jul 13. pii: S1535-6108(21)00338-X. doi: 
            10.1016/j.ccell.2021.06.016.
  PUBMED    34270926
REFERENCE   2  (bases 1 to 9416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2021 Jul 13. pii: S1535-6108(21)00338-X. doi: 
            10.1016/j.ccell.2021.06.016."
COMMENT     Sequence Label: EFSp-GFP-YAP (S94A)
FEATURES             Location/Qualifiers
     source          1..9416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..232
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             280..345
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(1861..1885)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(1927..2501,2502..2504,2505..2513)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2094..2111)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2321..2340
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2514..2516,2517..2519,2520..3230)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2514..2516,2517..2519,2520..3230)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2559..2580)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2820..2839)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3167..3188
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3240..3293
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3294..3893
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3294..3313)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3790..3810
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4021..4254
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4326..4447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4363..4382)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4417..4436
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4487..4622
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4642..4661)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4643..4661)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4671..4688)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4671..4687)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4680..4702)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4829..5284
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4916..4935)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5126..5147
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5310..5414
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5415..5483,5484..6275)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5633..5652)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6446..7034
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6935..6954
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7188..7205
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7322..7343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7358..7363,7364..7381,7382..7388)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7396..7412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7401..7423
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7420..7436
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7420..7436
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7455..7475
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7457..7475
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7503..7729
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7730..7910
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7957..8082
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8575..8808
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8993..9037
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9250..9367
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
       61 gggaggggtc ggcaattgat ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      121 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      181 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agggaccgaa
      241 tcaccgacct ctctccccag ggggatccac cggagcatgg actacaaaga ccatgacggt
      301 gattataaag atcatgacat cgactacaag gatgacgatg acaagatgga tcccgggcag
      361 cagccgccgc ctcaaccggc cccccagggc caagggcagc cgccttcgca gcccccgcag
      421 gggcagggcc cgccgtccgg acccgggcaa ccggcacccg cggcgaccca ggcggcgccg
      481 caggcacccc ccgccgggca tcagatcgtg cacgtccgcg gggactcgga gaccgacctg
      541 gaggcgctct tcaacgccgt catgaacccc aagacggcca acgtgcccca gaccgtgccc
      601 atgaggctcc ggaagctgcc cgacgccttc ttcaagccgc cggagcccaa atcccactcc
      661 cgacaggcca gtactgatgc aggcactgca ggagccctga ctccacagca tgttcgagct
      721 cattcctctc cagcttctct gcagttggga gctgtttctc ctgggacact gacccccact
      781 ggagtagtct ctggcccagc agctacaccc acagctcagc atcttcgaca gtcttctttt
      841 gagatacctg atgatgtacc tctgccagca ggttgggaga tggcaaagac atcttctggt
      901 cagagatact tcttaaatca catcgatcag acaacaacat ggcaggaccc caggaaggcc
      961 atgctgtccc agatgaacgt cacagccccc accagtccac cagtgcagca gaatatgatg
     1021 aactcggctt caggtcctct tcctgatgga tgggaacaag ccatgactca ggatggagaa
     1081 atttactata taaaccataa gaacaagacc acctcttggc tagacccaag gcttgaccct
     1141 cgttttgcca tgaaccagag aatcagtcag agtgctccag tgaaacagcc accacccctg
     1201 gctccccaga gcccacaggg aggcgtcatg ggtggcagca actccaacca gcagcaacag
     1261 atgcgactgc agcaactgca gatggagaag gagaggctgc ggctgaaaca gcaagaactg
     1321 cttcggcagg caatgcggaa tatcaatccc agcacagcaa attctccaaa atgtcaggag
     1381 ttagccctgc gtagccagtt accaacactg gagcaggatg gtgggactca aaatccagtg
     1441 tcttctcccg ggatgtctca ggaattgaga acaatgacga ccaatagctc agatcctttc
     1501 cttaacagtg gcacctatca ctctcgagat gagagtacag acagtggact aagcatgagc
     1561 agctacagtg tccctcgaac cccagatgac ttcctgaaca gtgtggatga gatggataca
     1621 ggtgatacta tcaaccaaag caccctgccc tcacagcaga accgtttccc agactacctt
     1681 gaagccattc ctgggacaaa tgtggacctt ggaacactgg aaggagatgg aatgaacata
     1741 gaaggagagg agctgatgcc aagtctgcag gaagctttga gttctgacat ccttaatgac
     1801 atggagtctg ttttggctgc caccaagcta gataaagaaa gctttcttac atggttatag
     1861 acccagcttt cttgtacaaa gtggtgatct agctaaaacg cggcctcgaa tcgaattccg
     1921 cccccccccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     1981 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     2041 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     2101 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     2161 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     2221 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     2281 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     2341 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     2401 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     2461 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggtga
     2521 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     2581 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     2641 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     2701 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     2761 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     2821 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     2881 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     2941 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     3001 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     3061 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     3121 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     3181 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ggcagcggag
     3241 agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctgga cccatgaccg
     3301 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     3361 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     3421 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     3481 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     3541 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     3601 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     3661 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     3721 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     3781 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     3841 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
     3901 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg gtacctttaa
     3961 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     4021 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     4081 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     4141 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     4201 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     4261 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     4321 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     4381 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     4441 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     4501 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4561 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4621 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     4681 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     4741 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     4801 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     4861 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4921 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4981 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5041 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5101 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5161 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5221 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     5281 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5341 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5401 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     5461 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     5521 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     5581 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     5641 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     5701 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     5761 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5821 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     5881 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     5941 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6001 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6061 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6121 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6181 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6241 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6301 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6361 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6421 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6481 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6541 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6601 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6661 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6721 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6781 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6841 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6901 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6961 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7021 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7081 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7141 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7201 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     7261 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     7321 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     7381 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     7441 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     7501 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     7561 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     7621 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     7681 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     7741 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7801 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7861 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     7921 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     7981 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     8041 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     8101 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     8161 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     8221 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     8281 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     8341 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     8401 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     8461 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     8521 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     8581 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     8641 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     8701 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     8761 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     8821 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     8881 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     8941 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     9001 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     9061 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     9121 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     9181 tcagacccac ctcccaaccc cgaggggaca attctcgacc tcgagacaaa tggcagtatt
     9241 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     9301 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     9361 aaattttcgg gtttattaca gggacagcag agatccactt tggccgcggc tcgagg
//
