LOCUS       sequence-340306-        2074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty backbone for in vitro transcription of mRNA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2074)
  TITLE     Verstreken lab plasmids
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIVTR(T7G)-MCS
FEATURES             Location/Qualifiers
     source          1..2074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    65..121
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     rep_origin      complement(319..907)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(399..418)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1078..1869,1870..1938))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1701..1720
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1939..2043)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taatacgact cactataggg acatttgctt ctgacacaac tgtgttcact agcaacctca
       61 aacagaattc gagctcggta cccggggatc ctctagagtc gacctgcagg catgcaagct
      121 tgctcgcttt cttgctgtcc aatttctatt aaaggttcct ttgttcccta agtccaacta
      181 ctaaactggg ggatattatg aagggccttg agcatctgga ttctgcctaa taaaaaacat
      241 ttattttcat tgcaaaacca tgggagcaaa aggccagcaa aaggccagga accgtaaaaa
      301 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      361 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      421 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      481 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      541 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      601 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      661 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
      721 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
      781 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
      841 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      901 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
      961 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     1021 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     1081 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     1141 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     1201 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     1261 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     1321 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     1381 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     1441 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     1501 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     1561 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     1621 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     1681 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     1741 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     1801 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     1861 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     1921 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     1981 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     2041 gcgcacattt ccccgaaaag tgccacctga cgtc
//
