LOCUS       sequence-340516-       12571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes OptoAMP1 circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12571)
  AUTHORS   Zhao EM, Lalwani MA, Chen JM, Orillac P, Toettcher JE, Avalos JL
  TITLE     Optogenetic Amplification Circuits for Light-Induced Metabolic 
            Control.
  JOURNAL   ACS Synth Biol. 2021 May 21;10(5):1143-1154. doi: 
            10.1021/acssynbio.0c00642. Epub 2021 Apr 9.
  PUBMED    33835777
REFERENCE   2  (bases 1 to 12571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00642"; journalName: "ACS Synth Biol"; date: 
            "2021-05-21- 21"; volume: "10"; issue: "5"; pages: "1143-1154"
COMMENT     Sequence Label: EZ-L175
FEATURES             Location/Qualifiers
     source          1..12571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(73..104)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     terminator      complement(275..522)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     488..506
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(1411..1431)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2838..2857)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2839..2857)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2864..2881)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2864..2880)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2873..2895)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3021..3476
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3108..3127)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3318..3339
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3832..3851)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3951..3973
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4011..4029)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4113..4217
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4218..4286,4287..5078)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4436..4455)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5249..5837
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5738..5757
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5991..6008
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6125..6146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6161..6166,6167..6184,6185..6191)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6199..6215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6204..6226
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6223..6239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6223..6239
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        6260..6278
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6604..6791
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     terminator      complement(8254..8419)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     8340..8364
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(8435..9142)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(8475..8495)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     9090..9118
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     promoter        complement(9184..9625)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(9190..9213)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    9508..9625
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     CDS             complement(join(9945..12571,1..16))
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
ORIGIN
        1 aagacagtag cttcatgcta gccatgattt tagcggccgc ggtggcttta ccaacagtac
       61 cggattgcca agctggaagt cgagcttcca ttatataccc tctagtgtct cttaagcatg
      121 gactaaaggc tacctataac gcatggacta aaggctacct ataacgcatg gactaaaggc
      181 tacctataac gcatggacta aaggctacct ataacgcatg gactaaaggc tacctactcg
      241 aagctggcga actcacgtac ccggcgataa ggccgcaaat taaagccttc gagcgtccca
      301 aaaccttctc aagcaaggtt ttcagtataa tgttacatgc gtacacgcgt ctgtacagaa
      361 aaaaaagaaa aatttgaaat ataaataacg ttcttaatac taacataact ataaaaaaat
      421 aaatagggac ctagacttca ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg
      481 ggggagggcg tgaatgtaag cgtgacataa ctaattacat gatcattact cgaggtcgac
      541 ttaaatacct gcttctacgg caattctaac taaatctgca ctcgttttta agtttaattt
      601 ttccattacc aaacctctgt gcattttgac agttttttct gaaagaccca gtctggcagc
      661 cacttcttta tttcttaggc ctgatgcaac cagagttgtg acttctaatt gacgtggtga
      721 aagagttttt aacatctccg cagcacgttc tctacgggcc atgcccatgt tcggttgatc
      781 gtcatccact tccacttggc taccaaggaa gtatagtaat tcatcatcgt catcatagat
      841 tggtgccacc aaaactgcat tacgaaaagg agtaccatcc tttttgtaat tcaaaatctc
      901 aaccaacacg ggtttatgct cacgaacacc ttgacggatc ttatctgtta gccagggttc
      961 agtaccagat cctgccaaaa atctacaatt acgaccaaca cattcttcct cagaataccc
     1021 agttagatcg gtaaaggcct ggttgatagc aatcaaagga ttatcagcca atctgggatc
     1081 actaacaaca cttgctatgg gtgaggcctc gatcaaatcc aatacccatt gggctggagg
     1141 ttggacttca actctagtat catctgctcc gaattcacct ccatattcgt cgataccaag
     1201 tgcatcagta aacatctgtt caaattcgaa atctgccatg tccaacgcgc catacggagc
     1261 agaatcatgt ggtgtaaatc cggggccggg agaatcccca tcacctaaca tgtccagatc
     1321 aaaatcatca agagcatcgg catgtgccat agctacatcc tcaccgtcta aatgtaattc
     1381 gtcaccaagt gaaacgtccg ttggtggggc tacttttctc ttttttttcg ggctagccat
     1441 gattttagcg gccgccaccg cggtgtttta tatttgttgt aaaaagtaga taattacttc
     1501 cttgatgatc tgtaaaaaag agaaaaagaa agcatctaag aacttgaaaa actacgaatt
     1561 agaaaagacc aaatatgtat ttcttgcatt gaccaattta tgcaagttta tatatatgta
     1621 aatgtaagtt tcacgaggtt ctactaaact aaaccacccc cttggttaga agaaaagagt
     1681 gtgtgagaac aggctgttgt tgtcacacga ttcggacaat tctgtttgaa agagagagag
     1741 taacagtacg atcgaacgaa ctttgctctg gagatcacag tgggcatcat agcatgtggt
     1801 actaaaccct ttcccgccat tccagaacct tcgattgctt gttacaaaac ctgtgagccg
     1861 tcgctaggac cttgttgtgt gacgaaattg gaagctgcaa tcaataggaa gacaggaagt
     1921 cgagcgtgtc tgggtttttt cagttttgtt ctttttgcaa acaaatcacg agcgacggta
     1981 atttctttct cgataagagg ccacgtgctt tatgagggta acatcaattc aagaaggagg
     2041 gaaacacttc ctttttctgg ccctgataat agtatgaggg tgaagccaaa ataaaggatt
     2101 cgcgcccaaa tcggcatctt taaatgcagg tatgcgatag ttcctcactc tttccttact
     2161 cacgagtaat tcttgcagct gtttcccggg agctggaact cccacctgca acatgcggga
     2221 tcctgagtaa ataagcggcc gccaccgcgg tggagctcag atcttgacac cgattattta
     2281 aagctgcagc atacgatata tatacatgtg tatatatgta tacctatgaa tgtcagtaag
     2341 tatgtatacg aacagtatga tactgaagat gacaaggtaa tgcatcattc tatacgtgtc
     2401 attctgaacg aggcgcgctt tccttttttc tttttgcttt ttcttttttt ttctcttgaa
     2461 ctccagaaaa aaaatataaa agagatggag gaacgggaaa aagttagttg tggtgatagg
     2521 tggcaagtgg tattccgtaa gaacaacaag aaaagcattt catattatgg ctgaactgag
     2581 cgaacaagtg caaaatttaa gcatcaacga caacaacgag aatggttatg ttcctcctca
     2641 cttaagagga aaaccaagaa gtgccagaaa taacagtagc aactacaata acaacaacgg
     2701 cggctacaac ggtggccgtg gcggtggcag cttctttagc aacaaccgtc gtggtggtta
     2761 cggcaacggt ggtttcttcg gtggaaacaa cggtggcagc ggccggccat ttaaatgttt
     2821 aaacggccac gctggccccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
     2881 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     2941 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     3001 gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     3061 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     3121 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     3181 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     3241 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     3301 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     3361 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     3421 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg
     3481 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatagg gtaataactg
     3541 atataattaa attgaagctc taatttgtga gtttagtatt aggatctcta cccttggcga
     3601 aaagtcctct gccaacaatg atgatatctg atccaccact tacaacttcg tcgagggttc
     3661 tgtactgctg acccaatatg cgtatatata ccaatctaag tctgtgctcc ttccttcgtt
     3721 cttccttctg ttcggagatt accgaatcaa aaaaatttca aggaaaccga aatcaaaaaa
     3781 aagaataaaa aaaaaatgat gaattgaaaa ggtggtatgg tgcactctca gtacaatctg
     3841 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     3901 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     3961 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     4021 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt cacgcgccga
     4081 ggacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4141 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4201 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4261 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4321 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     4381 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     4441 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     4501 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     4561 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     4621 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     4681 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     4741 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     4801 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     4861 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     4921 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     4981 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5041 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5101 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5161 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5221 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5281 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5341 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5401 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5461 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5521 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5581 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5641 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5701 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5761 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5821 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5881 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     5941 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6001 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     6061 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     6121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     6181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     6241 atgattacgc caagctcgga attaaccctc actaaaggga acaaaagctg ggccggccat
     6301 ttaaatgttt aaacggccac gctggccgct gcacggtcct gttccctagc atgtacgtga
     6361 gcgtatttcc ttttaaacca cgacgctttg tcttcattca acgtttccca ttgttttttt
     6421 ctactattgc tttgctgtgg gaaaaactta tcgaaagatg acgacttttt cttaattctc
     6481 gttttaagag cttggtgagc gctaggagtc actgccaggt atcgtttgaa cacggcatta
     6541 gtcagggaag tcataacaca gtcctttccc gcaattttct ttttctatta ctcttggcct
     6601 cctctagtac actctatatt tttttatgcc tcggtaatga ttttcatttt tttttttcca
     6661 cctagcggat gactcttttt ttttcttagc gattggcatt atcacataat gaattataca
     6721 ttatataaag taatgtgatt tcttcgaaga atatactaaa aaatgagcag gcaagataaa
     6781 cgaaggcaaa gaggctctat atgtatataa agctgcggat gccttctgat ttaatctagt
     6841 tctagtttac ttcaggttta cttaaagttg agataattga agcaaagaga agcatcgagc
     6901 aaaggatggc gtttgttaag agggttacgc aggagacgaa tatacagctg gcgctggatc
     6961 ttgacggtgg gtctgtttct gtacgggaga gcatactggg caaggaatat gctagtggtg
     7021 atgggcagac catccatgtg cacactggag ttgggttttt ggaccacatg ttgactgcgc
     7081 tggcgaagca tggcgggtgg tctctgatcc tggagtgtat aggggacttg cacatcgatg
     7141 accaccacac tgttgaggac tgtgggattg cgctgggcca agcgttcaag gaggcgcttg
     7201 gctccgtgcg tggtatcaag aggttcgggc atgggtttgc accactggac gaggcgctga
     7261 gccgcgctgt ggttgacttc tccaataggc ctttcgccgt ggtggagctg ggcctgaagc
     7321 gagaacgcat aggccagcta tccacagaga tgatcccgca cttcttggag agtttcgcca
     7381 ctgaggcgcg tatcaccatg catgtggact gtctgcgggg caccaacgac caccaccgct
     7441 ccgaatcagc tttcaaggcg ctcgccatcg ccatcagaga ggcaagaaca cctacgggtc
     7501 gcgatgacgt tccatccact aagggtgtcc tagcatagaa cacagcccac agctaccacc
     7561 atcaacaata tttatataga taacgtacac atagaaatca cacaaacaga gtatttattc
     7621 ttaactacat gaactaccat cagaccgtct gggccactat ataatgtgct attcataaac
     7681 gtgatcactc tacgtagcag gcgaccccag gtgaaaattt ttcagcgagc tgccagattg
     7741 tcaggtgaaa aactgaaaaa aacttctggg cgatgagctt gtggcgggaa attaagtata
     7801 taaagcagtt agtttcttct gcttcttgtg gttctgggta ttcttgtaat atttggaata
     7861 atagaaaacc aagactactg cctttctttt catattatag aggaagaaat acgcacgaac
     7921 acgatataga ggtaaatttt ttgcaaaaag ataccaaact ataaaagaaa tagaagacta
     7981 aactacaaaa aataaagaaa gtaatacata tatccaatat gactgagtta gcagagcaag
     8041 tttccatgcc tgaagggcaa caaagacgtg gtggttacgt tccacctcac atccgtaacg
     8101 gtggtggcag aagaggcgat tctgctcctc gtggtggttt caacaacggt ggcggccgtg
     8161 gtggtttctt cagaaacaac ggtgaccgtg gtggattcgg ctttgggtac ctccggatat
     8221 gttggcgcgc cttaatttat tcgccggcga taagagcgac ctcatgctat acctgagaaa
     8281 gcaacctgac ctacaggaaa gagttactca agaataagaa ttttcgtttt aaaacctaag
     8341 agtcacttta aaatttgtat acacttattt tttttataac ttatttaata ataaaaatca
     8401 taaatcataa gaaattcgct gatcattact cgagtttgta tagttcatcc atgccatgtg
     8461 taatcccagc agctgttaca aactcaagaa ggaccatgtg gtctctcttt tcgttgggat
     8521 ctttcgaaag ggcagattgt gtggacaggt aatggttgtc tggtaaaagg acagggccat
     8581 cgccaattgg agtattttgt tgataatggt ctgctagttg aacgcttcca tcttcaatgt
     8641 tgtgtctaat tttgaagtta actttgattc cattcttttg tttgtctgcc atgatgtata
     8701 cattgtgtga gttatagttg tattccaatt tgtgtccaag aatgtttcca tcttctttaa
     8761 aatcaatacc ttttaactcg attctattaa caagggtatc accttcaaac ttgacttcag
     8821 cacgtgtctt gtagttcccg tcatctttga aaaatatagt tctttcctgt acataacctt
     8881 cgggcatggc actcttgaaa aagtcatgcc gtttcatatg atctgggtat cttgaaaagc
     8941 attgaacacc ataagtgaaa gtagtgacaa gtgttggcca tggaacaggt agttttccag
     9001 tagtgcaaat aaatttaagg gtaagttttc cgtatgttgc atcaccttca ccctctccac
     9061 tgacagaaaa tttgtgccca ttaacatcac catctaattc aacaagaatt gggacaactc
     9121 cagtgaaaag ttcttctcct ttgtcgacgc tagccatgat tttagcggcc gctatagttt
     9181 tttctccttg acgttaaagt atagaggtat attaacaatt ttttgttgat acttttatga
     9241 catttgaata agaagtaata caaaccgaaa atgttgaaag tattagttaa agtggttatg
     9301 cagcttttgc atttatatat ctgttaatag atcaaaaatc atcgcttcgc tgattaatta
     9361 ccccagaaat aaggctaaaa aactaatcgc attattatcc tatggttgtt aatttgattc
     9421 gttgatttga aggtttgtgg ggccaggtta ctgccaattt ttcctcttca taaccataaa
     9481 agctagtatt gtagaatctt tattgttcgg agcagtgcgg cgcgaggcac atctgcgttt
     9541 caggaacgcg accggtgaag accaggacgc acggaggaga gtcttccgtc ggagggctgt
     9601 cgcccgctcg gcggcttcta atccgcccgg cgataaatat gatacacggt ccaatggata
     9661 aacatttttt atcaacacta tgatatataa atataatagt ttttcgtata tatattcctt
     9721 tttttggtca atttttgaaa ttttcgtaga aaagggagag acaaaacaca ttatatcaat
     9781 gaaaacgtac aaaaagtaga taaagtcagt gcttaaacac gtcttttcct taaaaatact
     9841 ttattatttt tattttattg agagggtggt ttaaaaatag aaatagagag agaggtacat
     9901 acataaacat acgcgcacaa aagcagagat gatcattact cgagctcttt ttttgggttt
     9961 ggtggggtat cttcatcatc gaatagatag ttatatacat catccattgt agtggtatta
    10021 aacatccctg tagtgattcc aaacgcgtta tacgcagttt ggtccgtcca accaggtgac
    10081 agtggttttg aattattacc atcatcaatt ttactagccg tgatttcatt attcatgaag
    10141 ttatcatgaa cgttagagga ggcaattggt tgtgaaagcg cttgagaatt tgtttgagtt
    10201 gttatgaggt tcggaccgtt gctactgtta gtgaaagtga aggacaatga gctatcagca
    10261 atattcccac tttgattaaa attggcgcca ccaaacaaag cagacggggt cagtggcact
    10321 aatgattgca gctgttgctg ttgccctaga aaaggcgtga ctgagcgatg cgaaggtgtg
    10381 cttcttggta ttgtcactgg agagttacga gagggtggac ggttagataa cagcttgact
    10441 agatcactga aacttgctcc tgatttcaat ggcacaggtg aaggccctac tgagccagga
    10501 gaaacatatt taacactgat attgttgaca ttttcctccg gaagagtagg gtattgggcg
    10561 atagttgcag aaccgacaat atttttaatg gcgctaccat tactattgtt ataactgata
    10621 tgcggtaatg ggattgcaca ctgtgataac agaaacggcg cacatacctc ttccagtact
    10681 tgaatgtatt tttcacaagt ctggatttta aaagtggcca gtttttttaa tagcatcaga
    10741 acagtgttaa tttgttgtaa taattgtgcg gtctcgttat tctcagcatt cgattttgag
    10801 tttgagagta gagtctttat gggtactagg actgcattga acaagtaata agaacaattc
    10861 caggcaaaat atggggtgac attatgattg tccatatagc tacttacaga cataacagtt
    10921 ctttgtgctg catcgcttaa catgatggag catcgtttaa cttcataact ttgatgatca
    10981 ttttgatcct gttctagttg tgactttttc tgggtaaaat tagtgaaaaa atctcttaat
    11041 acataaatga taagagacaa ctgtttccac ttcagttcga atcttgtaaa ggatagccaa
    11101 gggtgttcct tcaacaaatt ggttagagcg gtggtggaaa tatccatttg taaaaacttt
    11161 ggtgcctgtc tcgaaacctc ctcaatctca ttacaaatca tcaagcattt ttttgcacat
    11221 ataggacttt tttctgcagt tactgttttg tctagttcat agatttttgt gaaaacttgt
    11281 aagagccttg ctgtttcaat gatgccatga tatatggtgg gacctgttgt ggtacgctgc
    11341 acatcgtcga cagaagaagg gaaggagatt gtattctgag aaagctggat ggatcgacca
    11401 taaagcaggg acaattggat ctcccaagag tagacagacc accaaattcg gcgtctttgt
    11461 tccagaatgc tgctatcact gaaggacgag gggaggtccc tattcaagcc caatgatatg
    11521 gccattctta tggaaaagct gtgaaaatta tagctagtat ttgttttctg cctccactgt
    11581 gtatatcgcg acagaagatg tagggctgtc accaaaatta tggaacctga ctcgaagacc
    11641 ttgctcgtca aatgagattt agcattttga tagtaaaaaa catctatatc agtagattcc
    11701 ccctctatac accaggctcc aatggctaat atgcagttaa aaaggatttg ccattgatcc
    11761 ttcgacgcga tttcaatctg gttattatac aacatcatta gcgtcggtga gtgcacgata
    11821 gggcagtagg ggtgaaaatt attgagataa ctttgaagta aacgggatgt tgtggatcta
    11881 gaagccaacg tgtatctatc cgtaatcatg gtcgggagcc tgttaacgtt agagttcgtg
    11941 taattttccg gtttaaagcc aatagatcga agaatacata agagagaacc gtcgccaaag
    12001 aacccattat tgttggggtc cgttttcagg aagggcaagc catccgacat gtcatcctct
    12061 tcagaccaat caaatccatg aagagcatcc ctgggcataa aatccaacgg aattgtggag
    12121 ttatcatgat gagctgccga gtcaatcgat acagtcaact gtctttgacc tttgttacta
    12181 ctctcttccg atgatgatgt cgcacttatt ctatgctgtc tcaatgttag aggcatatca
    12241 gtctccactg aagccaatct atctgtgacg gcatctttat tcacattatc ttgtacaaat
    12301 aatcctgtta acaatgcttt tatatcctgt aaagaatcca ttttcaaaat catgtcaagg
    12361 tcttctcgag gaaaaatcag tagaaatagc tgttccagtc tttctagcct tgattccact
    12421 tctgtcagat gtgccctagt cagcggagac cttttggttt tgggagagta gcgacactcc
    12481 cagttgttct tcagacactt ggcgcacttc ggtttttctt tggagcactt gagcttttta
    12541 agtcggcaaa tatcgcatgc ttgttcgata g
//
