LOCUS       sequence-340538-        9033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses microID in mammalian cells with the tet-on or tet-off 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9033)
  AUTHORS   Kubitz L, Bitsch S, Zhao X, Schmitt K, Deweid L, Roehrig A, 
            Barazzone EC, Valerius O, Kolmar H, Bethune J
  TITLE     Engineering of ultraID, a compact and hyperactive enzyme for 
            proximity-dependent biotinylation in living cells.
  JOURNAL   Commun Biol. 2022 Jul 4;5(1):657. doi: 10.1038/s42003-022-03604-5.
  PUBMED    35788163
REFERENCE   2  (bases 1 to 9033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-07-4"; volume: "5(1):657. doi"; pages: " 
            10.1038/s42003-022-03604-5"
COMMENT     Sequence Label: pSF3-microID
FEATURES             Location/Qualifiers
     source          1..9033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             519..988
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1053..1410
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     1821..1843
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1859..1875
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    1928..1961
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     polyA_signal    2069..2203
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2106..2125)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2391..4043)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     3959..3977
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        complement(4142..4179)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(4196..4214,4215..4237,4238..4256,4257..4279,4280..4298,
                     4299..4321,4322..4340,4341..4363,4364..4382,4383..4405,
                     4406..4424,4425..4447,4448..4466)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        4481..4519
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4627..4656
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    5275..5322
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(6406..6423)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6406..6422)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6415..6437)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6631..6650)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6750..6772
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6810..6828)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6896..7000
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7001..7069,7070..7861)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7219..7238)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8032..8620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8521..8540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8774..8791
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8908..8929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8944..8949,8950..8967,8968..8974)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8982..8998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8987..9009
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9006..9022
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9006..9022
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 caagctagct ttgctcttag gagtttccta atacatccca aactcaaata tataaagcat
       61 ttgacttgtt ctatgcccta gggggcgggg ggaagctaag ccagcttttt ttaacattta
      121 aaatgttaat tccattttaa atgcacagat gtttttattt cataagggtt tcaatgtgca
      181 tgaatgctgc aatattcctg ttaccaaagc tagtataaat aaaaatagat aaacgtggaa
      241 attacttaga gtttctgtca ttaacgtttc cttcctcagt tgacaacata aatgcgctgc
      301 tgagaagcca gtttgcatct gtcaggatca atttcccatt atgccagtca tattaattac
      361 tagtcaatta gttgattttt atttttgaca tatacatgtg aaagacccca cctgtaggtt
      421 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat
      481 agaaaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat
      541 atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata
      601 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
      661 tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg
      721 gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc
      781 tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac
      841 tcggcgcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc
      901 tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga
      961 ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg ggagacccct
     1021 gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta tctgtgtctg
     1081 tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta gttagctaac
     1141 tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc cggccgcaac
     1201 cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg agtcctaaaa
     1261 tcccgatcgt ttaggactct ttggtgcacc ccccttagag gagggatatg tggttctggt
     1321 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggga
     1381 ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc tctgtctgac
     1441 tgtgtttctg tatttgtctg aaaatatggg cccgggctag cctgttacca ctcccttaag
     1501 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1561 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1621 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1681 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1741 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     1801 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     1861 ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga tccgaagttc
     1921 ctattccgaa gttcctattc ttcaaatagt ataggaactt cagatcataa tcagccatac
     1981 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     2041 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     2101 ataaggcaat agcatcacaa atttcacaaa taaggcattt ttttcactgc attctagttt
     2161 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctcaaatc cctcggaagc
     2221 tgcgcctgtc ttaggttgga gtgatacatt tttatcactt ttacccgtct ttggattagg
     2281 cagtagctct gacggccctc ctgtcttagg ttagtgaaaa atgtcactct cttacccgtc
     2341 attggctgtc cagcttagct cgcaggggag gtggtctgga tcctctagaa ttacacggcg
     2401 atctttccgc ccttcttggc ctttatgagg atctctctga tttttcttgc gtcgagtttt
     2461 ccggtaagac ctttcggtac ttcgtccaca aacacaactc ctccgcgcaa ctttttcgcg
     2521 gttgttactt gactggcgac gtaatccacg atctcttttt ccgtcatcgt ctttccgtgc
     2581 tccaaaacaa caacggcggc gggaagttca ccggcgtcat cgtcgggaag acctgcgaca
     2641 cctgcgtcga agatgttggg gtgttggagc aagatggatt ccaattcagc gggagccacc
     2701 tgatagcctt tgtacttaat cagagacttc aggcggtcaa cgatgaagaa gtgttcgtct
     2761 tcgtcccagt aagctatgtc tccagaatgt agccatccat ccttgtcaat caaggcgttg
     2821 gtcgcttccg gattgtttac ataaccggac ataatcatag gacctctcac acacagttcg
     2881 cctctttgat taacgcccag cgttttcccg gtatccagat ccacaacctt cgcttcaaaa
     2941 aatggaacaa ctttaccgac cgcgcccggt ttatcatccc cctcgggtgt aatcagaata
     3001 gctgatgtag tctcagtgag cccatatcct tgcctgatac ctggcagatg gaacctcttg
     3061 gcaaccgctt ccccgacttc cttagagagg ggagcgccac cagaagcaat ttcgtgtaaa
     3121 ttagataaat cgtatttgtc aatcagagtg cttttggcga agaaggagaa tagggttggc
     3181 accagcagcg cactttgaat cttgtaatcc tgaaggctcc tcagaaacag ctcttcttca
     3241 aatctataca ttaagacgac tcgaaatcca catatcaaat atccgagtgt agtaaacatt
     3301 ccaaaaccgt gatggaatgg aacaacactt aaaatcgcag tatccggaat gatttgattg
     3361 ccaaaaatag gatctctggc atgcgagaat ctcacgcagg cagttctatg aggcagagcg
     3421 acacctttag gcagaccagt agatccagag gagttcatga tcagtgcaat tgtcttgtcc
     3481 ctatcgaagg actctggcac aaaatcgtat tcattaaaac cgggaggtag atgagatgtg
     3541 acgaacgtgt acatcgactg aaatccctgg taatccgttt tagaatccat gataataatt
     3601 ttttggatga ttgggagctt tttttgcacg ttcaaaattt tttgcaaccc ctttttggaa
     3661 acgaacacca cggtaggctg cgaaatgccc atactgttga gcaattcacg ttcattataa
     3721 atgtcgttcg cgggcgcaac tgcaactccg ataaataacg cgcccaacac cggcataaag
     3781 aattgaagag agttttcact gcatacgacg attctgtgat ttgtattcag cccatatcgt
     3841 ttcatagctt ctgccaaccg aacggacatt tcgaagtact cagcgtaagt gatgtccacc
     3901 tcgatatgtg catctgtaaa agcaattgtt ccaggaacca gggcgtatct cttcatagcc
     3961 ttatgcagtt gctctccagc ggttccatct tccagcggat agaatggcgc cgggcctttc
     4021 tttatgtttt tggcgtcttc catggttgtc gaccgcggag gctggatcgg tcccggtgtc
     4081 ttctatggag gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac
     4141 gagctctgct tatataggcc tcccaccgta cacgcctaca agcttctttc acttttctct
     4201 atcactgata gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg
     4261 taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt
     4321 ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg
     4381 gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta aactcgactt
     4441 tcacttttct ctatcactga tagggagtgg taaactcgag ggtaggcgtg tacggtggga
     4501 ggcctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg
     4561 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggaa ttcggccggc
     4621 cccatggaac aaaaactcat ctcagaagag gatctcgact tcaagaacct gatctggctg
     4681 aaggaggtgg acagcaccca ggagagactg aaggagtgga acgtgagcta cggcaccgcc
     4741 ctggtggccg acagacagac caagggcaga ggcggcctgg gcagaaagtg gctgagccag
     4801 gagggcggcc tgtacttcag cttcctgctg aaccccaagg agttcgagaa cctgctgcag
     4861 ctgcccctgg tgctgggcct gagcgtgagc gaggccctgg aggagatcac cgagatcccc
     4921 ttcagcctga agtggcccaa cgacgtgtac ttccaggaga agaaggtgag cggcgtgctg
     4981 tgcgagctga gcaaggacaa gctgatcgtg ggcatcggca tcaacgtgaa ccagagagag
     5041 atccccgagg agatcaagga cagagccacc accctgtacg agatcaccgg caaggactgg
     5101 gacagaaagg aggtgctgct gaaggtgctg aagagaatca gcgagaacct gaagaagttc
     5161 aaggagaagg ggcggccgcc actgtgctgg atatctgcag aattccacca cactggacta
     5221 gtggatccga gctcggtacc aagcttaagt ttaaaccgct gaagatctgg ccgcgaagtt
     5281 cctattccga agttcctatt ctctagaaag tataggaact tcagatccgg attagtccaa
     5341 tttgttaaag acaggatatc agtggtccag gctctagttt tgactcaaca atatcaccag
     5401 ctgaagccta tagagtacga gccatagata aaataaaaga ttttatttag tctccagaaa
     5461 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagctcgact tctgttcgcg
     5521 cgcttctgct ccccgagctc gagcccacaa cccctcactc ggggcgccag tcctccgatt
     5581 gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg
     5641 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt
     5701 ctttcacatg cagcatgtat caaaattaat ttggtttttt ttcttaagta tttacattaa
     5761 atggccatag tacttaaagt tacattggct tccttgaaat aaacatggag tattcagaat
     5821 gtgtcataaa tatttctaat tttaagatag tatctccatt ggctttctac tttttctttt
     5881 attttttttt gtcctctgtc ttccatttgt tgttgttgtt gtttgtttgt ttgtttgttg
     5941 gttggttggt taattttttt ttaaagatcc tacactatag ttcaagctag actattagct
     6001 actctgtaac ccagggtgac cttgaagtca tgggtagcct gctgttttag ccttcccaca
     6061 tctaagatta caggtatgag ctatcatttt tggtatattg attgattgat tgattgatgt
     6121 gtgtgtgtgt gattgtgttt gtgtgtgtga ttgtgtatat gtgtgtatgg ttgtgtgtga
     6181 ttgtgtgtat gtatgtttgt gtgtgattgt gtgtgtgtga ttgtgcatgt gtgtgtgtgt
     6241 gattgtgttt atgtgtatga ttgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     6301 gtgtgtgttg tgtatatata tttatggtag tgagaggcaa cgctccggct caggtgtcag
     6361 gttggttttt gagacagagt ctttcactta gcttggaatt aattcactgg ccgtcgtttt
     6421 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     6481 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     6541 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     6601 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     6661 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     6721 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     6781 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     6841 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     6901 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     6961 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7021 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7081 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7141 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7201 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7261 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7321 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7381 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7441 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7501 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7561 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7621 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7681 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7741 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7801 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     7861 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     7921 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     7981 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8041 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8101 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8161 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8221 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8281 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8341 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8401 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8461 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8521 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8581 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8641 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8701 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gatmccgctc gccgcagccg
     8761 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcscccaa tacgcaaacc
     8821 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     8881 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     8941 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     9001 tcacacagga aacagctatg accatgatta cgc
//
