LOCUS       sequence-340542-       12016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  make sindbis virus of 5-HT sensor GRAB_5-HT1.0 and express in cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12016)
  AUTHORS   Wan J, Peng W, Li X, Qian T, Song K, Zeng J, Deng F, Hao S, Feng J, 
            Zhang P, Zhang Y, Zou J, Pan S, Shin M, Venton BJ, Zhu JJ, Jing M, 
            Xu M, Li Y
  TITLE     A genetically encoded sensor for measuring serotonin dynamics.
  JOURNAL   Nat Neurosci. 2021 Apr 5. pii: 10.1038/s41593-021-00823-7. doi: 
            10.1038/s41593-021-00823-7.
  PUBMED    33821000
REFERENCE   2  (bases 1 to 12016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2021 Apr 5. pii: 10.1038/s41593-021-00823-7. doi: 
            10.1038/s41593-021-00823-7."
COMMENT     Sequence Label: pSin-5HT-1.0
FEATURES             Location/Qualifiers
     source          1..12016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     sig_peptide     7659..7718
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    7722..7746
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     8711..8732
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8844..8865)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9105..9124)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        10187..10291
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10292..10360,10361..11152)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10510..10529)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11323..11911
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11812..11831
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(11999..12016,1)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     11999..12016
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 attgacggcg tagtacacac tattgaatca aacagccgac caattgcact accatcacaa
       61 tggagaagcc agtagtaaac gtagacgtag acccccagag tccgtttgtc gtgcaactgc
      121 aaaaaagctt cccgcaattt gaggtagtag cacagcaggt cactccaaat gaccatgcta
      181 atgccagagc attttcgcat ctggccagta aactaatcga gctggaggtt cctaccacag
      241 cgacgatctt ggacataggc agcgcaccgg ctcgtagaat gttttccgag caccagtatc
      301 attgtgtctg ccccatgcgt agtccagaag acccggaccg catgatgaaa tatgccagta
      361 aactggcgga aaaagcgtgc aagattacaa acaagaactt gcatgagaag attaaggatc
      421 tccggaccgt acttgatacg ccggatgctg aaacaccatc gctctgcttt cacaacgatg
      481 ttacctgcaa catgcgtgcc gaatattccg tcatgcagga cgtgtatatc aacgctcccg
      541 gaactatcta tcatcaggct atgaaaggcg tgcggaccct gtactggatt ggcttcgaca
      601 ccacccagtt catgttctcg gctatggcag gttcgtaccc tgcgtacaac accaactggg
      661 ccgacgagaa agtccttgaa gcgcgtaaca tcggactttg cagcacaaag ctgagtgaag
      721 gtaggacagg aaaattgtcg ataatgagga agaaggagtt gaagcccggg tcgcgggttt
      781 atttctccgt aggatcgaca ctttatccag aacacagagc cagcttgcag agctggcatc
      841 ttccatcggt gttccacttg aatggaaagc agtcgtacac ttgccgctgt gatacagtgg
      901 tgagttgcga aggctacgta gtgaagaaaa tcaccatcag tcccgggatc acgggagaaa
      961 ccgtgggata cgcggttaca cacaatagcg agggcttctt gctatgcaaa gttactgaca
     1021 cagtaaaagg agaacgggta tcgttccctg tgtgcacgta catcccggcc accatatgcg
     1081 atcagatgac tggtataatg gccacggata tatcacctga cgatgcacaa aaacttctgg
     1141 ttgggctcaa ccagcgaatt gtcattaacg gtaggactaa caggaacacc aacaccatgc
     1201 aaaattacct tctgccgatc atagcacaag ggttcagcaa atgggctaag gagcgcaagg
     1261 atgatcttga taacgagaaa atgctgggta ctagagaacg caagcttacg tatggctgct
     1321 tgtgggcgtt tcgcactaag aaagtacatt cgttttatcg cccacctgga acgcagacct
     1381 gcgtaaaagt cccagcctct tttagcgctt ttcccatgtc gtccgtatgg acgacctctt
     1441 tgcccatgtc gctgaggcag aaattgaaac tggcattgca accaaagaag gaggaaaaac
     1501 tgctgcaggt ctcggaggaa ttagtcatgg aggccaaggc tgcttttgag gatgctcagg
     1561 aggaagccag agcggagaag ctccgagaag cacttccacc attagtggca gacaaaggca
     1621 tcgaggcagc cgcagaagtt gtctgcgaag tggaggggct ccaggcggac atcggagcag
     1681 cattagttga aaccccgcgc ggtcacgtaa ggataatacc tcaagcaaat gaccgtatga
     1741 tcggacagta tatcgttgtc tcgccaaact ctgtgctgaa gaatgccaaa ctcgcaccag
     1801 cgcacccgct agcagatcag gttaagatca taacacactc cggaagatca ggaaggtacg
     1861 cggtcgaacc atacgacgct aaagtactga tgccagcagg aggtgccgta ccatggccag
     1921 aattcctagc actgagtgag agcgccacgt tagtgtacaa cgaaagagag tttgtgaacc
     1981 gcaaactata ccacattgcc atgcatggcc ccgccaagaa tacagaagag gagcagtaca
     2041 aggttacaaa ggcagagctt gcagaaacag agtacgtgtt tgacgtggac aagaagcgtt
     2101 gcgttaagaa ggaagaagcc tcaggtctgg tcctctcggg agaactgacc aaccctccct
     2161 atcatgagct agctctggag ggactgaaga cccgacctgc ggtcccgtac aaggtcgaaa
     2221 caataggagt gataggcaca ccggggtcgg gcaagtcagc tattatcaag tcaactgtca
     2281 cggcacgaga tcttgttacc agcggaaaga aagaaaattg tcgcgaaatt gaggccgacg
     2341 tgctaagact gaggggtatg cagattacgt cgaagacagt agattcggtt atgctcaacg
     2401 gatgccacaa agccgtagaa gtgctgtacg ttgacgaagc gttcgcgtgc cacgcaggag
     2461 cactacttgc cttgattgct atcgtcaggc cccgcaagaa ggtagtacta tgcggagacc
     2521 ccatgcaatg cggattcttc aacatgatgc aactaaaggt acatttcaat caccctgaaa
     2581 aagacatatg caccaagaca ttctacaagt atatctcccg gcgttgcaca cagccagtta
     2641 cagctattgt atcgacactg cattacgatg gaaagatgaa aaccacgaac ccgtgcaaga
     2701 agaacattga aatcgatatt acaggggcca caaagccgaa gccaggggat atcatcctga
     2761 catgtttccg cgggtgggtt aagcaattgc aaatcgacta tcccggacat gaagtaatga
     2821 cagccgcggc ctcacaaggg ctaaccagaa aaggagtgta tgccgtccgg caaaaagtca
     2881 atgaaaaccc actgtacgcg atcacatcag agcatgtgaa cgtgttgctc acccgcactg
     2941 aggacaggct agtgtggaaa accttgcagg gcgacccatg gattaagcag ctcactaaca
     3001 tacctaaagg aaactttcag gctactatag aggactggga agctgaacac aagggaataa
     3061 ttgctgcaat aaacagcccc actccccgtg ccaatccgtt cagctgcaag accaacgttt
     3121 gctgggcgaa agcattggaa ccgatactag ccacggccgg tatcgtactt accggttgcc
     3181 agtggagcga actgttccca cagtttgcgg atgacaaacc acattcggcc atttacgcct
     3241 tagacgtaat ttgcattaag tttttcggca tggacttgac aagcggactg ttttctaaac
     3301 agagcatccc actaacgtac catcccgccg attcagcgag gccggtagct cattgggaca
     3361 acagcccagg aacccgcaag tatgggtacg atcacgccat tgccgccgaa ctctcccgta
     3421 gatttccggt gttccagcta gctgggaagg gcacacaact tgatttgcag acggggagaa
     3481 ccagagttat ctctgcacag cataacctgg tcccggtgaa ccgcaatctt cctcacgcct
     3541 tagtccccga gtacaaggag aagcaacccg gcccggtcga aaaattcttg aaccagttca
     3601 aacaccactc agtacttgtg gtatcagagg aaaaaattga agctccccgt aagagaatcg
     3661 aatggatcgc cccgattggc atagccggtg cagataagaa ctacaacctg gctttcgggt
     3721 ttccgccgca ggcacggtac gacctggtgt tcatcaacat tggaactaaa tacagaaacc
     3781 accactttca gcagtgcgaa gaccatgcgg cgaccttaaa aaccctttcg cgttcggccc
     3841 tgaattgcct taacccagga ggcaccctcg tggtgaagtc ctatggctac gccgaccgca
     3901 acagtgagga cgtagtcacc gctcttgcca gaaagtttgt cagggtgtct gcagcgagac
     3961 cagattgtgt ctcaagcaat acagaaatgt acctgatttt ccgacaacta gacaacagcc
     4021 gtacacggca attcaccccg caccatctga attgcgtgat ttcgtccgtg tatgagggta
     4081 caagagatgg agttggagcc gcgccgtcat accgcaccaa aagggagaat attgctgact
     4141 gtcaagagga agcagttgtc aacgcagcca atccgctggg tagaccaggc gaaggagtct
     4201 gccgtgccat ctataaacgt tggccgacca gttttaccga ttcagccacg gagacaggca
     4261 ccgcaagaat gactgtgtgc ctaggaaaga aagtgatcca cgcggtcggc cctgatttcc
     4321 ggaagcaccc agaagcagaa gccttgaaat tgctacaaaa cgcctaccat gcagtggcag
     4381 acttagtaaa tgaacataac atcaagtctg tcgccattcc actgctatct acaggcattt
     4441 acgcagccgg aaaagaccgc cttgaagtat cacttaactg cttgacaacc gcgctagaca
     4501 gaactgacgc ggacgtaacc atctattgcc tggataagaa gtggaaggaa agaatcgacg
     4561 cggcactcca acttaaggag tctgtaacag agctgaagga tgaagatatg gagatcgacg
     4621 atgagttagt atggattcat ccagacagtt gcttgaaggg aagaaaggga ttcagtacta
     4681 caaaaggaaa attgtattcg tacttcgaag gcaccaaatt ccatcaagca gcaaaagaca
     4741 tggcggagat aaaggtcctg ttccctaatg accaggaaag taatgaacaa ctgtgtgcct
     4801 acatattggg tgagaccatg gaagcaatcc gcgaaaagtg cccggtcgac cataacccgt
     4861 cgtctagccc gcccaaaacg ttgccgtgcc tttgcatgta tgccatgacg ccagaaaggg
     4921 tccacagact tagaagcaat aacgtcaaag aagttacagt atgctcctcc accccccttc
     4981 ctaagcacaa aattaagaat gttcagaagg ttcagtgcac gaaagtagtc ctgtttaatc
     5041 cgcacactcc cgcattcgtt cccgcccgta agtacataga agtgccagaa cagcctaccg
     5101 ctcctcctgc acaggccgag gaggcccccg aagttgtagc gacaccgtca ccatctacag
     5161 ctgataacac ctcgcttgat gtcacagaca tctcactgga tatggatgac agtagcgaag
     5221 gctcactttt ttcgagcttt agcggatcgg acaactctat tactagtatg gacagttggt
     5281 cgtcaggacc tagttcacta gagatagtag accgaaggca ggtggtggtg gctgacgttc
     5341 atgccgtcca agagcctgcc cctattccac cgccaaggct aaagaagatg gcccgcctgg
     5401 cagcggcaag aaaagagccc actccaccgg caagcaatag ctctgagtcc ctccacctct
     5461 cttttggtgg ggtatccatg tccctcggat caattttcga cggagagacg gcccgccagg
     5521 cagcggtaca acccctggca acaggcccca cggatgtgcc tatgtctttc ggatcgtttt
     5581 ccgacggaga gattgatgag ctgagccgca gagtaactga gtccgaaccc gtcctgtttg
     5641 gatcatttga accgggcgaa gtgaactcaa ttatatcgtc ccgatcagcc gtatcttttc
     5701 ctctacgcaa gcagagacgt agacgcagga gcaggaggac tgaatactga ctaaccgggg
     5761 taggtgggta catattttcg acggacacag gccctgggca cttgcaaaag aagtccgttc
     5821 tgcagaacca gcttacagaa ccgaccttgg agcgcaatgt cctggaaaga attcatgccc
     5881 cggtgctcga cacgtcgaaa gaggaacaac tcaaactcag gtaccagatg atgcccaccg
     5941 aagccaacaa aagtaggtac cagtctcgta aagtagaaaa tcagaaagcc ataaccactg
     6001 agcgactact gtcaggacta cgactgtata actctgccac agatcagcca gaatgctata
     6061 agatcaccta tccgaaacca ttgtactcca gtagcgtacc ggcgaactac tccgatccac
     6121 agttcgctgt agctgtctgt aacaactatc tgcatgagaa ctatccgaca gtagcatctt
     6181 atcagattac tgacgagtac gatgcttact tggatatggt agacgggaca gtcgcctgcc
     6241 tggatactgc aaccttctgc cccgctaagc ttagaagtta cccgaaaaaa catgagtata
     6301 gagccccgaa tatccgcagt gcggttccat cagcgatgca gaacacgcta caaaatgtgc
     6361 tcattgccgc aactaaaaga aattgcaacg tcacgcagat gcgtgaactg ccaacactgg
     6421 actcagcgac attcaatgtc gaatgctttc gaaaatatgc atgtaatgac gagtattggg
     6481 aggagttcgc tcggaagcca attaggatta ccactgagtt tgtcaccgca tatgtagcta
     6541 gactgaaagg ccctaaggcc gccgcactat ttgcaaagac gtataatttg gtcccattgc
     6601 aagaagtgcc tatggataga ttcgtcatgg acatgaaaag agacgtgaaa gttacaccag
     6661 gcacgaaaca cacagaagaa agaccgaaag tacaagtgat acaagccgca gaacccctgg
     6721 cgactgctta cttatgcggg attcaccggg aattagtgcg taggcttacg gccgtcttgc
     6781 ttccaaacat tcacacgctt tttgacatgt cggcggagga ttttgatgca atcatagcag
     6841 aacacttcaa gcaaggcgac ccggtactgg agacggatat cgcatcattc gacaaaagcc
     6901 aagacgacgc tatggcgtta accggtctga tgatcttgga ggacctgggt gtggatcaac
     6961 cactactcga cttgatcgag tgcgcctttg gagaaatatc atccacccat ctacctacgg
     7021 gtactcgttt taaattcggg gcgatgatga aatccggaat gttcctcaca ctttttgtca
     7081 acacagtttt gaatgtcgtt atcgccagca gagtactaga agagcggctt aaaacgtcca
     7141 gatgtgcagc gttcattggc gacgacaaca tcatacatgg agtagtatct gacaaagaaa
     7201 tggctgagag gtgcgccacc tggctcaaca tggaggttaa gatcatcgac gcagtcatcg
     7261 gtgagagacc accttacttc tgcggcggat ttatcttgca agattcggtt acttccacag
     7321 cgtgccgcgt ggcggatccc ctgaaaaggc tgtttaagtt gggtaaaccg ctcccagccg
     7381 acgacgagca agacgaagac agaagacgcg ctctgctaga tgaaacaaag gcgtggttta
     7441 gagtaggtat aacaggcact ttagcagtgg ccgtgacgac ccggtatgag gtagacaata
     7501 ttacacctgt cctactggca ttgagaactt ttgcccagag caaaagagca ttccaagcca
     7561 tcagagggga aataaagcat ctctacggtg gtcctaaata gtcagcatag tacatttcat
     7621 ctgactaata ccacaacacc accacctcta gatccaccat ggagacagac acactcctgc
     7681 tatgggtact gctgctctgg gttccaggtt ccactggtga cacaagtttg tacaaaaaag
     7741 ttggcaccac cggtgtgaac ctgaggaatg cggtgcattc attccttgtg cacctaattg
     7801 gcctattggt ttggcaatgt gatatttctg tgagcccagt agcagctata gtaactgaca
     7861 ttttcaatac ctccgatggt ggacgcttca aattcccaga cggggtacaa aactggccag
     7921 cactttcaat cgtcatcata ataatcatga caataggtgg caacatcctt gtgatcatgg
     7981 cagtaagcat ggaaaagaaa ctgcacaatg ccaccaatta cttcttaatg tccctagcca
     8041 ttgctgatat gctagtggga ctacttgtca tgcccctgtc tctcctggca atcctttatg
     8101 attatgtctg gccactacct agatatttgt gccccgtctg gatttcttta gatgttttat
     8161 tttcaacagc gtccatcatg cacctctgcg ctatatcgct ggatcggtat gtagcaatac
     8221 gtaatcctat tgagcatagc cgtttcaatt cgcggactaa ggccatcatg aagattgcta
     8281 ttgtttgggc aatttctata ggtgtatcag ttcctatccc tgtgattgga ctgagggacg
     8341 aagaaaaggt gttcgtgaac aacacgacgt gcgtgctcaa cgacccaaat ttcgttctta
     8401 ttgggtcctt cgtagctttc ttcataccgc tgacgattat ggtgattacg tattgcctga
     8461 ccatctacgt tctgcgccga caagctttga tgtttctgaa cggaaacgtc tatatcaagg
     8521 ccgacaagca gaagaacggc atcaaggcga acttccacat ccgccacaac atcgaggacg
     8581 gcggcgtgca gctcgcctac cactaccagc agaacacccc catcggcgac ggccccgtgc
     8641 tgctgcccga caaccactac ctgagcgtgc agtccaaact ttcgaaagac cccaacgaga
     8701 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cggaatcact ctcggcatgg
     8761 acgagctgta caagggcggt accggagggc aagaatccat gagcagcaag ggcgaggagc
     8821 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     8881 tcagcgtgtc cggcgagggt gagggcgatg ccaccactgg caagctgacc ctgaagttca
     8941 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     9001 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     9061 ccatgcccga aggctacatc caggagcgca ccatcttctt caaggacgac ggcaactaca
     9121 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     9181 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aacggatttg
     9241 caacagcaaa caatgaaaga aaagcttcga aagtccttgg gattgttttc tttgtgtttc
     9301 tgatcatgtg gtgcccattt ttcattacca atattctgtc tgttctttgt gagaagtcct
     9361 gtaaccaaaa gctcatggaa aagcttctga atgtgtttgt ttggattggc tatgtttgtt
     9421 caggaatcaa tcctctggtg tatactctgt tcaacaaaat ttaccgaagg gcattctcca
     9481 actatttgcg ttgcaattat aaggtagaga aaaagcctcc tgtcaggcag attccaagag
     9541 ttgccgccac tgctttgtct gggagggagc ttaatgttaa catttatcgg cataccaatg
     9601 aaccggtgat cgagaaagcc agtgacaatg agcccggtat agagatgcaa gttgagaatt
     9661 tagagttacc agtaaatccc tccagtgtgg ttagcgaaag gattagcagt gtgtgagcat
     9721 gcaggccttg ggcccaatga tccgaccagc aaaactcgat gtacttccga ggaactgatg
     9781 tgcataatgc atcaggctgg tacattagat ccccgcttac cgcgggcaat atagcaacac
     9841 taaaaactcg atgtacttcc gaggaagcgc agtgcataat gctgcgcagt gttgccacat
     9901 aaccactata ttaaccattt atctagcgga cgccaaaaac tcaatgtatt tctgaggaag
     9961 cgtggtgcat aatgccacgc agcgtctgca taacttttat tatttctttt attaatcaac
    10021 aaaattttgt ttttaacatt tcaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaagg
    10081 ggaattcctc gataattaag cggccgctcg agcggccgct taattaatcg aggggaatta
    10141 attcttgaag acgaaagggc caggtggcac ttttcgggga aatgtgcgcg gaacccctat
    10201 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
    10261 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
    10321 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
    10381 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
    10441 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
    10501 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
    10561 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
    10621 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
    10681 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
    10741 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
    10801 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
    10861 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
    10921 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
    10981 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
    11041 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
    11101 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
    11161 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
    11221 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
    11281 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
    11341 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
    11401 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
    11461 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
    11521 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
    11581 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
    11641 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    11701 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    11761 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    11821 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    11881 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcgagct cgtatggaca
    11941 tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat
    12001 ttaggtgaca ctatag
//
