LOCUS       sequence-340806-        4402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pQE30 encoding Wzz mutant  i138.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Papadopoulos M, Morona R
  TITLE     Mutagenesis and chemical cross-linking suggest that Wzz dimer 
            stability and oligomerization affect lipopolysaccharide O-antigen 
            modal chain length control.
  JOURNAL   J Bacteriol. 2010 Jul;192(13):3385-93. doi: 10.1128/JB.01134-09. 
            Epub 2010 May 7.
  PUBMED    20453100
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.01134-09"; journalName: "J Bacteriol"; date: "2010-07"; 
            volume: "192"; issue: "13"; pages: "3385-93"
COMMENT     Sequence Label: pMPRMA52
FEATURES             Location/Qualifiers
     source          1..4402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     177..196
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     RBS             497..508
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             527..544
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1768..1862
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1906..2565
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1972..1991)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2630..2716
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2763..2785)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2871..3011
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2926..2945
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3026..3043)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3197..3785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3277..3296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3956..4402,1..345,346..414))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
       61 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
      121 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
      181 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
      241 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
      301 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
      361 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
      421 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gataacaatt
      481 tcacacagaa ttcattaaag aggagaaatt aactatgaga ggatcgcatc accatcacca
      541 tcacggatcc gcatgcgagc tcagagtaga aaataataat gtttctgggc aaaaccatga
      601 cccggaacag attgatttga ttgatttact agtgcagttg tggcgtggca agatgacaat
      661 tatcatttcc gtcattgtgg ctattgccct ggctattggt tatttggcag tagcgaagga
      721 gaaatggacg tcaacagcaa ttatcactca gcccgacgtg gggcaaattg ctggctataa
      781 caatgccatg aatgttatct atggtcaggc tgcaccgaaa gtatcggatt tgcaggagac
      841 gttaattggt cgcttcagtt ctgccttctc tgcattagca gaaacgctgg ataatcagga
      901 agagccagaa aaacttacca tcgaaccttc tgttaagaac cagcaattac cattgactgt
      961 ttcttatgtt gggtgcggcc gcattgggca aactgcagag ggcgcacaaa tgaagttggc
     1021 ccaatacatt cagcaagttg atgataaagt gaatcaagag ctagaaaagg atctcaagga
     1081 caacattgct ctgggacgga aaaacttgca ggactcttta agaacccagg aagtggtcgc
     1141 gcaggagcag aaagatctgc gtatccgtca gattcaggaa gcgttgcagt atgcgaatca
     1201 ggcgcaggtg acaaagccac aggttcagca gactgaagat gtgacgcaag atacgttgtt
     1261 ccttctaggg agcgaagcgc tggagtcgat gattaagcat gaagcgactc gtccgttggt
     1321 gttctcacca aactactatc agacacgtca aaacctgttg gatattgaaa aattaaagtt
     1381 tgatgatctt gatattcatg cttaccgcta tgtgatgaaa ccgacgttac ctattcgtcg
     1441 cgatagtccg aaaaaggcaa tcaccttgat tctggcagtg cttctgggcg gcatggttgg
     1501 cgcggggatt gtgttggggc gtaacgctct gcgtaattac aacgcgaagt aatattattg
     1561 tgcatttaag agaaacgggc agggtggtga caccatgccc gttttttttg ccggatgcga
     1621 tgctggcgca tcttatccgg cctacgtgtg ttgagataat gtgtaggcac gataagtttg
     1681 cgcatcgggc aatggctccg ggtgtgacaa caacatcaca cctgctcccc gggtcgacct
     1741 gcagccaagc ttaattagct gagcttggac tcctgttgat agatccagta atgacctcag
     1801 aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga
     1861 atccaagcta gcttggcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     1921 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     1981 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     2041 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     2101 ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg
     2161 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     2221 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     2281 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     2341 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     2401 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     2461 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     2521 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     2581 tattggtgcc cttaaacgcc tggggtaatg actctctagc ttgaggcatc aaataaaacg
     2641 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     2701 cctgagtagg acaaatccgc cctctagagc tgcctcgcgc gtttcggtga tgacggtgaa
     2761 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     2821 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     2881 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     2941 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     3001 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3061 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3121 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3181 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3241 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3301 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3361 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3421 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3481 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3541 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3601 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     3661 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3721 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3781 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3841 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3901 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3961 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4021 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4081 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4141 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4201 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4261 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4321 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4381 gctccttcgg tcctccgatc gt
//
