LOCUS       sequence-341138-        9228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mCherry-PHR-iSH-T2A-CIBNcaax (opto-PI3K).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9228)
  AUTHORS   Kakumoto T, Nakata T
  TITLE     Optogenetic control of PIP3: PIP3 is sufficient to induce the 
            actin-based active part of growth cones and is regulated via 
            endocytosis.
  JOURNAL   PLoS One. 2013 Aug 7;8(8):e70861. doi: 10.1371/journal.pone.0070861.
            eCollection 2013.
  PUBMED    23951027
REFERENCE   2  (bases 1 to 9228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-08-7"; volume: "8(8):e70861. doi"; pages: " 
            10.1371/journal.pone.0070861. eCollection 2013"
COMMENT     Sequence Label: mCherry-PHR-iSH-T2A-CIBNcaax
FEATURES             Location/Qualifiers
     source          1..9228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          148..1164
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1088..1110
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1172..1191
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1242..1251
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1248..1955
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1393..1411)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1661..1680
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2007..3500
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             4134..4187
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4200..4709
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     misc_feature    4801..5389
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4854..4874)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5272..5283)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5528..5649
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5565..5584)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5619..5638
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5656..6111)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5793..5814)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6005..6024
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6138..6242
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6244..6601
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6244..6435
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6267..6287)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6452..6587
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6636..7430
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6636..7430
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6690..6709)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7300..7319
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7618..7637)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7662..7709
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8038..8626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8527..8546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8801..9104
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc
       61 aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc
      121 tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc
      181 cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag
      241 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg
      301 cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc
      361 ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc
      421 tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc
      481 agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag
      541 gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg
      601 tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct
      661 tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg
      721 cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag
      781 gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc
      841 cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag
      901 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc
      961 gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt
     1021 ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc
     1081 agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt
     1141 catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat
     1201 cattttggca aagaattgat ttatcgctag cgctaccggt cgccaccatg gtgagcaagg
     1261 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     1321 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     1381 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     1441 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     1501 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     1561 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     1621 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     1681 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     1741 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     1801 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     1861 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     1921 gccactccac cggcggcatg gacgagctgt acaagtccgg actcagatct cgagctcaag
     1981 cttcgaattc tgcagtcgac ggtaccatga agatggacaa aaagactata gtttggttta
     2041 gaagagacct aaggattgag gataatcctg cattagcagc agctgctcac gaaggatctg
     2101 tttttcctgt cttcatttgg tgtcctgaag aagaaggaca gttttatcct ggaagagctt
     2161 caagatggtg gatgaaacaa tcacttgctc acttatctca atccttgaag gctcttggat
     2221 ctgacctcac tttaatcaaa acccacaaca cgatttcagc gatcttggat tgtatccgcg
     2281 ttaccggtgc tacaaaagtc gtctttaacc acctctatga tcctgtttcg ttagttcggg
     2341 accataccgt aaaggagaag ctggtggaac gtgggatctc tgtgcaaagc tacaatggag
     2401 atctattgta tgaaccgtgg gagatatact gcgaaaaggg caaacctttt acgagtttca
     2461 attcttactg gaagaaatgc ttagatatgt cgattgaatc cgttatgctt cctcctcctt
     2521 ggcggttgat gccaataact gcagcggctg aagcgatttg ggcgtgttcg attgaagaac
     2581 tagggctgga gaatgaggcc gagaaaccga gcaatgcgtt gttaactaga gcttggtctc
     2641 caggatggag caatgctgat aagttactaa atgagttcat cgagaagcag ttgatagatt
     2701 atgcaaagaa cagcaagaaa gttgttggga attctacttc actactttct ccgtatctcc
     2761 atttcgggga aataagcgtc agacacgttt tccagtgtgc ccggatgaaa caaattatat
     2821 gggcaagaga taagaacagt gaaggagaag aaagtgcaga tctttttctt aggggaatcg
     2881 gtttaagaga gtattctcgg tatatatgtt tcaacttccc gtttactcac gagcaatcgt
     2941 tgttgagtca tcttcggttt ttcccttggg atgctgatgt tgataagttc aaggcctgga
     3001 gacaaggcag gaccggttat ccgttggtgg atgccggaat gagagagctt tgggctaccg
     3061 gatggatgca taacagaata agagtgattg tttcaagctt tgctgtgaag tttcttctcc
     3121 ttccatggaa atggggaatg aagtatttct gggatacact tttggatgct gatttggaat
     3181 gtgacatcct tggctggcag tatatctctg ggagtatccc cgatggccac gagcttgatc
     3241 gcttggacaa tcccgcgtta caaggcgcca aatatgaccc agaaggtgag tacataaggc
     3301 aatggcttcc cgagcttgcg agattgccaa ctgaatggat ccatcatcca tgggacgctc
     3361 ctttaaccgt actcaaagct tctggtgtgg aactcggaac aaactatgcg aaacccattg
     3421 tagacatcga cacagctcgt gagctactag ctaaagctat ttcaagaacc cgtgaagcac
     3481 agatcatgat cggagcagca agtgctggag gtagtgctgg aggttcagct ggtggaccta
     3541 gagcatccaa gtaccaacaa gaccaggtgg tgaaggagga cagcgtagag gctgtgggcg
     3601 cccagctcaa ggtctaccac cagcagtacc aggacaagag ccgcgaatat gaccagctgt
     3661 atgaagaata cacacggacc tcccaggagc tgcagatgaa gcgcacagcc atagaggcct
     3721 tcaacgagac catcaagatc ttcgaagagc agggccagac acaggagaag tgcagcaagg
     3781 agtatttgga gcgcttccgg cgagagggaa atgagaagga gatgcagagg atcctgctga
     3841 actccgagcg actcaagtct cgcatcgcgg agatacacga aagccgcacg aagttggagc
     3901 aggatctgcg ggcgcaggcc tccgacaacc gtgagatcga caagcgcatg aacagcctca
     3961 aacctgacct catgcagctg cgcaagatca gggaccagta cctcgtgtgg ctcacccaga
     4021 aaggtgcccg acagaggaag atcaacgaat ggctgggaat caagaacgag actgaggacc
     4081 agtattcact gatggaggat gaggacgccc tcccccacca cgaggagcgc acggagggcc
     4141 gcggaagtct tctaacatgc ggggacgtgg aggaaaatcc cggccccgca ccgggatcca
     4201 tgaatggagc tataggaggt gaccttttgc tcaattttcc tgacatgtcg gtcctagagc
     4261 gccaaagggc tcacctcaag tacctcaatc ccacctttga ttctcctctc gccggcttct
     4321 ttgccgattc ttcaatgatt accggcggcg agatggacag ctatctttcg actgccggtt
     4381 tgaatcttcc gatgatgtac ggtgagacga cggtggaagg tgattcaaga ctctcaattt
     4441 cgccggaaac gacgcttggg actggaaatt tcaaggcagc gaagtttgat acagagacta
     4501 aggattgtaa tgaggcggcg aagaagatga cgatgaacag agatgaccta gtagaagaag
     4561 gagaagaaga gaagtcgaaa ataacagagc aaaacaatgg gagcacaaaa agcatcaaga
     4621 agatgaaaca caaagccaag aaagaagaga acaatttctc taatgattca tctaaagtga
     4681 cgaaggaatt ggagaaaacg gattatattc atgtactgta taagggtaaa aagaagaaaa
     4741 agaagtcaaa gacaaagtgt gtaattatgt agcggccgca ttggatctaa gcttatcgat
     4801 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4861 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4921 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4981 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     5041 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     5101 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     5161 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     5221 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     5281 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     5341 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     5401 ggatccaagg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact
     5461 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     5521 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5581 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5641 tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     5701 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     5761 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     5821 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     5881 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     5941 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     6001 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     6061 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     6121 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6181 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6241 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     6301 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     6361 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6421 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6481 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6541 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6601 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     6661 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     6721 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     6781 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
     6841 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     6901 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     6961 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     7021 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     7081 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     7141 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
     7201 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     7261 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     7321 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     7381 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     7441 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     7501 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     7561 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
     7621 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     7681 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     7741 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     7801 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     7861 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
     7921 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7981 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8041 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8101 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8161 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8221 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8281 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8341 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8401 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8461 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8521 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8581 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8641 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8701 atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa
     8761 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     8821 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     8881 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     8941 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     9001 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     9061 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     9121 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     9181 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggg
//
