LOCUS       sequence-341322-         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl 
            transferase. R4-4 can use 3′-dephospho CoA for adding the 
            phosphopantetheinyl group to the carrier proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Sunbul M, Marshall NJ, Zou Y, Zhang K, Yin J
  TITLE     Catalytic turnover-based phage selection for engineering the 
            substrate specificity of Sfp phosphopantetheinyl transferase.
  JOURNAL   J Mol Biol. 2009 Apr 10;387(4):883-98. doi: 
            10.1016/j.jmb.2009.02.010.
  PUBMED    19340948
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2009.02"; journalName: "J Mol Biol"; date: 
            "2009-04-10- 10"; volume: "387"; issue: "4"; pages: "883-98"
COMMENT     Sequence Label: pET-Sfp R4-4 mutant
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             679..696
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgaagattt acggaattta tatggaccgc ccgctttcac aggaagaaaa tgaacggttc
       61 atgactttca tatcacctga agagcgggag aagtgtcgac gattttatca taaagaagat
      121 gctcaccgcg agctcctagg agatgtgctc gttcgctcag tcataagcag gcagtatcag
      181 ttggacaaat ccgatatccg ctttagcacg caggaatacg ggaagccgta catccctgat
      241 cttcccgacg ctcatttcaa catttctcat tccggccgct gggtcattgg tgcgtttgat
      301 tcacagccga tcggcataga tatcgaaaaa acgaaaccga tcagccttga gatcgccaag
      361 cgcttctttt caaaaacaga gtacagcgac cttttagcaa aagacaagga cgagcagaca
      421 gactattttt atcatctatg gtcaatgaaa gaaagcttta tcaaacagga aggcaaaggc
      481 ttatcgcttc cgcttgattc cttttcagtg cgcctgcatc aggacggaca agtatccatt
      541 gagcttccgg acagccattc cccatgctat atcaaaacgt atgaggtcga tcccggctac
      601 aaaatggctg tatgcgccgc acaccctgat ttccccgagg atatcacaat ggtctcgtac
      661 gaagagcttt tactcgagca ccaccaccac caccac
//
