LOCUS       sequence-341512-       14525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UCOE-SFFV-Cas9-BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14525)
  AUTHORS   Hein MY, Weissman JS
  TITLE     Functional single-cell genomics of human cytomegalovirus infection.
  JOURNAL   Nat Biotechnol. 2022 Mar;40(3):391-401. doi: 
            10.1038/s41587-021-01059-3. Epub 2021 Oct 25.
  PUBMED    34697476
REFERENCE   2  (bases 1 to 14525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-021-01059-3"; journalName: "Nat Biotechnol"; date: 
            "2022-03"; volume: "40"; issue: "3"; pages: "391-401"
COMMENT     Sequence Label: pMH0004
FEATURES             Location/Qualifiers
     source          1..14525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      36..171
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1319..1384
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1514..1534
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1959..2093
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1996..2015)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2050..2069
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2261..2894
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2941..3066
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3563..3796
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3981..4025
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4310..4427
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5169..5576
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      5645..5654
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5651..9754
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5651..9754
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9758..9784
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             9764..9790
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             9803..9823
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9830..9850
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9890..10588
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     misc_feature    10649..11237
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10702..10722)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11120..11131)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11240..11256)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11241..11257)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11347..11580
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(11605..11623)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(11606..11623)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(11913..11932)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(12146..12165)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12265..12287
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12325..12343)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12411..12515
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12516..12584,12585..13376)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12734..12753)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13547..14135
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14036..14055
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14289..14306
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(14376..14396)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(14381..14525,1..185)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
       61 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
      121 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
      181 gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc
      241 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
      301 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
      361 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
      421 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
      481 attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc
      541 gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag
      601 tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata
      661 ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct
      721 tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata
      781 cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct
      841 aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa
      901 tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc
      961 ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac
     1021 ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc
     1081 atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc
     1141 caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg
     1201 attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc
     1261 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
     1321 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
     1381 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
     1441 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
     1501 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
     1561 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
     1621 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
     1681 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
     1741 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
     1801 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
     1861 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     1921 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     1981 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     2041 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
     2101 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
     2161 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
     2221 gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc
     2281 caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     2341 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
     2401 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
     2461 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
     2521 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
     2581 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
     2641 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
     2701 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     2761 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     2821 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     2881 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     2941 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     3001 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     3061 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     3121 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     3181 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     3241 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     3301 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     3361 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct
     3421 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     3481 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     3541 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     3601 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     3661 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     3721 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     3781 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     3841 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     3901 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     3961 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     4021 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     4081 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     4141 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     4201 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     4261 tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt catccacaat tttaaaagaa
     4321 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     4381 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     4441 gggacagcag agatccagtt tggatcgata agcttgatat cgaattcggg aggtggtccc
     4501 tgcagttacg ccaatgataa cccccgccag aaaaatctta gtagccttcc ctttttgttt
     4561 tccgtgcccc aactcggcgg attgactcgg ccccttccgg aaacacccga atcaacttct
     4621 agtcaaatta ttgttcacgc cgcaatgacc cacccctggc ccgcgtctgt ggaactgacc
     4681 cctggtgtac aggagagttc gctgctgaaa gtggtcccaa aggggtacta gtttttaagc
     4741 tcccaactcc ccctccccca gcgtctggag gattccacac cctcgcaccg caggggcgag
     4801 gaagtgggcg gagtccggtt ttggcgccag ccgctgaggc tgccaagcag aaaagccacc
     4861 gctgaggaga ctccggtcac tgtcctcgcc ccgcctcccc cttccctccc cttggggacc
     4921 accgggcgcc acgccgcgaa cggtaagtgc cgcggtcgtc ggcgcctccg ccctccccct
     4981 agggccccaa ttcccagcgg gcgcggcgcg cggccccgcg cgcagctccc ggctccctcc
     5041 cccttcggat gtggcttgag ctgtaggcgc ggagtcctgc agccccgata aaataaaaga
     5101 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc
     5161 tagctgcagt aacgccattt tgcaaggcat ggaaaaatac caaaccaaga atagagaagt
     5221 tcagatcaag ggcgggtaca tgaaaatagc taacgttggg ccaaacagga tatctgcggt
     5281 gagcagtttc ggccccggcc cggggccaag aacagatggt caccgcagtt tcggccccgg
     5341 cccgaggcca agaacagatg gtccccagat atggcccaac cctcagcagt ttcttaagac
     5401 ccatcagatg tttccaggct cccccaagga cctgaaatga ccctgcgcct tatttgaatt
     5461 aaccaatcag cctgcttctc gcttctgttc gcgcgcttct gcttcccgag ctctataaaa
     5521 gagctcacaa cccctcactc ggcgcgccag tcctccgaca gactgagtcg cccggggggg
     5581 atctggagct ctcgagaatt ctcacgcgtc tgcaggatat caagcttgcg gtaccgcggg
     5641 cccggccacc atggacaaga agtacagcat cggcctggac atcggcacca actctgtggg
     5701 ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa
     5761 caccgaccgg cacagcatca agaagaacct gatcggcgcc ctgctgttcg acagcggaga
     5821 aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa
     5881 ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt
     5941 cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc
     6001 catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca
     6061 cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc
     6121 cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga
     6181 caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga
     6241 ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag
     6301 caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct
     6361 gttcggcaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga
     6421 cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa
     6481 cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc
     6541 cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct
     6601 gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc
     6661 tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa
     6721 cggctacgcc ggctacatcg atggcggagc cagccaggaa gagttctaca agttcatcaa
     6781 gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga
     6841 cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg
     6901 agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg
     6961 ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag
     7021 gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa
     7081 cttcgaggaa gtggtggaca agggcgccag cgcccagagc ttcatcgagc ggatgaccaa
     7141 cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta
     7201 cttcaccgtg tacaacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc
     7261 cgccttcctg agcggcgagc agaaaaaagc catcgtggac ctgctgttca agaccaaccg
     7321 gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc
     7381 cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct
     7441 gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga
     7501 agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa
     7561 aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac
     7621 cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa
     7681 gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat
     7741 ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg
     7801 cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat
     7861 cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga
     7921 gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag
     7981 ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa
     8041 agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca
     8101 gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga
     8161 tgtggaccat atcgtgcctc agagctttct gaaggacgac tccatcgata acaaagtgct
     8221 gactcggagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa
     8281 gaagatgaag aactactggc gccagctgct gaatgccaag ctgattaccc agaggaagtt
     8341 cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat
     8401 caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc
     8461 ccggatgaac actaagtacg acgagaacga caaactgatc cgggaagtga aagtgatcac
     8521 cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga
     8581 gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct
     8641 gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga
     8701 cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt
     8761 cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat
     8821 ccggaagcgg cctctgatcg agacaaacgg cgaaacaggc gagatcgtgt gggataaggg
     8881 ccgggacttt gccaccgtgc ggaaagtgct gtctatgccc caagtgaata tcgtgaaaaa
     8941 gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga
     9001 caagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc
     9061 caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact
     9121 gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa
     9181 tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa
     9241 gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc
     9301 cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta
     9361 cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct
     9421 gtttgtggaa cagcacaaac actacctgga cgagatcatc gagcagatca gcgagttctc
     9481 caagagagtg atcctggccg acgctaatct ggacaaggtg ctgagcgcct acaacaagca
     9541 cagagacaag cctatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa
     9601 tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac
     9661 cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga
     9721 gacacggatc gacctgtctc agctgggagg cgacgcctat ccctatgacg tgcccgatta
     9781 tgccagcctg ggcagcggct cccccaagaa aaaacgcaag gtggaagatc ctaagaaaaa
     9841 gcggaaagtg gacggcattg gtagtgggag caacggcagc agcggatcca gcgagctgat
     9901 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa
     9961 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
    10021 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg
    10081 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc
    10141 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
    10201 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
    10261 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga
    10321 gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt
    10381 gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa
    10441 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc
    10501 caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc
    10561 tagcaaactg gggcacaagc ttaattagta aggccgcgac tctagagtcg acctgcaggc
    10621 atgcaagctt gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
    10681 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
    10741 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
    10801 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
    10861 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
    10921 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
    10981 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
    11041 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
    11101 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
    11161 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
    11221 ttgggccgcc tccccgcatc gataccgtcg acctcgaggg aattaattcg agctcggtac
    11281 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
    11341 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
    11401 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
    11461 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
    11521 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
    11581 gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca
    11641 taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt
    11701 agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg
    11761 tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag
    11821 cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg
    11881 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
    11941 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
    12001 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
    12061 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
    12121 agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
    12181 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
    12241 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
    12301 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
    12361 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
    12421 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
    12481 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    12541 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    12601 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
    12661 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
    12721 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
    12781 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    12841 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    12901 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    12961 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    13021 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    13081 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    13141 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    13201 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    13261 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    13321 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    13381 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    13441 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    13501 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    13561 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    13621 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    13681 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    13741 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    13801 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    13861 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    13921 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    13981 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    14041 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    14101 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    14161 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    14221 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    14281 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    14341 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg
    14401 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
    14461 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    14521 caatt
//
