LOCUS       sequence-341660-       11087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of PIP5K1B (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11087)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-PIP5K1B_IDG-K
FEATURES             Location/Qualifiers
     source          1..11087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             43..642
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(43..62)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     539..559
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             780..1013
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1085..1206
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1122..1141)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1176..1195
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1246..1381
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1401..1420)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1402..1420)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1430..1447)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1430..1446)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1439..1461)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1588..2043
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1675..1694)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1885..1906
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2069..2173
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2174..2242,2243..3034)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3205..3793
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3694..3713
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3947..3964
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4081..4102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4117..4122,4123..4140,4141..4147)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4155..4171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4160..4182
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4179..4195
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4179..4195
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4214..4234
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4216..4234
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4262..4488
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4489..4669
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4716..4841
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5334..5567
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5752..5796
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6063..6179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6213..6233
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6233..7444
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7281..7300
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7448..7468)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7469..7478
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7481..8251
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8258..8299
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8300..8324
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9954..9978)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    10008..10596
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10061..10081)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10479..10490)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(10610..11087,1..22)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10645..10668)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     11042..11061
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc
       61 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
      121 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
      181 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
      241 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
      301 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
      361 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
      421 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
      481 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
      541 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
      601 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
      661 cagcgcccga ccgaaaggag cgcacgaccc catgcatctc gagggcccgg tacctttaag
      721 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
      781 ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     1021 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     1081 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     1141 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     1201 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     1261 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     1321 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     1381 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     1441 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     1501 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     1561 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     1621 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     1681 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     1741 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     1801 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     1861 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     1921 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     1981 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     2041 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     2101 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     2161 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     2221 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     2281 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     2341 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     2401 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     2461 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     2521 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     2581 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     2641 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     2701 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     2761 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     2821 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     2881 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     2941 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     3001 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     3061 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     3121 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     3181 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     3241 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     3301 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     3361 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3421 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     3481 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3541 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     3601 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3661 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3721 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3781 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3841 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     3901 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     3961 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     4021 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     4081 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     4141 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     4201 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     4261 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     4321 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     4381 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     4441 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     4501 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     4561 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     4621 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     4681 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     4741 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     4801 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     4861 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     4921 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     4981 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     5041 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     5101 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     5161 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     5221 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     5281 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     5341 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     5401 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     5461 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     5521 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     5581 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     5641 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     5701 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     5761 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     5821 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     5881 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     5941 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     6001 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     6061 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     6121 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     6181 tcgtccagtg tggtggaatt ctgcagatat cacaactttg tatagaaaag ttggcctccg
     6241 cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca
     6301 gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc
     6361 tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac
     6421 ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag
     6481 tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat
     6541 aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc
     6601 ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg
     6661 ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct
     6721 gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa
     6781 tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg
     6841 aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg
     6901 cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa
     6961 gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg
     7021 tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc
     7081 acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc
     7141 ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc
     7201 gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg
     7261 tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag
     7321 tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa
     7381 ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt
     7441 agacggccaa cttttgtata caaagtgggc caccatggga atcccgctgc tgaacgctaa
     7501 acagattctg ggacagctgg acggcgggag cgtggcagtc ctgcctgtgg tcgactccac
     7561 caatcagtac ctgctggatc gaatcggcga gctgaagagt ggggatgctt gcattgcaga
     7621 atatcagcag gcagggagag gaagcagagg gaggaaatgg ttctctcctt ttggagctaa
     7681 cctgtacctg agtatgtttt ggcgcctgaa gcggggacca gcagcaatcg gcctgggccc
     7741 ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg ggagcagaca aggtgcgagt
     7801 caaatggccc aatgacctgt atctgcagga tagaaagctg gcaggcatcc tggtggagct
     7861 ggccggaata acaggcgatg ctgcacagat cgtcattggc gccgggatta acgtggctat
     7921 gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc acactgcagg aagcagggat
     7981 taacctggac aggaatactc tggccgctat gctgatccga gagctgcggg cagccctgga
     8041 actgttcgag caggaaggcc tggctccata tctgtcacgg tgggagaagc tggataactt
     8101 catcaataga cccgtgaagc tgatcattgg ggacaaagag attttcggga ttagccgggg
     8161 gattgataaa cagggagccc tgctgctgga acaggacgga gttatcaaac cctggatggg
     8221 cggagaaatc agtctgcggt ctgccgaaaa ggctagcggc aagcccatcc ccaaccccct
     8281 gctgggcctg gacagcacca caagtttgta caaaaaagca ggctccacca tgtcttctgc
     8341 tgctgaaaat ggagaggcag cacctggaaa acaaaatgaa gaaaaaacct ataaaaagac
     8401 tgcatcatct gctattaaag gtgccattca gctgggaata ggatacacag tgggtaatct
     8461 cacttccaag ccagaacgag atgttcttat gcaagacttt tatgtggtgg aaagtgtgtt
     8521 cctacccagc gaagggagca atctgacccc agcacatcac tacccagact ttagatttaa
     8581 gacatacgct ccattagcat tccgatattt cagagaactt tttggtatca agcctgatga
     8641 ttacttgtat tccatctgca gtgaacctct aatagaactg tctaaccctg gagccagtgg
     8701 atccttgttt tttgtgacca gtgatgatga atttatcatc aaaacagttc agcacaaaga
     8761 agctgagttt cttcagaagc tactgccagg ctattacatg aatttaaacc agaatccaag
     8821 gactcttttg ccaaaatttt acggactgta ttgtatgcaa tcaggaggca ttaatatcag
     8881 gattgtggtg atgaacaacg ttttgccacg ctccatgaga atgcacttta catatgactt
     8941 gaaaggctca acgtataagc gaagagcatc ccgtaaagag agagagaaat ccaaccccac
     9001 atttaaggac ttagatttcc tgcaagacat gcacgaaggg ttgtattttg atacggaaac
     9061 atacaacgcg cttatgaaaa cacttcagag agactgccgg gtgctagaaa gcttcaagat
     9121 catggattat agccttctgt tgggaattca tttcctggac cattccctca aagagaaaga
     9181 ggaggagacc ccacaaaatg tgcctgatgc taagcggact gggatgcaga aggttctcta
     9241 ctcaacagcc atggaatcta tccagggtcc agggaaatct ggagatggga taatcacaga
     9301 gaacccagac acaatgggag gcattccagc taaaagccat aggggagaaa aactactttt
     9361 atttatgggc attattgaca ttctgcaatc atataggtta atgaagaagt tagaacattc
     9421 ctggaaagct cttgtttatg atggggacac tgtttctgtt catagaccaa gcttttatgc
     9481 agacagattt cttaagttca tgaattccag agttttcaag aaaattcaag ctttgaaggc
     9541 ttcaccgtct aagaaacggt gcaattcaat cgccgcccta aaggccactt cacaggagat
     9601 tgtgtcctca attagccagg aatggaagga tgagaagcgg gatttgctga ctgaaggaca
     9661 aagttttagc agccttgatg aagaagccct gggatcccga cacaggccag acctggtccc
     9721 tagcactcca tcactgtttg aagctgcttc cttggcaacc acaatttcat cttcttcctt
     9781 atacgtcaat gagcactatc cacacgacag gcctacactc tattcaaaca gcaaagggtt
     9841 accttccagt tcaacattta ccttggaaga ggggaccatc tacttgaccg ctgagcccaa
     9901 cactctggaa gtgcaggatg acaatgcttc tgtgcttgac gtctatttat aggacccagc
     9961 tttcttgtac aaagtggtga tatccagcac agtggcggcc gctcgacaat caacctctgg
    10021 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
    10081 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
    10141 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
    10201 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
    10261 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
    10321 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
    10381 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
    10441 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
    10501 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
    10561 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattctaccg ggtaggggag
    10621 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
    10681 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
    10741 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
    10801 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
    10861 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
    10921 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
    10981 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
    11041 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctc
//
