LOCUS       sequence-341663-       10226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of TP53RK (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10226)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-TP53RK_IDG-K
FEATURES             Location/Qualifiers
     source          1..10226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             56..655
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(56..75)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     552..572
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             793..1026
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1098..1219
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1135..1154)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1189..1208
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1259..1394
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1414..1433)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1415..1433)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1443..1460)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1443..1459)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1452..1474)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1601..2056
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1688..1707)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1898..1919
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2082..2186
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2187..2255,2256..3047)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3218..3806
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3707..3726
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3960..3977
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4094..4115
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4130..4135,4136..4153,4154..4160)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4168..4184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4173..4195
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4192..4208
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4192..4208
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4227..4247
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4229..4247
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4275..4501
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4502..4682
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4729..4854
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5347..5580
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5765..5809
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6076..6192
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6226..6246
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6246..7457
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7294..7313
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7461..7481)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7482..7491
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7494..8264
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8271..8312
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8313..8337
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      8341..8350
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(9106..9130)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9160..9748
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9213..9233)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9631..9642)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(9762..10226,1..35)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9797..9820)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10194..10213
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac
       61 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
      121 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
      181 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
      241 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
      301 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
      361 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
      421 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
      481 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
      541 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
      601 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc
      661 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat ctcgagggcc
      721 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
      781 aaaagggggg actggaaggg ctagctcact cccaacgaag acaagatctg ctttttgctt
      841 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
      901 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
      961 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     1021 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     1081 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     1141 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     1201 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     1261 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     1321 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     1381 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     1441 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     1501 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     1561 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     1621 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     1681 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     1741 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     1801 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     1861 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     1921 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     1981 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     2041 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2101 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2161 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2221 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2281 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2341 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2401 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     2461 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2521 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     2581 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     2641 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     2701 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     2761 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     2821 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     2881 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     2941 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     3001 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     3061 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3121 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3181 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3241 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3301 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3361 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3421 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3481 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3541 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3601 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3661 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3721 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3781 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3841 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3901 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3961 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     4021 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4081 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     4141 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     4201 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     4261 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     4321 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     4381 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     4441 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     4501 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     4561 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     4621 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     4681 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     4741 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     4801 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     4861 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     4921 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     4981 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     5041 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     5101 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     5161 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     5221 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     5281 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     5341 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     5401 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     5461 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     5521 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     5581 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     5641 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     5701 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     5761 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     5821 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     5881 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     5941 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     6001 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
     6061 acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     6121 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     6181 attcaaaatt ttatcgtcca gtgtggtgga attctgcaga tatcacaact ttgtatagaa
     6241 aagttggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag
     6301 cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag
     6361 gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc agaaggacat
     6421 tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga gcggaacagg
     6481 cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg gcggtgaacg
     6541 ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc agccgggatt
     6601 tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta gcgggctgct
     6661 gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc gtgtggagag
     6721 accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg gggttggggg
     6781 gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc
     6841 tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca aggtcttgag
     6901 gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca ccatctgggg
     6961 accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt tgcgggggcg
     7021 gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc
     7081 gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac ctgccggtag
     7141 gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg taggctctcc
     7201 tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt cagtttcttt
     7261 ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga actatgcgct
     7321 cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg taatcatttg
     7381 ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt
     7441 tggctttttt gttagacggc caacttttgt atacaaagtg ggccaccatg ggaatcccgc
     7501 tgctgaacgc taaacagatt ctgggacagc tggacggcgg gagcgtggca gtcctgcctg
     7561 tggtcgactc caccaatcag tacctgctgg atcgaatcgg cgagctgaag agtggggatg
     7621 cttgcattgc agaatatcag caggcaggga gaggaagcag agggaggaaa tggttctctc
     7681 cttttggagc taacctgtac ctgagtatgt tttggcgcct gaagcgggga ccagcagcaa
     7741 tcggcctggg cccggtcatc ggaattgtca tggcagaagc gctgcgaaag ctgggagcag
     7801 acaaggtgcg agtcaaatgg cccaatgacc tgtatctgca ggatagaaag ctggcaggca
     7861 tcctggtgga gctggccgga ataacaggcg atgctgcaca gatcgtcatt ggcgccggga
     7921 ttaacgtggc tatgaggcgc gtggaggaaa gcgtggtcaa tcagggctgg atcacactgc
     7981 aggaagcagg gattaacctg gacaggaata ctctggccgc tatgctgatc cgagagctgc
     8041 gggcagccct ggaactgttc gagcaggaag gcctggctcc atatctgtca cggtgggaga
     8101 agctggataa cttcatcaat agacccgtga agctgatcat tggggacaaa gagattttcg
     8161 ggattagccg ggggattgat aaacagggag ccctgctgct ggaacaggac ggagttatca
     8221 aaccctggat gggcggagaa atcagtctgc ggtctgccga aaaggctagc ggcaagccca
     8281 tccccaaccc cctgctgggc ctggacagca ccacaagttt gtacaaaaaa gcaggctcca
     8341 ccatggcggc ggccagagct actacgccgg ccgatggcga ggagcccgcc ccggaggctg
     8401 aggctctggc cgcagcccgg gagcggagca gccgcttctt gagcggcctg gagctggtga
     8461 agcagggtgc cgaggcgcgc gtgttccgtg gccgcttcca gggccgcgcg gcggtgatca
     8521 agcaccgctt ccccaagggc taccggcacc cggcgctgga ggcgcggctt ggcagacggc
     8581 ggacggtgca ggaggcccgg gcgctcctcc gctgtcgccg cgctggaata tctgccccag
     8641 ttgtcttttt tgtggactat gcttccaact gcttatatat ggaagaaatt gaaggctcag
     8701 tgactgttcg agattatatt cagtccacta tggagactga aaaaactccc cagggtctct
     8761 ccaacttagc caagacaatt gggcaggttt tggctcgaat gcacgatgaa gacctcattc
     8821 atggtgatct caccacctcc aacatgctcc tgaaaccccc cctggaacag ctgaacattg
     8881 tgctcataga ctttgggctg agtttcattt cagcacttcc agaggataag ggagtagacc
     8941 tctatgtcct ggagaaggcc ttcctcagta cccatcccaa cactgaaact gtgtttgaag
     9001 cctttctgaa gagctactcc acctcctcca aaaaggccag gccagtgcta aaaaaattag
     9061 atgaagtgcg cctgagagga agaaagaggt ccatggttgg gtaggaccca gctttcttgt
     9121 acaaagtggt gatatccagc acagtggcgg ccgctcgaca atcaacctct ggattacaaa
     9181 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     9241 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     9301 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     9361 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     9421 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     9481 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     9541 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     9601 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     9661 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     9721 cggatctccc tttgggccgc ctccccgcct ggaattctac cgggtagggg aggcgctttt
     9781 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     9841 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     9901 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     9961 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
    10021 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
    10081 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
    10141 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
    10201 cacgcttcaa aagcgcacgt ctgccg
//
