LOCUS       sequence-341992-        6604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Rosonina E, Ip JY, Calarco JA, Bakowski MA, Emili A, McCracken S, 
            Tucker P, Ingles CJ, Blencowe BJ
  TITLE     Role for PSF in mediating transcriptional activator-dependent 
            stimulation of pre-mRNA processing in vivo.
  JOURNAL   Mol Cell Biol. 2005 Aug;25(15):6734-46.
  PUBMED    16024807
REFERENCE   2  (bases 1 to 6604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-08"; volume: "25(15)"; pages: "6734-46"
COMMENT     Sequence Label: Myc-PSF-WT
FEATURES             Location/Qualifiers
     source          1..6604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             633..662
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(2820..2836)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2820..2836)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     intron          2945..3010
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3140..3160
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3585..3719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3622..3641)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3676..3695
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(3735..3753)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3774..3790)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3774..3790)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3787..3809)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3798..3814
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3822..3828,3829..3846,3847..3852))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3867..3888
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4005..4022)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4176..4764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4256..4275)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4935..5726,5727..5795))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5558..5577
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5796..5900)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5926..6381)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6063..6084)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6275..6294
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6508..6530
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6522..6539
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6523..6539
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6546..6565
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6546..6564
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta tggaacaaaa actcatctca gaagaggatc
      661 tgaagctcat gtctcgggat cggttccgga gtcgtggcgg tggcggtggt ggcttccaca
      721 ggcgtggagg aggcggcggc cgcggcggcc tccacgactt ccgttctccg ccgcccggca
      781 tgggcctcaa tcagaatcgc ggccccatgg gtcctggccc gggccagagc ggccctaagc
      841 ctccgatccc gccaccgcct ccacaccaac agcagcaaca gccaccaccg cagcagccac
      901 cgccgcagca gccgccaccg catcagccgc cgccgcatcc acagccgcat cagcagcagc
      961 agccgccgcc accgccgcag gactcttcca agcccgtcgt tgctcaggga cccggccccg
     1021 ctcccggagt aggcagcgca ccaccagcct ccagctcggc cccgcccgcc actccaccaa
     1081 cctcgggggc cccgccaggg tccgggccag gcccgactcc gaccccgccg cctgcagtca
     1141 cctcggcccc tcccggggcg ccgccaccca ccccgccaag cagcggggtc cctaccacac
     1201 ctcctcaggc cggaggcccg ccgcctccgc ccgcggcagt cccgggcccg ggtccagggc
     1261 ctaagcaggg cccaggtccg ggtggtccca aaggcggcaa aatgcctggc gggccgaagc
     1321 caggtggcgg cccgggccta agtacgcctg gcggccaccc caagccgccg catcgaggcg
     1381 gcggggagcc ccgcgggggc cgccagcacc acccgcccta ccaccagcag catcaccagg
     1441 ggcccccgcc cggcgggccc ggcggccgca gcgaggagaa gatctcggac tcggaggggt
     1501 ttaaagccaa tttgtctctc ttgaggaggc ctggagagaa aacttacaca cagcgatgtc
     1561 ggttgtttgt tgggaatcta cctgctgata tcacggagga tgaattcaaa agactatttg
     1621 ctaaatatgg agaaccagga gaagttttta tcaacaaagg caaaggattc ggatttatta
     1681 agcttgaatc tagagctttg gctgaaattg ccaaagccga actggatgat acacccatga
     1741 gaggtagaca gcttcgagtt cgctttgcca cacatgctgc tgccctttct gttcgtaatc
     1801 tttcacctta tgtttccaat gaactgttgg aagaagcctt tagccaattt ggtcctattg
     1861 aaagggctgt tgtaatagtg gatgatcgtg gaagatctac agggaaaggc attgttgaat
     1921 ttgcttctaa gccagcagca agaaaggcat ttgaacgatg cagtgaaggt gttttcttac
     1981 tgacgacaac tcctcgtcca gtcattgtgg aaccacttga acaactagat gatgaagatg
     2041 gtcttcctga aaaacttgcc cagaagaatc caatgtatca aaaggagaga gaaacccctc
     2101 ctcgttttgc ccagcatggc acgtttgagt acgaatattc tcagcgatgg aagtctttgg
     2161 atgaaatgga aaaacagcaa agggaacaag ttgaaaaaaa catgaaagat gcaaaagaca
     2221 aattggaaag tgaaatggaa gatgcctatc atgaacatca ggcaaatctt ttgcgccaag
     2281 atctgatgag acgacaggaa gaattaagac gcatggaaga acttcacaat caagaaatgc
     2341 agaaacgtaa agaaatgcaa ttgaggcaag aggaggaacg acgtagaaga gaggaagaga
     2401 tgatgattcg tcaacgtgag atggaagaac aaatgaggcg ccaaagagag gaaagttaca
     2461 gccgaatggg ctacatggat ccacgggaaa gagacatgcg aatgggtggc ggaggagcaa
     2521 tgaacatggg agatccctat ggttcaggag gccagaaatt tccacctcta ggaggtggtg
     2581 gtggcatagg ttatgaagct aatcctggcg ttccaccagc aaccatgagt ggttccatga
     2641 tgggaagtga catgcgtact gagcgctttg ggcagggagg tgcggggcct gtgggtggac
     2701 agggtcctag aggaatgggg cctggaactc cagcaggata tggtagaggg agagaagagt
     2761 acgaaggccc aaacaaaaaa ccccgatttt agctgatatc gaattcctgc agcccggggg
     2821 atccactagt tctagatgac tgactgagcg gccgccaccg cggtggcaga tctttgtgaa
     2881 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     2941 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     3001 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     3061 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     3121 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
     3181 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
     3241 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
     3301 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac
     3361 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag
     3421 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga
     3481 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     3541 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     3601 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3661 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
     3721 gccagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag
     3781 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     3841 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     3901 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     3961 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     4021 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     4081 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     4141 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4201 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4261 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4321 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4381 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4441 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4501 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4561 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     4621 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4681 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4741 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     4801 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     4861 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     4921 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     4981 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     5041 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     5101 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     5161 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     5221 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     5281 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     5341 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     5401 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     5461 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     5521 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     5581 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     5641 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     5701 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     5761 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     5821 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     5881 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
     5941 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     6001 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     6061 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     6121 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     6181 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     6241 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     6301 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     6361 atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc
     6421 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     6481 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     6541 aattgtaata cgactcacta tagggcgaat tgggtaccgg gccccccctc gagatatacg
     6601 cgtt
//
