LOCUS       sequence-342315-        3198 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EphrinA1 RTK fusion to Cry2olig - RTK domain only.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Wurz AI, Zheng KS, Hughes RM
  TITLE     Optogenetic Regulation of EphA1 RTK Activation and Signaling.
  JOURNAL   bioRxiv [Preprint]. 2024 Feb 7:2024.02.06.579139. doi: 
            10.1101/2024.02.06.579139.
  PUBMED    38370612
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Feb 7:2024.02.06.579139. doi: 
            10.1101/2024.02.06.579139."
COMMENT     Sequence Label: EphA1-RTK-Cry2olig
FEATURES             Location/Qualifiers
     source          1..3198
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             970..2463
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTRGAQIMIGAA"
     regulatory      2482..2491
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2488..3198
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2633..2651)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2901..2920
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggaccctg cacagggagc cttggacttt acccgggagc ttgatccagc gtggctgatg
       61 gtggacactg tcataggaga aggagagttt ggggaagtgt atcgagggac cctgaggctc
      121 cccagccagg actgcaagac tgtggccatt aagaccttaa aagacacatc cccaggtggc
      181 cagtggtgga acttccttcg agaggcaact atcatgggcc agtttagcca cccgcatatt
      241 ctgcatctgg aaggcgtcgt cacaaagcga aagccgatca tgatcatcac agaatttatg
      301 gagaatggag ccctggatgc cttcctgagg gagcgggagg accagctggt ccctgggcag
      361 ctagtggcca tgctgcaggg catagcatct ggcatgaact acctcagtaa tcacaattat
      421 gtccaccggg acctggctgc cagaaacatc ttggtgaatc aaaacctgtg ctgcaaggtg
      481 tctgactttg gcctgactcg cctcctggat gactttgatg gcacatacga aacccaggga
      541 ggaaagatcc ctatccgttg gacagcccct gaagccattg cccatcggat cttcaccaca
      601 gccagcgatg tgtggagctt tgggattgtg atgtgggagg tgctgagctt tggggacaag
      661 ccttatgggg agatgagcaa tcaggaggtt atgaagagca ttgaggatgg gtaccggttg
      721 ccccctcctg tggactgccc tgcccctctg tatgagctca tgaagaactg ctgggcatat
      781 gaccgtgccc gccggccaca cttccagaag cttcaggcac atctggagca actgcttgcc
      841 aacccccact ccctgcggac cattgccaac tttgacccca gggtgactct tcgcctgccc
      901 agcctgagtg gctcagatgg gggcggcagc gactcgctag cgctaccgga ctcagatctc
      961 gaggccacca tgaagatgga caaaaagact atagtttggt ttagaagaga cctaaggatt
     1021 gaggataatc ctgcattagc agcagctgct cacgaaggat ctgtttttcc tgtcttcatt
     1081 tggtgtcctg aagaagaagg acagttttat cctggaagag cttcaagatg gtggatgaaa
     1141 caatcacttg ctcacttatc tcaatccttg aaggctcttg gatctgacct cactttaatc
     1201 aaaacccaca acacgatttc agcgatcttg gattgtatcc gcgttaccgg tgctacaaaa
     1261 gtcgtcttta accacctcta tgatcctgtt tcgttagttc gggaccatac cgtaaaggag
     1321 aagctggtgg aacgtgggat ctctgtgcaa agctacaatg gagatctatt gtatgaaccg
     1381 tgggagatat actgcgaaaa gggcaaacct tttacgagtt tcaattctta ctggaagaaa
     1441 tgcttagata tgtcgattga atccgttatg cttcctcctc cttggcggtt gatgccaata
     1501 actgcagcgg ctgaagcgat ttgggcgtgt tcgattgaag aactagggct ggagaatgag
     1561 gccgagaaac cgagcaatgc gttgttaact agagcttggt ctccaggatg gagcaatgct
     1621 gataagttac taaatgagtt catcgagaag cagttgatag attatgcaaa gaacagcaag
     1681 aaagttgttg ggaattctac ttcactactt tctccgtatc tccatttcgg ggaaataagc
     1741 gtcagacacg ttttccagtg tgcccggatg aaacaaatta tatgggcaag agataagaac
     1801 agtgaaggag aagaaagtgc agatcttttt cttaggggaa tcggtttaag agagtattct
     1861 cggtatatat gtttcaactt cccgtttact cacgagcaat cgttgttgag tcatcttcgg
     1921 tttttccctt gggatgctga tgttgataag ttcaaggcct ggagacaagg caggaccggt
     1981 tatccgttgg tggatgccgg aatgagagag ctttgggcta ccggatggat gcataacaga
     2041 ataagagtga ttgtttcaag ctttgctgtg aagtttcttc tccttccatg gaaatgggga
     2101 atgaagtatt tctgggatac acttttggat gctgatttgg aatgtgacat ccttggctgg
     2161 cagtatatct ctgggagtat ccccgatggc cacgagcttg atcgcttgga caatcccgcg
     2221 ttacaaggcg ccaaatatga cccagaaggt gagtacataa ggcaatggct tcccgagctt
     2281 gcgagattgc caactgaatg gatccatcat ccatgggacg ctcctttaac cgtactcaaa
     2341 gcttctggtg tggaactcgg aacaaactat gcgaaaccca ttgtagacat cgacacagct
     2401 cgtgagctac tagctaaagc tatttcaaga acccgtggag cacagatcat gatcggagca
     2461 gcagcccggg atccaccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc
     2521 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     2581 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     2641 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     2701 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     2761 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     2821 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     2881 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     2941 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     3001 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     3061 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     3121 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     3181 gacgagctgt acaagtaa
//
